product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
endoglin (CD105)
catalog :
MJ7/18
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
MJ7/18
reactivity :
mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS
citations: 23
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; fig 5c
Muenzner P, Kengmo Tchoupa A, Klauser B, Brunner T, Putze J, Dobrindt U, et al. Uropathogenic E. coli Exploit CEA to Promote Colonization of the Urogenital Tract Mucosa. PLoS Pathog. 2016;12:e1005608 pubmed publisher
Choquet C, Nguyen T, Sicard P, Buttigieg E, Tran T, Kober F, et al. Deletion of Nkx2-5 in trabecular myocardium reveals the developmental origins of pathological heterogeneity associated with ventricular non-compaction cardiomyopathy. PLoS Genet. 2018;14:e1007502 pubmed publisher
Karmani L, Leveque P, Bouzin C, Bol A, Dieu M, Walrand S, et al. Biodistribution of (125)I-labeled anti-endoglin antibody using SPECT/CT imaging: Impact of in vivo deiodination on tumor accumulation in mice. Nucl Med Biol. 2016;43:415-23 pubmed publisher
Mylroie H, Dumont O, Bauer A, Thornton C, Mackey J, Calay D, et al. PKCε-CREB-Nrf2 signalling induces HO-1 in the vascular endothelium and enhances resistance to inflammation and apoptosis. Cardiovasc Res. 2015;106:509-19 pubmed publisher
Karmani L, Bouchat V, Bouzin C, Leveque P, Labar D, Bol A, et al. (89)Zr-labeled anti-endoglin antibody-targeted gold nanoparticles for imaging cancer: implications for future cancer therapy. Nanomedicine (Lond). 2014;9:1923-37 pubmed publisher
Rivera L, Brekken R. SPARC promotes pericyte recruitment via inhibition of endoglin-dependent TGF-?1 activity. J Cell Biol. 2011;193:1305-19 pubmed publisher
Romero D, O Neill C, Terzic A, Contois L, Young K, Conley B, et al. Endoglin regulates cancer-stromal cell interactions in prostate tumors. Cancer Res. 2011;71:3482-93 pubmed publisher
Rowland T, Miller L, Blaschke A, Doss E, Bonham A, Hikita S, et al. Roles of integrins in human induced pluripotent stem cell growth on Matrigel and vitronectin. Stem Cells Dev. 2010;19:1231-40 pubmed publisher
Arguello A, Fischer S, Schonborn J, Markus R, Brekken R, Eisch A. Effect of chronic morphine on the dentate gyrus neurogenic microenvironment. Neuroscience. 2009;159:1003-10 pubmed publisher
Kinderlerer A, Pombo Gregoire I, Hamdulay S, Ali F, Steinberg R, Silva G, et al. Heme oxygenase-1 expression enhances vascular endothelial resistance to complement-mediated injury through induction of decay-accelerating factor: a role for increased bilirubin and ferritin. Blood. 2009;113:1598-607 pubmed publisher
Mason J, Lidington E, Yarwood H. Isolation and analysis of large and small vessel endothelial cells. Methods Mol Med. 2007;135:305-21 pubmed
Korpanty G, Carbon J, Grayburn P, Fleming J, Brekken R. Monitoring response to anticancer therapy by targeting microbubbles to tumor vasculature. Clin Cancer Res. 2007;13:323-30 pubmed
Korpanty G, Grayburn P, Shohet R, Brekken R. Targeting vascular endothelium with avidin microbubbles. Ultrasound Med Biol. 2005;31:1279-83 pubmed
Redaelli C, Semela D, Carrick F, Ledermann M, Candinas D, Sauter B, et al. Effect of vascular endothelial growth factor on functional recovery after hepatectomy in lean and obese mice. J Hepatol. 2004;40:305-12 pubmed
Ahmad S, Lidington E, Ohta R, Okada N, Robson M, Davies K, et al. Decay-accelerating factor induction by tumour necrosis factor-alpha, through a phosphatidylinositol-3 kinase and protein kinase C-dependent pathway, protects murine vascular endothelial cells against complement deposition. Immunology. 2003;110:258-68 pubmed
