TRP protein (transient receptor potential) | Developmental Studies Hybridoma Bank MAb83F6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

TRP protein (transient receptor potential)

catalog :

MAb83F6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MAb83F6

reactivity :

fruit fly

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 12

Reference
Voolstra O, Rhodes Mordov E, Katz B, Bartels J, Oberegelsbacher C, Schotthöfer S, et al. The Phosphorylation State of the Drosophila TRP Channel Modulates the Frequency Response to Oscillating Light In Vivo. J Neurosci. 2017;37:4213-4224 pubmed publisher
Cerny A, Altendorfer A, Schopf K, Baltner K, Maag N, Sehn E, et al. The GTP- and Phospholipid-Binding Protein TTD14 Regulates Trafficking of the TRPL Ion Channel in Drosophila Photoreceptor Cells. PLoS Genet. 2015;11:e1005578 pubmed publisher
Voolstra O, Bartels J, Oberegelsbacher C, Pfannstiel J, Huber A. Phosphorylation of the Drosophila transient receptor potential ion channel is regulated by the phototransduction cascade and involves several protein kinases and phosphatases. PLoS ONE. 2013;8:e73787 pubmed publisher
Katz B, Oberacker T, Richter D, Tzadok H, Peters M, Minke B, et al. Drosophila TRP and TRPL are assembled as homomultimeric channels in vivo. J Cell Sci. 2013;126:3121-33 pubmed publisher
Richter D, Katz B, Oberacker T, Tzarfaty V, Belusic G, Minke B, et al. Translocation of the Drosophila transient receptor potential-like (TRPL) channel requires both the N- and C-terminal regions together with sustained Ca2+ entry. J Biol Chem. 2011;286:34234-43 pubmed publisher
Sanxaridis P, Tsunoda S. A forward genetic screen in Drosophila melanogaster to identify mutations affecting INAD localization in photoreceptor cells. Fly (Austin). 2010;4:95-103 pubmed
Voolstra O, Beck K, Oberegelsbacher C, Pfannstiel J, Huber A. Light-dependent phosphorylation of the drosophila transient receptor potential ion channel. J Biol Chem. 2010;285:14275-84 pubmed publisher
Parnas M, Katz B, Lev S, Tzarfaty V, Dadon D, Gordon Shaag A, et al. Membrane lipid modulations remove divalent open channel block from TRP-like and NMDA channels. J Neurosci. 2009;29:2371-83 pubmed publisher
Meyer N, Oberegelsbacher C, Dürr T, Schäfer A, Huber A. An eGFP-based genetic screen for defects in light-triggered subcelluar translocation of the Drosophila photoreceptor channel TRPL. Fly (Austin). 2008;2:36-46 pubmed
Cheng Y, Nash H. Drosophila TRP channels require a protein with a distinctive motif encoded by the inaF locus. Proc Natl Acad Sci U S A. 2007;104:17730-4 pubmed
Gillo B, Chorna I, Cohen H, Cook B, Manistersky I, Chorev M, et al. Coexpression of Drosophila TRP and TRP-like proteins in Xenopus oocytes reconstitutes capacitative Ca2+ entry. Proc Natl Acad Sci U S A. 1996;93:14146-51 pubmed
Pollock J, Assaf A, Peretz A, Nichols C, Mojet M, Hardie R, et al. TRP, a protein essential for inositide-mediated Ca2+ influx is localized adjacent to the calcium stores in Drosophila photoreceptors. J Neurosci. 1995;15:3747-60 pubmed

product information

Internal ID :

921

Name :

MAb83F6

Depositor Name :

Benzer, S.

Depositor Institution :

California Institute of Technology

Date Deposited :

7/27/05

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

TRP protein (transient receptor potential)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

7751943

Depositor Notes (Special Instructions) :

Overfixation reduces the efficacy of MAb83F6

Collections :

Cell signaling,Drosophila antigens,Neurodevelopment,Skeletal muscle

Search Keywords :

Benzer, S., TRP protein (transient receptor potential), Drosophila, MIgG1, kappa light chain, Drosophila, trp, CG7875, 559, AB_528496, monoclonal, Cell signaling/Drosophila antigens/Skeletal muscle/Neurodevelopment, Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 143 kDa; Apparent: 143 kDa

Gene :

trp

Alternate Gene Name(s) :

CG7875, 559

Uniprot ID :

P19334

Antibody Registry ID :

AB_528496

Immunogen :

Purified rhabdomeres from Drosophila eyes.

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Epitope Mapped :

Yes

Epitope Location or Sequence :

Intracellular C-terminal (aa 675-1275)

Epitope Map PubMed ID :

23687378

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

7751943 20215118 17968007 23687378 24040070 26509977

Western Blot Pubmed IDs :

7751943 8943075 19244513 20215118 17968007 23687378 21816824 18820462 20404479 26509977

Immunoprecipitation Pubmed IDs :

20215118 23687378

Additional Information :

Doesn't stain null trp fly mutants. trp is a transmembrane light-sensitive calcium channel required for phototransduction.

DSHB Growth Medium :

RPMI

References (legacy) :

J. Neurosci. 15(5), 3747-3760.; Proc. Natl. Acad. Sci. USA 93(24), 14146-14151.; J. Neurosci. 29(8), 2371-2383.

more info or order :

Developmental Studies Hybridoma Bank product webpage