Stage-specific embryonic antigen 1/CD15 | Developmental Studies Hybridoma Bank MC-480 (SSEA-1) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Stage-specific embryonic antigen 1/CD15

catalog :

MC-480 (SSEA-1)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MC-480 (SSEA-1)

reactivity :

human, mouse, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS, immunohistochemistry - paraffin section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 49

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:100	Pino Barrio M, GarcÃa GarcÃa E, MenÃ©ndez P, MartÃnez Serrano A. V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits. PLoS ONE. 2015;10:e0118499 pubmed publisher
immunocytochemistry; scFv; 1:100	Liskovykh M, Ponomartsev S, Popova E, Bader M, Kouprina N, Larionov V, et al. Stable maintenance of de novo assembled human artificial chromosomes in embryonic stem cells and their differentiated progeny in mice. Cell Cycle. 2015;14:1268-73 pubmed publisher
immunohistochemistry - paraffin section; scFv	Geula S, Moshitch Moshkovitz S, Dominissini D, Mansour A, Kol N, Salmon Divon M, et al. Stem cells. m6A mRNA methylation facilitates resolution of naÃ¯ve pluripotency toward differentiation. Science. 2015;347:1002-6 pubmed publisher
flow cytometry; scFv; 1:400; fig 5	Bittencourt D, Lee B, Gao L, Gerke D, Stallcup M. Role of distinct surfaces of the G9a ankyrin repeat domain in histone and DNA methylation during embryonic stem cell self-renewal and differentiation. Epigenetics Chromatin. 2014;7:27 pubmed publisher
flow cytometry; chicken immunocytochemistry; chicken	Lu Y, West F, Jordan B, Jordan E, West R, Yu P, et al. Induced pluripotency in chicken embryonic fibroblast results in a germ cell fate. Stem Cells Dev. 2014;23:1755-64 pubmed publisher
	Yuan Y, Yang Y, Tian Y, Park J, Dai A, Roberts R, et al. Efficient long-term cryopreservation of pluripotent stem cells at -80?°C. Sci Rep. 2016;6:34476 pubmed publisher
	Richardson R, Hammond N, Coulombe P, Saloranta C, Nousiainen H, Salonen R, et al. Periderm prevents pathological epithelial adhesions during embryogenesis. J Clin Invest. 2014;124:3891-900 pubmed publisher
	Lou Y, Wang P, Yeh S, Chuang P, Li S, Wu C, et al. Stage-specific embryonic antigen-4 as a potential therapeutic target in glioblastoma multiforme and other cancers. Proc Natl Acad Sci U S A. 2014;111:2482-7 pubmed publisher
	Miyazaki T, Nakatsuji N, Suemori H. Optimization of slow cooling cryopreservation for human pluripotent stem cells. Genesis. 2014;52:49-55 pubmed publisher
	Chan W, Sideris A, Sutachan J, Montoya G J, Blanck T, Recio Pinto E. Differential regulation of proliferation and neuronal differentiation in adult rat spinal cord neural stem/progenitors by ERK1/2, Akt, and PLC?. Front Mol Neurosci. 2013;6:23 pubmed publisher
	Schrobback K, Wrobel J, Hutmacher D, Woodfield T, Klein T. Stage-specific embryonic antigen-4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells. Tissue Eng Part A. 2013;19:1316-26 pubmed publisher
	Vedadi M, Barsyte Lovejoy D, Liu F, Rival Gervier S, Allali Hassani A, Labrie V, et al. A chemical probe selectively inhibits G9a and GLP methyltransferase activity in cells. Nat Chem Biol. 2011;7:566-74 pubmed publisher
	Tsukiyama T, Asano R, Kawaguchi T, Kim N, Yamada M, Minami N, et al. Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system. Genes Cells. 2011;16:815-25 pubmed publisher
	Gaspar Maia A, Alajem A, Polesso F, Sridharan R, Mason M, Heidersbach A, et al. Chd1 regulates open chromatin and pluripotency of embryonic stem cells. Nature. 2009;460:863-8 pubmed publisher
