SSEA-4/ stage-specific embryonic antigen 4/globolipid | Developmental Studies Hybridoma Bank MC-813-70 (SSEA-4) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

SSEA-4/ stage-specific embryonic antigen 4/globolipid

catalog :

MC-813-70 (SSEA-4)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MC-813-70 (SSEA-4)

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 18

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:3; fig 1g	Alonso Barroso E, Brasil S, Briso Montiano Á, Navarrete R, Perez Cerda C, Ugarte M, et al. Generation and characterization of a human iPSC line from a patient with propionic acidemia due to defects in the PCCA gene. Stem Cell Res. 2017;23:173-177 pubmed publisher
flow cytometry; scFv; 1:100; fig s1c	Kempf H, Olmer R, Haase A, Franke A, Bolesani E, Schwanke K, et al. Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells. Nat Commun. 2016;7:13602 pubmed publisher
immunocytochemistry; scFv; 1:50; fig 4	Yuan Y, Yang Y, Tian Y, Park J, Dai A, Roberts R, et al. Efficient long-term cryopreservation of pluripotent stem cells at -80?°C. Sci Rep. 2016;6:34476 pubmed publisher
immunocytochemistry; scFv; 1:25; fig s1	Josowitz R, Mulero Navarro S, Rodriguez N, Falce C, Cohen N, Ullian E, et al. Autonomous and Non-autonomous Defects Underlie Hypertrophic Cardiomyopathy in BRAF-Mutant hiPSC-Derived Cardiomyocytes. Stem Cell Reports. 2016;7:355-369 pubmed publisher
immunohistochemistry; scFv; 1:100; loading ...; fig s9c	Hyslop L, Blakeley P, Craven L, Richardson J, Fogarty N, Fragouli E, et al. Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease. Nature. 2016;534:383-6 pubmed publisher
immunocytochemistry; scFv; 1:20; fig 5s1a	Carroll B, Maetzel D, Maddocks O, Otten G, Ratcliff M, Smith G, et al. Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity. elife. 2016;5: pubmed publisher
immunocytochemistry; human; 1:100	Denton K, Xu C, Li X. Modeling Axonal Phenotypes with Human Pluripotent Stem Cells. Methods Mol Biol. 2016;1353:309-21 pubmed publisher
immunocytochemistry; scFv; 1:100; fig 1	Ramaswamy K, Yik W, Wang X, Oliphant E, Lu W, Shibata D, et al. Derivation of induced pluripotent stem cells from orangutan skin fibroblasts. BMC Res Notes. 2015;8:577 pubmed publisher
flow cytometry; scFv; 1:400; loading ...; fig 1d	Lou Y, Kanninen L, Kaehr B, Townson J, Niklander J, HarjumÃ¤ki R, et al. Silica bioreplication preserves three-dimensional spheroid structures of human pluripotent stem cells and HepG2 cells. Sci Rep. 2015;5:13635 pubmed publisher
immunocytochemistry; scFv; 1:50; fig s8	Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, et al. An alternative pluripotent state confers interspecies chimaeric competency. Nature. 2015;521:316-21 pubmed publisher
immunocytochemistry; scFv; 1:300; fig 4	Chou B, Gu H, Gao Y, Dowey S, Wang Y, Shi J, et al. A facile method to establish human induced pluripotent stem cells from adult blood cells under feeder-free and xeno-free culture conditions: a clinically compliant approach. Stem Cells Transl Med. 2015;4:320-32 pubmed publisher
immunohistochemistry - frozen section; rat; 1:200	Schwartz C, Cheng A, Mughal M, Mattson M, Yao P. Clathrin assembly proteins AP180 and CALM in the embryonic rat brain. J Comp Neurol. 2010;518:3803-18 pubmed publisher
immunocytochemistry; scFv; 1:100; loading ...; fig 2c	Dattena M, Chessa B, Lacerenza D, Accardo C, Pilichi S, Mara L, et al. Isolation, culture, and characterization of embryonic cell lines from vitrified sheep blastocysts. Mol Reprod Dev. 2006;73:31-9 pubmed
	Schrobback K, Wrobel J, Hutmacher D, Woodfield T, Klein T. Stage-specific embryonic antigen-4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells. Tissue Eng Part A. 2013;19:1316-26 pubmed publisher
	Turnpenny L, Brickwood S, Spalluto C, Piper K, Cameron I, Wilson D, et al. Derivation of human embryonic germ cells: an alternative source of pluripotent stem cells. Stem Cells. 2003;21:598-609 pubmed
	Dodd J, Solter D, Jessell T. Monoclonal antibodies against carbohydrate differentiation antigens identify subsets of primary sensory neurones. Nature. 1984;311:469-72 pubmed
	Kannagi R, Cochran N, Ishigami F, Hakomori S, Andrews P, Knowles B, et al. Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J. 1983;2:2355-61 pubmed
	Tippett P, Andrews P, Knowles B, Solter D, Goodfellow P. Red cell antigens P (globoside) and Luke: identification by monoclonal antibodies defining the murine stage-specific embryonic antigens -3 and -4 (SSEA-3 and SSEA-4). Vox Sang. 1986;51:53-6 pubmed

