SMN1 protein, exon 5 | Developmental Studies Hybridoma Bank MANSMA3(8E1) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

SMN1 protein, exon 5

catalog :

MANSMA3(8E1)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MANSMA3(8E1)

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 2

Reference
Young P, Man N, Lorson C, Le T, Androphy E, Burghes A, et al. The exon 2b region of the spinal muscular atrophy protein, SMN, is involved in self-association and SIP1 binding. Hum Mol Genet. 2000;9:2869-77 pubmed
Young P, Le T, thi Man N, Burghes A, Morris G. The relationship between SMN, the spinal muscular atrophy protein, and nuclear coiled bodies in differentiated tissues and cultured cells. Exp Cell Res. 2000;256:365-74 pubmed

product information

Internal ID :

7304

Name :

MANSMA3(8E1)

Depositor Name :

Morris, G.E.

Depositor Institution :

Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, UK

Date Deposited :

6/13/11

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

SMN1 protein, exon 5

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human,Mouse

Species Tested (legacy) :

human, mouse (weak), not rabbit

Initial Publication Pubmed ID :

10772809

Depositor Notes (Special Instructions) :

Stains SMN band in HeLa total protein extract (with 1 higher Mr band). Stains dot-like structures (gems) in the nucleus. The staining of mouse SMN is weak. Doesn'r work on rabbit.

Collections :

Human,Muscular dystrophy,Skeletal muscle

Search Keywords :

Morris, G.E., SMN1 protein, exon 5, Human, MIgG1, Human/Mouse, SMN1, SMN, SMNT, SMN2, SMNC, Gemin1, AB_2618241, monoclonal, Human/Muscular dystrophy/Skeletal muscle, Immunofluorescence/Western Blot/ELISA

Antigen Molecular Weight :

Predicted: 32 kDa; Apparent: 38 kDa

Gene :

SMN1

Alternate Gene Name(s) :

SMN, SMNT, SMN2, SMNC, Gemin1

Uniprot ID :

Q16637

Antibody Registry ID :

AB_2618241

Synonyms (Alternate Clone Names) :

MANSMA3

Immunogen :

Recombinant SMN protein

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

Sp2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

exon 5, aa 210-241 of full length isoform SMN

Epitope Map PubMed ID :

10772809 11092763

Recommended Applications :

ELISA,Immunofluorescence,Western Blot

Immunoblotting (legacy) :

yes stains a 38 kDa SMN band

Immunofluorescence Pubmed IDs :

10772809

Western Blot Pubmed IDs :

10772809

ELISA Pubmed IDs :

10772809

Additional Information :

Contrary to what was published in the initial publication, the Depositor stated that this antibody doesn't work on rabbit (see Depositor notes).

Additional Characterization :

http://www.glennmorris.org.uk/datasheets/MANSMA3(8E1).doc

DSHB Growth Medium :

Iscove's

References (legacy) :

Exp. Cell Res. 256, 365-374.

more info or order :

Developmental Studies Hybridoma Bank product webpage