	Hermann A, Suess C, Fauser M, Kanzler S, Witt M, Fabel K, et al. Rostro-caudal gradual loss of cellular diversity within the periventricular regions of the ventricular system. Stem Cells. 2009;27:928-41 pubmed publisher
	Matusek T, Gombos R, Szécsényi A, Sanchez Soriano N, Czibula A, Pataki C, et al. Formin proteins of the DAAM subfamily play a role during axon growth. J Neurosci. 2008;28:13310-9 pubmed publisher
	Chen H, Kuo H, Chien C, Shun C, Yao Y, Ip P, et al. Derivation, characterization and differentiation of human embryonic stem cells: comparing serum-containing versus serum-free media and evidence of germ cell differentiation. Hum Reprod. 2007;22:567-77 pubmed
	Escrevente C, Machado E, Brito C, Reis C, Stoeck A, Runz S, et al. Different expression levels of alpha3/4 fucosyltransferases and Lewis determinants in ovarian carcinoma tissues and cell lines. Int J Oncol. 2006;29:557-66 pubmed
	Greenlee A, Kronenwetter Koepel T, Kaiser S, Liu K. Comparison of Matrigel and gelatin substrata for feeder-free culture of undifferentiated mouse embryonic stem cells for toxicity testing. Toxicol In Vitro. 2005;19:389-97 pubmed
	Shen Y, Mani S, Meiri K. Failure to express GAP-43 leads to disruption of a multipotent precursor and inhibits astrocyte differentiation. Mol Cell Neurosci. 2004;26:390-405 pubmed
	Greenlee A, Kronenwetter Koepel T, Kaiser S, Ellis T, Liu K. Combined effects of Matrigel and growth factors on maintaining undifferentiated murine embryonic stem cells for embryotoxicity testing. Toxicol In Vitro. 2004;18:543-53 pubmed
	Rosler E, Fisk G, Ares X, Irving J, Miura T, Rao M, et al. Long-term culture of human embryonic stem cells in feeder-free conditions. Dev Dyn. 2004;229:259-74 pubmed
	Carpenter M, Rosler E, Fisk G, Brandenberger R, Ares X, Miura T, et al. Properties of four human embryonic stem cell lines maintained in a feeder-free culture system. Dev Dyn. 2004;229:243-58 pubmed
	Young H, Black A. Adult stem cells. Anat Rec A Discov Mol Cell Evol Biol. 2004;276:75-102 pubmed
	Young H. Existence of reserve quiescent stem cells in adults, from amphibians to humans. Curr Top Microbiol Immunol. 2004;280:71-109 pubmed
	Turnpenny L, Brickwood S, Spalluto C, Piper K, Cameron I, Wilson D, et al. Derivation of human embryonic germ cells: an alternative source of pluripotent stem cells. Stem Cells. 2003;21:598-609 pubmed
	Buehr M, Nichols J, Stenhouse F, Mountford P, Greenhalgh C, Kantachuvesiri S, et al. Rapid loss of Oct-4 and pluripotency in cultured rodent blastocysts and derivative cell lines. Biol Reprod. 2003;68:222-9 pubmed
	Chang D, Cattoretti G, Calame K. The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development. Mech Dev. 2002;117:305-9 pubmed
	Jiang Y, Jahagirdar B, Reinhardt R, Schwartz R, Keene C, Ortiz Gonzalez X, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature. 2002;418:41-9 pubmed
	Bjorklund L, Sanchez Pernaute R, Chung S, Andersson T, Chen I, McNaught K, et al. Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model. Proc Natl Acad Sci U S A. 2002;99:2344-9 pubmed
	Kanungo J, Pratt S, Marie H, Longmore G. Ajuba, a cytosolic LIM protein, shuttles into the nucleus and affects embryonal cell proliferation and fate decisions. Mol Biol Cell. 2000;11:3299-313 pubmed
	Pfendler K, Yoon J, Taborn G, Kuehn M, Iannaccone P. Nodal and bone morphogenetic protein 5 interact in murine mesoderm formation and implantation. Genesis. 2000;28:1-14 pubmed
