Osteopontin | Developmental Studies Hybridoma Bank MPIIIB10(1) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Osteopontin

catalog :

MPIIIB10(1)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MPIIIB10(1)

reactivity :

human, mouse, rat, dog, chicken

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS, immunohistochemistry - paraffin section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; chicken; fig 4	Stanic K, Saldivia N, Förstera B, Torrejon M, Montecinos H, Caprile T. Expression Patterns of Extracellular Matrix Proteins during Posterior Commissure Development. Front Neuroanat. 2016;10:89 pubmed
immunocytochemistry; mouse; 1:2000; fig 2 western blot; mouse; 1:50,000; fig 5f	Qian G, Fan W, Ahlemeyer B, Karnati S, Baumgart Vogt E. Peroxisomes in Different Skeletal Cell Types during Intramembranous and Endochondral Ossification and Their Regulation during Osteoblast Differentiation by Distinct Peroxisome Proliferator-Activated Receptors. PLoS ONE. 2015;10:e0143439 pubmed publisher
immunohistochemistry - paraffin section; rat; 1:300; fig 3	Frenay A, Yazdani S, Boersema M, van der Graaf A, Waanders F, van den Born J, et al. Incomplete Restoration of Angiotensin II-Induced Renal Extracellular Matrix Deposition and Inflammation Despite Complete Functional Recovery in Rats. PLoS ONE. 2015;10:e0129732 pubmed publisher
immunocytochemistry; human; 1:5	Pautke C, Schieker M, Tischer T, Kolk A, Neth P, Mutschler W, et al. Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts. Anticancer Res. 2004;24:3743-8 pubmed
	Rosa A, Kato R, Castro Raucci L, Teixeira L, De Oliveira F, Bellesini L, et al. Nanotopography drives stem cell fate toward osteoblast differentiation through ?1?1 integrin signaling pathway. J Cell Biochem. 2014;115:540-8 pubmed publisher
	Shimizu T, Nakano K, Tsujigiwa H, Nagatsuka H, Watanabe T, Okafuji N, et al. Notch signaling in mandibular condylar cartilage development. Eur J Med Res. 2007;12:515-9 pubmed
	Amir L, Li G, Schoenmaker T, Everts V, Bronckers A. Effect of thrombin peptide 508 (TP508) on bone healing during distraction osteogenesis in rabbit tibia. Cell Tissue Res. 2007;330:35-44 pubmed
	Schwertfeger K, Xian W, Kaplan A, Burnett S, Cohen D, Rosen J. A critical role for the inflammatory response in a mouse model of preneoplastic progression. Cancer Res. 2006;66:5676-85 pubmed
	Shimizu T, Tsujigiwa H, Nagatsuka H, Okafuji N, Kurihara S, Nagai N, et al. Expression of Notch1 and Math1 in mandibular condyle cartilage in neonatal mice. Angle Orthod. 2005;75:993-5 pubmed
	Kawakami T, Shimizu M, Shimizu T. Immunohistochemical characteristics of developing mandibular angle in fetal mice. Eur J Med Res. 2005;10:547-8 pubmed
	von Gertten C, Flores Morales A, Holmin S, Mathiesen T, Nordqvist A. Genomic responses in rat cerebral cortex after traumatic brain injury. BMC Neurosci. 2005;6:69 pubmed
	Zerbo I, Bronckers A, de Lange G, Burger E. Localisation of osteogenic and osteoclastic cells in porous beta-tricalcium phosphate particles used for human maxillary sinus floor elevation. Biomaterials. 2005;26:1445-51 pubmed

product information

Internal ID :

2273

Name :

MPIIIB10(1)

Depositor Name :

Solursh, M. / Franzen, A.

Depositor Institution :

University of Iowa

Date Deposited :

7/11/89

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Osteopontin

Antigen Species :

Rat

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Canine,Human,Mink,Mouse,Rabbit,Rat,Turkey

Species Tested (legacy) :

rat, human, rabbit, mouse

Initial Publication Pubmed ID :

2394708

Depositor Notes (Special Instructions) :

Osteopontin is a bone matrix protein and a cytokine secreted by activated T cells. Osteopontin is also known as bone sialoprotein 1, secreted phosphoprotein 1 and early T-lymphocyte activation 1.

