Integrin alpha-L; CD11a | Developmental Studies Hybridoma Bank MHM.24 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Integrin alpha-L; CD11a

catalog :

MHM.24

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MHM.24

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation, FACS, blocking or activating experiments

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 4a	Eppler F, Quast T, Kolanus W. Dynamin2 controls Rap1 activation and integrin clustering in human T lymphocyte adhesion. PLoS ONE. 2017;12:e0172443 pubmed publisher
blocking or activating experiments; human; 10 ug/ml; loading ...; fig 3b	Kawamoto E, Okamoto T, Takagi Y, Honda G, Suzuki K, Imai H, et al. LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin. Biochem Biophys Res Commun. 2016;473:1005-1012 pubmed publisher
	Thinn A, Wang Z, Zhou D, Zhao Y, Curtis B, Zhu J. Autonomous conformational regulation of β3 integrin and the conformation-dependent property of HPA-1a alloantibodies. Proc Natl Acad Sci U S A. 2018;115:E9105-E9114 pubmed publisher
	Jahan F, Madhavan S, Rolova T, Viazmina L, Grönholm M, Gahmberg C. Phosphorylation of the α-chain in the integrin LFA-1 enables β2-chain phosphorylation and α-actinin binding required for cell adhesion. J Biol Chem. 2018;293:12318-12330 pubmed publisher
	Huang L, Shimaoka M, Rondon I, Roy I, Chang Q, Po M, et al. Identification and characterization of a human monoclonal antagonistic antibody AL-57 that preferentially binds the high-affinity form of lymphocyte function-associated antigen-1. J Leukoc Biol. 2006;80:905-14 pubmed

product information

Internal ID :

4131

Name :

MHM.24

Depositor Name :

Hildreth, J.E.K.

Depositor Institution :

Johns Hopkins University School of Medicine

Date Deposited :

4/12/93

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

Integrin alpha-L; CD11a

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human

Species Tested (legacy) :

human--no cross reactivities noted to date

Initial Publication Pubmed ID :

6339253

Depositor Notes (Special Instructions) :

CD11a, as as component of the LFA-1 integrin, is the receptor for ISG15 protein (PMID: 29100055) to signal cytokine secretion. This antibody does recognizes CD11a in acetone-fixed frozen sections but fails in all aldehyde-fixation protocols.

Collections :

CD antigens,Cell adhesion,Cell markers,Cell signaling,Human

Search Keywords :

Hildreth, J.E.K., Integrin alpha-L; CD11a, Human, MIgG1, kappa light chain, Human, CD11A, LFA-1, AB_2265116, Monoclonal , Human Cell markers CD antigens Cell adhesion Cell signaling, Immunoprecipitation Western blot Function Blocking Immunohistochemistry FACS

Antigen Molecular Weight :

175 kD

Gene :

CD11A

Alternate Gene Name(s) :

LFA-1

Uniprot ID :

P20701

Antibody Registry ID :

AB_2265116

Immunogen :

EBV-transformed human lymphoblastoid cells

Immunogen Sequence :

Full length sequence

Clonality :

Monoclonal

Myeloma Strain :

P3-NSI/1Ag4.1

Epitope Mapped :

Yes

Epitope Location or Sequence :

alpha chain I domain; a.a.197-201

Epitope Map PubMed ID :

27443292

Recommended Applications :

FACS,Function Blocking,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

6339253

Immunofluorescence Pubmed IDs :

16888085 31315993

Western Blot Pubmed IDs :

29100055 6339253

Immunoprecipitation Pubmed IDs :

2481234 2580022 6339253

Function Blocking Pubmed IDs :

16888085 3930261 27443292

FACS Pubmed IDs :

16888085 6339253

Additional Information :

RRID: AB_2265116

DSHB Growth Medium :

Iscove's

References (legacy) :

Eur. J. Immunol. 13, 202-208.; J. Immunol. 134, 3272-3280.; Eur. J. Immunol. 15, 888-892.; EMBO J. 6, 915-919.; Mol. Immunol. 26, 883-895.; J. Leukoc. Biol. 80, 905-914.

more info or order :

Developmental Studies Hybridoma Bank product webpage