product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Apical junction molecule-1 (cell-junction marker ajm-1)
catalog :
MH27
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MH27
reactivity :
Caenorhabditis elegans
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
citations: 22
Published Application/Species/Sample/Dilution | Reference |
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Izumikawa T, Dejima K, Watamoto Y, Nomura K, Kanaki N, Rikitake M, et al. Chondroitin 4-O-Sulfotransferase Is Indispensable for Sulfation of Chondroitin and Plays an Important Role in Maintaining Normal Life Span and Oxidative Stress Responses in Nematodes. J Biol Chem. 2016;291:23294-23304 pubmed
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Gissendanner C, Kelley K, Nguyen T, Hoener M, Sluder A, Maina C. The Caenorhabditis elegans NR4A nuclear receptor is required for spermatheca morphogenesis. Dev Biol. 2008;313:767-86 pubmed
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Kim Y, Kipreos E. The Caenorhabditis elegans replication licensing factor CDT-1 is targeted for degradation by the CUL-4/DDB-1 complex. Mol Cell Biol. 2007;27:1394-406 pubmed
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Koppen M, Simske J, Sims P, Firestein B, Hall D, Radice A, et al. Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia. Nat Cell Biol. 2001;3:983-91 pubmed
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Legouis R, Gansmuller A, Sookhareea S, Bosher J, Baillie D, Labouesse M. LET-413 is a basolateral protein required for the assembly of adherens junctions in Caenorhabditis elegans. Nat Cell Biol. 2000;2:415-22 pubmed
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Hresko M, Schriefer L, Shrimankar P, Waterston R. Myotactin, a novel hypodermal protein involved in muscle-cell adhesion in Caenorhabditis elegans. J Cell Biol. 1999;146:659-72 pubmed
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Yochem J, Tuck S, Greenwald I, Han M. A gp330/megalin-related protein is required in the major epidermis of Caenorhabditis elegans for completion of molting. Development. 1999;126:597-606 pubmed
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Hresko M, Williams B, Waterston R. Assembly of body wall muscle and muscle cell attachment structures in Caenorhabditis elegans. J Cell Biol. 1994;124:491-506 pubmed
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Williams B, Waterston R. Genes critical for muscle development and function in Caenorhabditis elegans identified through lethal mutations. J Cell Biol. 1994;124:475-90 pubmed
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Francis R, Waterston R. Muscle cell attachment in Caenorhabditis elegans. J Cell Biol. 1991;114:465-79 pubmed
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product information
Internal ID :
998
Name :
MH27
Depositor Name :
Waterston, R.H.
Depositor Institution :
University of Washington
Date Deposited :
9/12/00
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Apical junction molecule-1 (cell-junction marker ajm-1)
Antigen Species :
C. elegans
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
C. elegans
Species Tested (legacy) :
Caenorhabditis elegans
Initial Publication Pubmed ID :
1860880
Depositor Notes (Special Instructions) :
MH27 stains cell-cell junctions in hypodermis, pharynx, and gut.
Collections :
C. elegans,Cell adhesion,Cytoskeleton
Search Keywords :
Waterston, R.H., Apical junction molecule-1 (cell-junction marker ajm-1), C. elegans , MIgG1, kappa light chain, C. elegans , ajm-1, , AB_531819, monoclonal, C. elegans/Cell adhesion/Cytoskeletal elements, Immunofluorescence/Western Blot
Antigen Molecular Weight :
Multiple isoforms; Predicted: from 142 to 222 kDa
Gene :
ajm-1
Uniprot ID :
A0A1C3NSP2
Antibody Registry ID :
AB_531819
Immunogen :
Crude protein fraction
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
Sp2/O-ag
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Western Blot
Immunoblotting (legacy) :
yes
Immunofluorescence Pubmed IDs :
11715019 27645998 27341616 27861585 27035721 10878806 18096150 9876188 8106547 8106548 10444073 17145765
Western Blot Pubmed IDs :
1860880
Additional Information :
There is no immunoreactivity in mutant embryos. There are multiple Uniprot entries for this protein; A0A1C3NSP2 is the ID for the largast isoform that cantains all exon products.
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Cell Biol. 114, 465-479.
more info or order :
company information
Developmental Studies Hybridoma Bank
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