	Shamblott M, Axelman J, Wang S, Bugg E, Littlefield J, Donovan P, et al. Derivation of pluripotent stem cells from cultured human primordial germ cells. Proc Natl Acad Sci U S A. 1998;95:13726-31 pubmed
	Jho E, Davis R, Malbon C. c-Jun amino-terminal kinase is regulated by Galpha12/Galpha13 and obligate for differentiation of P19 embryonal carcinoma cells by retinoic acid. J Biol Chem. 1997;272:24468-74 pubmed
	DasGupta S, Hogan E, Spicer S. Stage-specific expression of fuco-neolacto- (Lewis X) and ganglio-series neutral glycosphingolipids during brain development: characterization of Lewis X and related glycosphingolipids in bovine, human and rat brain. Glycoconj J. 1996;13:367-75 pubmed
	Pain B, Clark M, Shen M, Nakazawa H, Sakurai M, Samarut J, et al. Long-term in vitro culture and characterisation of avian embryonic stem cells with multiple morphogenetic potentialities. Development. 1996;122:2339-48 pubmed
	Fox N, Damjanov I, Knowles B, Solter D. Teratocarcinoma antigen is secreted by epididymal cells and coupled to maturing sperm. Exp Cell Res. 1982;137:485-8 pubmed
	Knowles B, Pan S, Solter D, Linnenbach A, Croce C, Huebner K. Expression of H-2, laminin and SV40 T and TASA on differentiation of transformed murine teratocarcinoma cells. Nature. 1980;288:615-8 pubmed
	Knowles B, Rappaport J, Solter D. Murine embryonic antigen (SSEA-1) is expressed on human cells and structurally related human blood group antigen I is expressed on mouse embryos. Dev Biol. 1982;93:54-8 pubmed
	Gooi H, Feizi T, Kapadia A, Knowles B, Solter D, Evans M. Stage-specific embryonic antigen involves alpha 1 goes to 3 fucosylated type 2 blood group chains. Nature. 1981;292:156-8 pubmed
	Damjanov I, Fox N, Knowles B, Solter D, Lange P, Fraley E. Immunohistochemical localization of murine stage-specific embryonic antigens in human testicular germ cell tumors. Am J Pathol. 1982;108:225-30 pubmed
	Fox N, Damjanov I, Knowles B, Solter D. Immunohistochemical localization of the mouse stage-specific embryonic antigen 1 in human tissues and tumors. Cancer Res. 1983;43:669-78 pubmed
	Lagenaur C, Schachner M, Solter D, Knowles B. Monoclonal antibody against SSEA-1 is specific for a subpopulation of astrocytes in mouse cerebellum. Neurosci Lett. 1982;31:181-4 pubmed
	Fox N, Shevinsky L, Knowles B, Solter D, Dawjanov I. Distribution of murine stage-specific embryonic antigens in the kidneys of three rodent species. Exp Cell Res. 1982;140:331-9 pubmed
	Hakomori S, Nudelman E, Levery S, Solter D, Knowles B. The hapten structure of a developmentally regulated glycolipid antigen (SSEA-1) isolated from human erythrocytes and adenocarcinoma: a preliminary note. Biochem Biophys Res Commun. 1981;100:1578-86 pubmed
	Fox N, Damjanov I, Martinez Hernandez A, Knowles B, Solter D. Immunohistochemical localization of the early embryonic antigen (SSEA-1) in postimplantation mouse embryos and fetal and adult tissues. Dev Biol. 1981;83:391-8 pubmed
	Ozawa M, Muramatsu T, Solter D. SSEA-1, a stage-specific embryonic antigen of the mouse, is carried by the glycoprotein-bound large carbohydrate in embryonal carcinoma cells. Cell Differ. 1985;16:169-73 pubmed
	Whitesell L, Rosolen A, Neckers L. Episome-generated N-myc antisense RNA restricts the differentiation potential of primitive neuroectodermal cell lines. Mol Cell Biol. 1991;11:1360-71 pubmed
	Ballou B, Levine G, Hakala T, Solter D. Tumor location detected with radioactively labeled monoclonal antibody and external scintigraphy. Science. 1979;206:844-7 pubmed

