product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
plasmalemma vesicle-associated protein (PLVAP)
catalog :
MECA-32
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
MECA-32
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section, immunohistochemistry - free floating section
more info or order :
Developmental Studies Hybridoma Bank product webpage
citations: 26
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:200; fig 3
Kim D, Glendining K, Grattan D, Jasoni C. Maternal Obesity in the Mouse Compromises the Blood-Brain Barrier in the Arcuate Nucleus of Offspring. Endocrinology. 2016;157:2229-42 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:100
Morita S, Furube E, Mannari T, Okuda H, Tatsumi K, Wanaka A, et al. Heterogeneous vascular permeability and alternative diffusion barrier in sensory circumventricular organs of adult mouse brain. Cell Tissue Res. 2016;363:497-511 pubmed publisher
Guo H, Kazadaeva Y, Ortega F, Manjunath N, Desai T. Trinucleotide repeat containing 6c (TNRC6c) is essential for microvascular maturation during distal airspace sacculation in the developing lung. Dev Biol. 2017;430:214-223 pubmed publisher
Jacobi J, Garcia Barros M, Rao S, Rotolo J, Thompson C, Mizrachi A, et al. Targeting acid sphingomyelinase with anti-angiogenic chemotherapy. Cell Signal. 2017;29:52-61 pubmed publisher
Scully K, Skowronska Krawczyk D, Krawczyk M, Merkurjev D, Taylor H, Livolsi A, et al. Epithelial cell integrin β1 is required for developmental angiogenesis in the pituitary gland. Proc Natl Acad Sci U S A. 2016;113:13408-13413 pubmed
Morita Takemura S, Nakahara K, Tatsumi K, Okuda H, Tanaka T, Isonishi A, et al. Changes in endothelial cell proliferation and vascular permeability after systemic lipopolysaccharide administration in the subfornical organ. J Neuroimmunol. 2016;298:132-7 pubmed publisher
Franckaert D, Schlenner S, Heirman N, Gill J, Skogberg G, Ekwall O, et al. Premature thymic involution is independent of structural plasticity of the thymic stroma. Eur J Immunol. 2015;45:1535-47 pubmed publisher
Morita S, Furube E, Mannari T, Okuda H, Tatsumi K, Wanaka A, et al. Vascular endothelial growth factor-dependent angiogenesis and dynamic vascular plasticity in the sensory circumventricular organs of adult mouse brain. Cell Tissue Res. 2015;359:865-84 pubmed publisher
Guay J, Wojchowski D, Fang J, Oxburgh L. Death associated protein kinase 2 is expressed in cortical interstitial cells of the mouse kidney. BMC Res Notes. 2014;7:345 pubmed publisher
Blum K, Karaman S, Proulx S, Ochsenbein A, Luciani P, Leroux J, et al. Chronic high-fat diet impairs collecting lymphatic vessel function in mice. PLoS ONE. 2014;9:e94713 pubmed publisher
Umemoto E, Takeda A, Jin S, Luo Z, Nakahogi N, Hayasaka H, et al. Dynamic changes in endothelial cell adhesion molecule nepmucin/CD300LG expression under physiological and pathological conditions. PLoS ONE. 2013;8:e83681 pubmed publisher
Stancevic B, Varda Bloom N, Cheng J, Fuller J, Rotolo J, García Barros M, et al. Adenoviral transduction of human acid sphingomyelinase into neo-angiogenic endothelium radiosensitizes tumor cure. PLoS ONE. 2013;8:e69025 pubmed publisher
Couto J, Almeida A, Daly L, Sobrinho Simoes M, Bromberg J, Soares P. AZD1480 blocks growth and tumorigenesis of RET- activated thyroid cancer cell lines. PLoS ONE. 2012;7:e46869 pubmed publisher
Hamburg E, ATIT R. Sustained ?-catenin activity in dermal fibroblasts is sufficient for skin fibrosis. J Invest Dermatol. 2012;132:2469-2472 pubmed publisher
Tkachenko E, Tse D, Sideleva O, Deharvengt S, Luciano M, Xu Y, et al. Caveolae, fenestrae and transendothelial channels retain PV1 on the surface of endothelial cells. PLoS ONE. 2012;7:e32655 pubmed publisher
Chernoguz A, Crawford K, Vandersall A, Rao M, Willson T, Denson L, et al. Pretreatment with anti-VEGF therapy may exacerbate inflammation in experimental acute colitis. J Pediatr Surg. 2012;47:347-54 pubmed publisher
Furuya M, Kirschbaum S, Paulovich A, Pauli B, Zhang H, Alexander J, et al. Lymphatic endothelial murine chloride channel calcium-activated 1 is a ligand for leukocyte LFA-1 and Mac-1. J Immunol. 2010;185:5769-77 pubmed publisher