Collections :

Cytoskeleton,Extracellular matrix proteins,Human,Skeletal muscle

Search Keywords :

Solursh, M./Fransen, A., Osteopontin , Rat, MIgG1, kappa light chain, Rat/Human/Rabbit/Mouse/Canine/Turkey/Mink, Spp1, OSP, Spp-1, AB_2286610, Monoclonal , Human/Extracellular matrix proteins, ELISA/Western blot/Immunohistochemistry/Immunofluorescence/Immunoprecipitation/FFPE/Function Blocking/FACS

Antigen Molecular Weight :

34.9 kDa

Gene :

Spp1

Alternate Gene Name(s) :

OSP, Spp-1

Uniprot ID :

P08721

Antibody Registry ID :

AB_2286610

Immunogen :

Bone protein fractions

Immunogen Sequence :

Full length sequence

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Recommended Applications :

ELISA,FACS,FFPE,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

8500993 8325891 7992848 7564111 7489365 9177232 10471159 10825228 11721059 12384457 12811555 15196903 15647819 15632414 16448243 16356873 16571650 17636332 21678097 21859278 26597716 11359309 15482833 18024259 18949733

Immunofluorescence Pubmed IDs :

8425912 10471159 11500811 11505371 14699636 16318630 17615406 19341622 19465514 20021685 22310837 24122940 24478351 12704211 14585688 17031821 19159323 23088527

Western Blot Pubmed IDs :

8293561 15647819 16740705 16571650 17317783 20418863 22892131 24478351

Immunoprecipitation Pubmed IDs :

1472061 1510790 7671165

FFPE Pubmed IDs :

8500993 7992848 7564111 10471159 12811555 15647819 16448243 16571650 17636332 15482833

Function Blocking Pubmed IDs :

23610145 26597716 17574222 22214933

FACS Pubmed IDs :

25406169

Pubmed IDs :

24122940

Additional Information :

RRID: AB_2286610

DSHB Growth Medium :

RPMI

References (legacy) :

J. Biol. Chem. 265, 14956-14963.; J. Cell Phys. 152, 467-477.; Biochem. & Biophys. Res. Comm. 189, 892-898.; Histochemistry 99, 295-301.; J. Biol. Chem. 268, 15180-15184.; J. Cell. Phys. 154, 317-328.; Am. J. Path. 145, 1450-1462.; Circ. Res. 74(2), 214-224.; Kidney Int. 48, 439-448.; Nature Medicine, 1(10), 1067-1073.; Calcif. Tissue Int. 57, 52-59.; Proc. Natl. Acad. Sci. 94, 6414-6419.; Ann. N.Y. Acad. Sci. 760, 257-265.; Arch. Oral Biol. 44, 751-757.; Clin. Imp. Dent. Rel. Res. 1(1), 33-40.; J. Biomed. Mater. Res. 51, 280-291.; Anat. Rec. 263, 350-360.; Anat. Rec. 264, 51-62.; Science 294, 1731-1735.; Frink, R.J. Inflammatory atherosclerosis: Characteristics of the injurious agent. First Edition. The Heart Research Foundation of Sacramento, 2002.; Virchows Arch. 443, 206-216.; J. Histochem. Cytochem. 51(5), 633-641.; Biomaterials 25, 403-413.; Curr. Top. Microbiol. Immunol. 280, 71-109.; Anat. Rec. Part A 276A, 75-102.; Eur. J. Med. Res. 9, 533-535.; Res. Vet. Sci. 77, 131-141.; Biomaterials 26, 1445-1451.; Eur. J. Med. Res. 10, 475-479.; Eur. J. Med. Res. 10, 547-548.; Angle Orthod. 75(6), 993-995.; Cancer Res. 66(11), 5676-5685.; J Biomed. Mater. Res. A 80A(3), 554-564.; Eur. J. Med. Res. 12, 515-519.; Cardiovasc. Res. 75, 738-747.; Eur. J. Med. Res. 13, 4-6.; J. Nephrol(more. )

more info or order :

Developmental Studies Hybridoma Bank product webpage