Garton K, Gough P, Philalay J, Wille P, Blobel C, Whitehead R, et al. Stimulated shedding of vascular cell adhesion molecule 1 (VCAM-1) is mediated by tumor necrosis factor-alpha-converting enzyme (ADAM 17). J Biol Chem. 2003;278:37459-64 pubmed
Charbord P, Oostendorp R, Pang W, Herault O, Noel F, Tsuji T, et al. Comparative study of stromal cell lines derived from embryonic, fetal, and postnatal mouse blood-forming tissues. Exp Hematol. 2002;30:1202-10 pubmed
Lidington E, Rao R, Marelli Berg F, Jat P, Haskard D, Mason J. Conditional immortalization of growth factor-responsive cardiac endothelial cells from H-2K(b)-tsA58 mice. Am J Physiol Cell Physiol. 2002;282:C67-74 pubmed
Izawa D, Tanaka T, Saito K, Ogihara H, Usui T, Kawamoto S, et al. Expression profile of active genes in mouse lymph node high endothelial cells. Int Immunol. 1999;11:1989-98 pubmed
Kruse A, Hallmann R, Butcher E. Specialized patterns of vascular differentiation antigens in the pregnant mouse uterus and the placenta. Biol Reprod. 1999;61:1393-401 pubmed
Ge A, Butcher E. Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand. Gene. 1994;138:201-6 pubmed
Hallmann R, Mayer D, Berg E, Broermann R, Butcher E. Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier. Dev Dyn. 1995;202:325-32 pubmed
Engelhardt B, Conley F, Butcher E. Cell adhesion molecules on vessels during inflammation in the mouse central nervous system. J Neuroimmunol. 1994;51:199-208 pubmed
product information
Internal ID :
1642
Name :
MJ7/18
Depositor Name :
Butcher, E.C. / Jutila, M.
Depositor Institution :
Stanford University School of Medicine and Montana State University
Date Deposited :
8/4/95
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
endoglin (CD105)
Antigen Species :
Rat
Host Species :
rat
Isotype :
RIgG2a
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Mouse
Species Tested (legacy) :
mouse; other species not tested
Initial Publication Pubmed ID :
8125301
Depositor Notes (Special Instructions) :
Pan endothelial marker with an extremely weak (minimal) reactivity with early hematopoetic cells (J. Spangrude, personal communication).
Collections :
CD antigens
Search Keywords :
Butcher, E.C. / Jutila, M., endoglin (CD105), Rat, RIgG2a, Mouse, Eng, CD105, Edg, Endo, AB_2262267, monoclonal, Cluster Determinant antigens, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/FACS
Antigen Molecular Weight :
Predicted: 70 kDa; Apparent: 180 kDa (nonreduced) or 90 (reduced)
Gene :
Eng
Alternate Gene Name(s) :
CD105, Edg, Endo
Uniprot ID :
Q63961
Antibody Registry ID :
AB_2262267
Synonyms (Alternate Clone Names) :
CD105
Immunogen :
Inflamed mouse skin
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
SP2/0
Epitope Mapped :
Yes
Epitope Location or Sequence :
extracellular domain
Epitope Map PubMed ID :
8125301
Recommended Applications :
FACS,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunohistochemistry Pubmed IDs :
7626790 7514186 21444673 14739103
Immunofluorescence Pubmed IDs :
10569981 16176794 17200371 21708981 21444673 19356684 12384152
Western Blot Pubmed IDs :
8125301 21708981 12384152
Immunoprecipitation Pubmed IDs :
21708981
FACS Pubmed IDs :
8125301 16176794 11742799 10590265 19036700 12384152
Pubmed IDs :
27179250 25883219 19811096 24547782 12878595 14511240 17951667
Additional Information :
Endoglin is a major glycoprotein of vascular endothelium. MJ7/18 is commonly used for the isolation of murine endothelial cells.
DSHB Growth Medium :
RPMI
References (legacy) :
Gene 138, 201-206.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US