Garcia Barros M, Thin T, Maj J, Cordon Cardo C, Haimovitz Friedman A, Fuks Z, et al. Impact of stromal sensitivity on radiation response of tumors implanted in SCID hosts revisited. Cancer Res. 2010;70:8179-86 pubmed publisher
Truman J, Garcia Barros M, Kaag M, Hambardzumyan D, Stancevic B, Chan M, et al. Endothelial membrane remodeling is obligate for anti-angiogenic radiosensitization during tumor radiosurgery. PLoS ONE. 2010;5:e12310 pubmed publisher
Roland C, Dineen S, Toombs J, Carbon J, Smith C, Brekken R, et al. Tumor-derived intercellular adhesion molecule-1 mediates tumor-associated leukocyte infiltration in orthotopic pancreatic xenografts. Exp Biol Med (Maywood). 2010;235:263-70 pubmed publisher
Roland C, Lynn K, Toombs J, Dineen S, Udugamasooriya D, Brekken R. Cytokine levels correlate with immune cell infiltration after anti-VEGF therapy in preclinical mouse models of breast cancer. PLoS ONE. 2009;4:e7669 pubmed publisher
Kasten P, Schnöink G, Bergmann A, Papoutsi M, Buttler K, Rossler J, et al. Similarities and differences of human and experimental mouse lymphangiomas. Dev Dyn. 2007;236:2952-61 pubmed
Beck A, Luster T, Miller A, Holloway S, Conner C, Barnett C, et al. Combination of a monoclonal anti-phosphatidylserine antibody with gemcitabine strongly inhibits the growth and metastasis of orthotopic pancreatic tumors in mice. Int J Cancer. 2006;118:2639-43 pubmed
Hallmann R, Mayer D, Berg E, Broermann R, Butcher E. Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier. Dev Dyn. 1995;202:325-32 pubmed
Engelhardt B, Conley F, Butcher E. Cell adhesion molecules on vessels during inflammation in the mouse central nervous system. J Neuroimmunol. 1994;51:199-208 pubmed
Leppink D, Bishop D, Sedmak D, Henry M, Ferguson R, Streeter P, et al. Inducible expression of an endothelial cell antigen on murine myocardial vasculature in association with interstitial cellular infiltration. Transplantation. 1989;48:874-7 pubmed
product information
Internal ID :
1630
Name :
MECA-32
Depositor Name :
Butcher, E.C.
Depositor Institution :
Stanford University School of Medicine
Date Deposited :
8/4/95
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
plasmalemma vesicle-associated protein (PLVAP)
Antigen Species :
Mouse
Host Species :
rat
Isotype :
RIgG2a
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Human,Mouse
Species Tested (legacy) :
mouse
Initial Publication Pubmed ID :
2683264
Depositor Notes (Special Instructions) :
Binds to murine endothelial cells. most or all EC except for capillaries in skeletal and cardiac muscle, and vessels in the CNS. The antigen is downregulated during CNS development in association with formation of the blood brain barrier, and is induced in negative vessels in muscle and CNS in association with inflammation.
Collections :
Cardiac development,Cell markers,Cytoskeleton,Skeletal muscle
Search Keywords :
Butcher, E.C., plasmalemma vesicle-associated protein (PLVAP), Mouse, RIgG2a, mouse, Plvap, Pv1, AB_531797, monoclonal, Cardiac development/Cell markers/Cytoskeletal elements/Skeletal muscle, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/FFPE
Antigen Molecular Weight :
Predicted: 50 kDa; Apparent: 50-55 kDa (reducing conditions), 100-120 kDa (nonreducing)
Gene :
Plvap
Alternate Gene Name(s) :
Pv1
Uniprot ID :
Q91VC4
Antibody Registry ID :
AB_531797
Immunogen :
Lymph node stroma.
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
SP2/0
Epitope Mapped :
No
Recommended Applications :
FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunohistochemistry Pubmed IDs :
2683264 7626790 7514186 25627671 20924105 16353142 27702691 30996136
Immunofluorescence Pubmed IDs :
27609286 26048259 17879316 25627671 25573819 22325388 20404043
Western Blot Pubmed IDs :
7626790
Immunoprecipitation Pubmed IDs :
7626790
FFPE Pubmed IDs :
20924105 27702691
Additional Information :
Marker of fenestrated vasculature
DSHB Growth Medium :
RPMI
References (legacy) :
Transplantation 48, 874-877.; Dev. Dyn. 202, 325-332.
more info or order :
Developmental Studies Hybridoma Bank product webpage
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US