huntingtin (specific for expanded polyQ by western blot) | Developmental Studies Hybridoma Bank MW1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

huntingtin (specific for expanded polyQ by western blot)

catalog :

MW1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MW1

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:500	Macdonald D, Tessari M, Boogaard I, Smith M, Pulli K, Szynol A, et al. Quantification assays for total and polyglutamine-expanded huntingtin proteins. PLoS ONE. 2014;9:e96854 pubmed publisher
	Lin H, Boatz J, Krabbendam I, Kodali R, Hou Z, Wetzel R, et al. Fibril polymorphism affects immobilized non-amyloid flanking domains of huntingtin exon1 rather than its polyglutamine core. Nat Commun. 2017;8:15462 pubmed publisher
	Southwell A, Smith S, Davis T, Caron N, Villanueva E, Xie Y, et al. Ultrasensitive measurement of huntingtin protein in cerebrospinal fluid demonstrates increase with Huntington disease stage and decrease following brain huntingtin suppression. Sci Rep. 2015;5:12166 pubmed publisher
	Szlachcic W, Switonski P, Krzyzosiak W, Figlerowicz M, Figiel M. Huntington disease iPSCs show early molecular changes in intracellular signaling, the expression of oxidative stress proteins and the p53 pathway. Dis Model Mech. 2015;8:1047-57 pubmed publisher
	Cicchetti F, Lacroix S, Cisbani G, Vallières N, Saint Pierre M, St Amour I, et al. Mutant huntingtin is present in neuronal grafts in Huntington disease patients. Ann Neurol. 2014;76:31-42 pubmed publisher
	Landles C, Weiss A, Franklin S, Howland D, Bates G. Caspase-6 does not contribute to the proteolysis of mutant huntingtin in the HdhQ150 knock-in mouse model of Huntington's disease. PLoS Curr. 2012;4:e4fd085bfc9973 pubmed publisher
	Miller J, Arrasate M, Brooks E, Libeu C, Legleiter J, Hatters D, et al. Identifying polyglutamine protein species in situ that best predict neurodegeneration. Nat Chem Biol. 2011;7:925-34 pubmed publisher
	Landles C, Sathasivam K, Weiss A, Woodman B, Moffitt H, Finkbeiner S, et al. Proteolysis of mutant huntingtin produces an exon 1 fragment that accumulates as an aggregated protein in neuronal nuclei in Huntington disease. J Biol Chem. 2010;285:8808-23 pubmed publisher
	Raspe M, Gillis J, Krol H, Krom S, Bosch K, van Veen H, et al. Mimicking proteasomal release of polyglutamine peptides initiates aggregation and toxicity. J Cell Sci. 2009;122:3262-71 pubmed publisher
	Legleiter J, Lotz G, Miller J, Ko J, Ng C, Williams G, et al. Monoclonal antibodies recognize distinct conformational epitopes formed by polyglutamine in a mutant huntingtin fragment. J Biol Chem. 2009;284:21647-58 pubmed publisher
	Khoshnan A, Ko J, Patterson P. Effects of intracellular expression of anti-huntingtin antibodies of various specificities on mutant huntingtin aggregation and toxicity. Proc Natl Acad Sci U S A. 2002;99:1002-7 pubmed
	Ko J, Ou S, Patterson P. New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins. Brain Res Bull. 2001;56:319-29 pubmed

product information

Internal ID :

3963

Name :

MW1

Depositor Name :

Patterson, P.H.

Depositor Institution :

California Institute of Technology

Date Deposited :

1/23/03

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

huntingtin (specific for expanded polyQ by western blot)

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG2b

Isotype for catalog (legacy) :

IgG2b, kappa light chain

Positive Tested Species Reactivity :

Human

Species Tested (legacy) :

mouse and human

Initial Publication Pubmed ID :

11719267

Depositor Notes (Special Instructions) :

MW1 is specific for the elongated polyQ stretch through which mHtt aggregates. The epitope is hidden in aggregates. This antibody is also known as anti-polyQ.

Collections :

Human,Neurodevelopment

Search Keywords :

Patterson, P.H., huntingtin (specific for expanded polyQ by western blot), Human, MIgG2b, Human, HTT, HD, IT15, AB_528290, monoclonal, Human/Neurodevelopment, Immunoprecipitation/FACS/Immunofluorescence/Western Blot/ELISA/Function Blocking

Antigen Molecular Weight :

Apparent: 348 kDa

Gene :

HTT

Alternate Gene Name(s) :

HD, IT15

Uniprot ID :

P42858

Antibody Registry ID :

AB_528290

Immunogen :

Recombinant DRPLA-19Q/GST fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

HL-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

N-terminal, polyQ

Epitope Map PubMed ID :

11719267

Recommended Applications :

ELISA,FACS,Function Blocking,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

11719267 19690053 19491400 22037470

Western Blot Pubmed IDs :

11719267 24798518 19491400 20086007 22919566

Immunoprecipitation Pubmed IDs :

26174131 20086007

ELISA Pubmed IDs :

11719267

Function Blocking Pubmed IDs :

19491400

FACS Pubmed IDs :

26174131

Pubmed IDs :

11792860 28537272

Additional Information :

Appears to recognize an altered conformation of the polyQ domain generated as the polyQ domain of Htt increases in length. Time-resolved Förster resonance energy transfer (TR-FRET) assay has been developed using MW1 to assay soluble huntingtin. PMID: 22365609 23966247 23626768 23100324 22984513. RRID:AB_528290

DSHB Growth Medium :

Iscove's

References (legacy) :

Brain Res. Bull. 56(3-4), 319-329.; Proc. Natl. Acad. Sci. USA 99(2), 1002-1007.; J. Cell Sci. 122, 3262-3271.

more info or order :

Developmental Studies Hybridoma Bank product webpage