product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Apical junction molecule-1 (cell-junction marker ajm-1)
catalog :
MH27
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MH27
reactivity :
Caenorhabditis elegans
application :
western blot, immunohistochemistry, immunocytochemistry
citations: 22
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; Caenorhabditis elegans; 1:20; loading ...; fig s2
van Rijnberk L, van der Horst S, van den Heuvel S, Ruijtenberg S. A dual transcriptional reporter and CDK-activity sensor marks cell cycle entry and progression in C. elegans. PLoS ONE. 2017;12:e0171600 pubmed publisher
  • immunohistochemistry; Caenorhabditis elegans; 1:500; fig 2
Smurova K, Podbilewicz B. RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion. Cell Rep. 2016;14:1517-1527 pubmed publisher
  • immunocytochemistry; Caenorhabditis elegans; 1:100; fig 3B
Gorrepati L, Krause M, Chen W, Brodigan T, Correa Mendez M, Eisenmann D. Identification of Wnt Pathway Target Genes Regulating the Division and Differentiation of Larval Seam Cells and Vulval Precursor Cells in Caenorhabditis elegans. G3 (Bethesda). 2015;5:1551-66 pubmed publisher
  • immunocytochemistry; Caenorhabditis elegans; 1:20; loading ...; fig s4a
The I, Ruijtenberg S, Bouchet B, Cristobal A, Prinsen M, van Mourik T, et al. Rb and FZR1/Cdh1 determine CDK4/6-cyclin D requirement in C. elegans and human cancer cells. Nat Commun. 2015;6:5906 pubmed publisher
Walck Shannon E, Lucas B, Chin Sang I, Reiner D, Kumfer K, Cochran H, et al. CDC-42 Orients Cell Migration during Epithelial Intercalation in the Caenorhabditis elegans Epidermis. PLoS Genet. 2016;12:e1006415 pubmed publisher
Izumikawa T, Dejima K, Watamoto Y, Nomura K, Kanaki N, Rikitake M, et al. Chondroitin 4-O-Sulfotransferase Is Indispensable for Sulfation of Chondroitin and Plays an Important Role in Maintaining Normal Life Span and Oxidative Stress Responses in Nematodes. J Biol Chem. 2016;291:23294-23304 pubmed
Gómez Saldivar G, Fernandez A, Hirano Y, Mauro M, Lai A, Ayuso C, et al. Identification of Conserved MEL-28/ELYS Domains with Essential Roles in Nuclear Assembly and Chromosome Segregation. PLoS Genet. 2016;12:e1006131 pubmed publisher
Asan A, Raiders S, Priess J. Morphogenesis of the C. elegans Intestine Involves Axon Guidance Genes. PLoS Genet. 2016;12:e1005950 pubmed publisher
Waaijers S, Ramalho J, Koorman T, Kruse E, Boxem M. The C. elegans Crumbs family contains a CRB3 homolog and is not essential for viability. Biol Open. 2015;4:276-84 pubmed publisher
Martin E, Harel S, Nkengfac B, Hamiche K, Neault M, Jenna S. pix-1 controls early elongation in parallel with mel-11 and let-502 in Caenorhabditis elegans. PLoS ONE. 2014;9:e94684 pubmed publisher
Rasmussen J, Feldman J, Reddy S, Priess J. Cell interactions and patterned intercalations shape and link epithelial tubes in C. elegans. PLoS Genet. 2013;9:e1003772 pubmed publisher
Gissendanner C, Kelley T. The C. elegans gene pan-1 encodes novel transmembrane and cytoplasmic leucine-rich repeat proteins and promotes molting and the larva to adult transition. BMC Dev Biol. 2013;13:21 pubmed publisher
Bernadskaya Y, Patel F, Hsu H, Soto M. Arp2/3 promotes junction formation and maintenance in the Caenorhabditis elegans intestine by regulating membrane association of apical proteins. Mol Biol Cell. 2011;22:2886-99 pubmed publisher
Gissendanner C, Kelley K, Nguyen T, Hoener M, Sluder A, Maina C. The Caenorhabditis elegans NR4A nuclear receptor is required for spermatheca morphogenesis. Dev Biol. 2008;313:767-86 pubmed
Kim Y, Kipreos E. The Caenorhabditis elegans replication licensing factor CDT-1 is targeted for degradation by the CUL-4/DDB-1 complex. Mol Cell Biol. 2007;27:1394-406 pubmed
Koppen M, Simske J, Sims P, Firestein B, Hall D, Radice A, et al. Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia. Nat Cell Biol. 2001;3:983-91 pubmed
Legouis R, Gansmuller A, Sookhareea S, Bosher J, Baillie D, Labouesse M. LET-413 is a basolateral protein required for the assembly of adherens junctions in Caenorhabditis elegans. Nat Cell Biol. 2000;2:415-22 pubmed
Hresko M, Schriefer L, Shrimankar P, Waterston R. Myotactin, a novel hypodermal protein involved in muscle-cell adhesion in Caenorhabditis elegans. J Cell Biol. 1999;146:659-72 pubmed
Yochem J, Tuck S, Greenwald I, Han M. A gp330/megalin-related protein is required in the major epidermis of Caenorhabditis elegans for completion of molting. Development. 1999;126:597-606 pubmed
Hresko M, Williams B, Waterston R. Assembly of body wall muscle and muscle cell attachment structures in Caenorhabditis elegans. J Cell Biol. 1994;124:491-506 pubmed
Williams B, Waterston R. Genes critical for muscle development and function in Caenorhabditis elegans identified through lethal mutations. J Cell Biol. 1994;124:475-90 pubmed
Francis R, Waterston R. Muscle cell attachment in Caenorhabditis elegans. J Cell Biol. 1991;114:465-79 pubmed
product information
Internal ID :
998
Name :
MH27
Depositor Name :
Waterston, R.H.
Depositor Institution :
University of Washington
Date Deposited :
9/12/00
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Apical junction molecule-1 (cell-junction marker ajm-1)
Antigen Species :
C. elegans
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
C. elegans
Species Tested (legacy) :
Caenorhabditis elegans
Initial Publication Pubmed ID :
1860880
Depositor Notes (Special Instructions) :
MH27 stains cell-cell junctions in hypodermis, pharynx, and gut.
Collections :
C. elegans,Cell adhesion,Cytoskeleton
Search Keywords :
Waterston, R.H., Apical junction molecule-1 (cell-junction marker ajm-1), C. elegans , MIgG1, kappa light chain, C. elegans , ajm-1, , AB_531819, monoclonal, C. elegans/Cell adhesion/Cytoskeletal elements, Immunofluorescence/Western Blot
Antigen Molecular Weight :
Multiple isoforms; Predicted: from 142 to 222 kDa
Gene :
ajm-1
Uniprot ID :
A0A1C3NSP2
Antibody Registry ID :
AB_531819
Immunogen :
Crude protein fraction
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
Sp2/O-ag
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Western Blot
Immunoblotting (legacy) :
yes
Immunofluorescence Pubmed IDs :
11715019 27645998 27341616 27861585 27035721 10878806 18096150 9876188 8106547 8106548 10444073 17145765
Western Blot Pubmed IDs :
1860880
Additional Information :
There is no immunoreactivity in mutant embryos. There are multiple Uniprot entries for this protein; A0A1C3NSP2 is the ID for the largast isoform that cantains all exon products.
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Cell Biol. 114, 465-479.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US