SMN1 protein, exon 2 | Developmental Studies Hybridoma Bank MANSMA2(8F7) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

SMN1 protein, exon 2

catalog :

MANSMA2(8F7)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MANSMA2(8F7)

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 5

Reference
Mack S, Cook D, Dhurjati P, Butchbach M. Systems biology investigation of cAMP modulation to increase SMN levels for the treatment of spinal muscular atrophy. PLoS ONE. 2014;9:e115473 pubmed publisher
Foust K, Wang X, McGovern V, Braun L, Bevan A, Haidet A, et al. Rescue of the spinal muscular atrophy phenotype in a mouse model by early postnatal delivery of SMN. Nat Biotechnol. 2010;28:271-4 pubmed publisher
Thurmond J, Butchbach M, Palomo M, Pease B, Rao M, Bedell L, et al. Synthesis and biological evaluation of novel 2,4-diaminoquinazoline derivatives as SMN2 promoter activators for the potential treatment of spinal muscular atrophy. J Med Chem. 2008;51:449-69 pubmed publisher
Young P, Man N, Lorson C, Le T, Androphy E, Burghes A, et al. The exon 2b region of the spinal muscular atrophy protein, SMN, is involved in self-association and SIP1 binding. Hum Mol Genet. 2000;9:2869-77 pubmed
Young P, Le T, thi Man N, Burghes A, Morris G. The relationship between SMN, the spinal muscular atrophy protein, and nuclear coiled bodies in differentiated tissues and cultured cells. Exp Cell Res. 2000;256:365-74 pubmed

product information

Internal ID :

7340

Name :

MANSMA2(8F7)

Depositor Name :

Morris, G.E.

Depositor Institution :

Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, UK

Date Deposited :

6/13/11

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

SMN1 protein, exon 2

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human,Mouse,Rabbit

Species Tested (legacy) :

human, mouse (weak), rabbit

Initial Publication Pubmed ID :

10772809

Depositor Notes (Special Instructions) :

Stains only SMN band in HeLa total protein extract. Stains dot-like structures (gems) in the nucleus. The staining of mouse SMN is weak.

Collections :

Human,Muscular dystrophy,Skeletal muscle

Search Keywords :

Morris, G.E., SMN1 protein, exon 2, Human, MIgG1, Human/Rabbit/Mouse, SMN1, SMN, SMNT, SMN2, SMNC, Gemin1, AB_2618238, monoclonal, Human/Muscular dystrophy/Skeletal muscle, Immunofluorescence/Western Blot/ELISA

Antigen Molecular Weight :

Predicted: 32 kDa; Apparent: 38 kDa

Gene :

SMN1

Alternate Gene Name(s) :

SMN, SMNT, SMN2, SMNC, Gemin1

Uniprot ID :

Q16637

Antibody Registry ID :

AB_2618238

Synonyms (Alternate Clone Names) :

MANSMA2

Immunogen :

Recombinant SMN protein

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

Sp2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

exon 2, aa 28-91

Epitope Map PubMed ID :

11092763

Recommended Applications :

ELISA,Immunofluorescence,Western Blot

Immunoblotting (legacy) :

yes stains a 38 kDa SMN band

Immunofluorescence Pubmed IDs :

10772809 18205293

Western Blot Pubmed IDs :

10772809 20190738

ELISA Pubmed IDs :

10772809

Additional Characterization :

http://www.glennmorris.org.uk/datasheets/MANSMA2(8F7).doc

DSHB Growth Medium :

Iscove's

References (legacy) :

Exp. Cell Res. 256, 365-374.

more info or order :

Developmental Studies Hybridoma Bank product webpage