product information

Internal ID :

2250

Name :

MC-813-70 (SSEA-4)

Depositor Name :

Solter, D. / Knowles, B.B

Depositor Institution :

A*STAR Institute of Medical Biology

Date Deposited :

6/11/87

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

SSEA-4/ stage-specific embryonic antigen 4/globolipid

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG3

Isotype for catalog (legacy) :

IgG3, kappa light chain

Positive Tested Species Reactivity :

Canine,Cat,Chicken,Equine,Human,Mouse,Non-human primates,Rabbit,Rat

Species Tested (legacy) :

see references

Initial Publication Pubmed ID :

6141938

Depositor Notes (Special Instructions) :

This antibody recognizes a defined carbohydrate epitope on a glycolipid expressed by human embryonic carcinoma and embryonic stem cells.

Collections :

Cell markers,Human,Stem cell

Search Keywords :

Solter, D. / Knowles, B.B, SSEA-4/ stage-specific embryonic antigen 4/globolipid, Human, MIgG3, Human/Mouse/Rat/Cat/Non-human primates/Equine/Canine/Chicken/Rabbit, , , AB_528477, Monoclonal, Human, Immunohistochemistry/Immunofluorescence/FACS/ELISA/Western blot

Antibody Registry ID :

AB_528477

Immunogen :

Human embryonal carcinoma 2102Ep cells

Immunogen Sequence :

Intact cells

Clonality :

Monoclonal

Myeloma Strain :

SP2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

Carbohydrate moiety of glycolipid/ NeuAc alpha2- 3Gal beta1- 3GalNAc beta1

Epitope Map PubMed ID :

6141938

Recommended Applications :

ELISA,FACS,Immunofluorescence,Immunohistochemistry,Western Blot

Immunohistochemistry Pubmed IDs :

6207435 12968114

Immunofluorescence Pubmed IDs :

6141938 6207435 27694817 29670257

FACS Pubmed IDs :

2874662 23301556

Additional Information :

RRID:AB_528477

DSHB Growth Medium :

Iscove's

References (legacy) :

EMBO J. 2, 2355-2361.; Nature 311, 469-472.; Vox Sang 51, 53-56.; "Principles of Management of Testicular Cancer", (N. Javadpour, ed.), pp. 88-98. Thieme, Inc., New York.; Stem Cells 21, 598-609.; Curr. Top. Microbiol. Immunol. 280, 71-109.; Anat. Rec. Part A 276A, 75-102.; EMBO Mol. Med. 4(5), 380-395.; Genesis 52, 49-55.

more info or order :

Developmental Studies Hybridoma Bank product webpage