product information

Internal ID :

2240

Name :

MC-480 (SSEA-1)

Depositor Name :

Solter, D. / Knowles, B.B

Depositor Institution :

Institute of Medical Biology, Singapore

Date Deposited :

6/11/87

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

Stage-specific embryonic antigen 1/CD15

Antigen Species :

Mouse

Host Species :

mouse

Isotype :

MIgM, kappa light chain

Isotype for catalog (legacy) :

IgM, kappa light chain

Positive Tested Species Reactivity :

Bovine,Chicken,Drosophila,Equine,Human,Mouse,Primate,Rat

Species Tested (legacy) :

MC-480 recognizes mouse embryos, mouse embryonic cells (EC), mouse embryonic stem cells (ES) and mouse & human embryonic germ cells (EG). MC-480 does not bind to human EC, ES or iPS cells.

Initial Publication Pubmed ID :

281705

Depositor Notes (Special Instructions) :

MC-480 recognizes mouse embryos, mouse embryonic cells (EC), mouse embryonic stem cells (ES) and mouse & human embryonic germ cells (EG). MC-480 does not bind to human EC, ES or iPS cells.

Collections :

CD antigens,Cell adhesion,Cell markers,Drosophila antigens,Human

Search Keywords :

Solter, D. / Knowles, B.B, SSEA-1/ alpha-(1,3)-fucosyltransferase / CD15 , Mouse, MIgM, kappa light chain, Human/Mouse/Rat/Bovine/Equine/Primate/Drosophila, Fut4, AI451562, CD15, FAL, FucT-IV, LeX, SSEA-1, AB_528475, Monoclonal

Antibody Registry ID :

AB_528475

Synonyms (Alternate Clone Names) :

anti-CD15, anti-Lewis X

Immunogen :

Irradiated testis embryonal carcinoma F9 cell line

Clonality :

Monoclonal

Myeloma Strain :

P3-X63-Ag8

Epitope Mapped :

Yes

Epitope Location or Sequence :

SSEA-1 is a carbohydrate epitope on glycolipids and glycoproteins involving fucosylated [GalBeta1-4(FucAlpha1-3)GlcNAcBeta1-R] type 2 blood group chains.

Epitope Map PubMed ID :

6165896 6117281

Recommended Applications :

FACS,FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunohistochemistry Pubmed IDs :

21658168 19353521 9811868 6113181 6152898 6129058 8781967 11020711 12204275 12968114 14594208 14699636

Immunofluorescence Pubmed IDs :

23986655 11029037 9305908 19052223 19587682 16865271 89977 7035205 6126385 6152898 6127658 6129058 1996098 8756279 11782534 12493717 15130612 15234344 15713546 25133425 27694817

Western Blot Pubmed IDs :

16865271

Immunoprecipitation Pubmed IDs :

2860973

FFPE Pubmed IDs :

19587682 24550271

FACS Pubmed IDs :

14745950 14745951 21743462 16865271 12077603 23301556

Pubmed IDs :

493985 6255342 6182045 17071820 24254533

Additional Information :

For experiments involving live cells and tissue, MC-480 supernatant is available without the antimicrobial ProClin. Contact us at 319-335-3826 or dshb@uiowa.edu for special ordering instructions. MC-480 has been successfully used for TLC Overlay applications (PMID: 26405105).

DSHB Growth Medium :

Iscove's

References (legacy) :

Natl. Acad. Sci. USA 75, 5565-5569. ; Dev. Biol. 70, 515-521.; Science 206, 844-847.; Nature 288, 615-618.; Dev. Biol. 83, 391-398.; Nature 292, 156-158.; Biochem. Biophys. Res. Comm. 100, 1578-1586.; Exp. Cell Res. 137, 485-488.; Exp. Cell. Res. 140, 331-339.; Am. J. Pathol. 108, 225-230.; Dev. Biol. 93, 54-58.; Neurosci. Letters 31, 181-184.; Cancer Res. 43, 669-678.; Cell Differentiation 16, 169-173.; In “Principles and Management of Testicular Cancer” (N. Javadpour, ed.), pp. 88-98. Thieme, Inc., New York.; Mol. Cell Bio. 11(3), 1360-1371.; Glycoconjugate J. 13, 367-375.; Development 122, 2339-2348.; Genesis 28, 1-14.; Proc. Natl. Acad. Sci. USA 99(4), 2344-2349.; Nature 418, 41-49.; Mech. Dev. 117(1-2), 305-309.; Stem Cells 21, 598-609.; Biol. Reprod. 68, 222-229.; Curr. Top. Microbiol. Immunol. 280, 71-109.; Anat. Rec. Part A 276A, 75-102.; Toxicol. Vitr. 18, 543-553.; Mol. Cell. Neurosci. 26, 390-405.; Toxicol. Vitr. 19, 389-397.; Hum. Reprod. 22(2), 567-577.; Genesis 52, 49-55.; J. Clin. Invest. doi:10.1172/JCI71946.

more info or order :

Developmental Studies Hybridoma Bank product webpage