SMN1 protein, exon 2 | Developmental Studies Hybridoma Bank MANSMA1(11F3) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

SMN1 protein, exon 2

catalog :

MANSMA1(11F3)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MANSMA1(11F3)

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:400; loading ...; fig 1C western blot; human; loading ...; fig 1B	Yu Y, Chi B, Xia W, Gangopadhyay J, Yamazaki T, Winkelbauer Hurt M, et al. U1 snRNP is mislocalized in ALS patient fibroblasts bearing NLS mutations in FUS and is required for motor neuron outgrowth in zebrafish. Nucleic Acids Res. 2015;43:3208-18 pubmed publisher
	Custer S, Gilson T, Li H, Todd A, Astroski J, Lin H, et al. Altered mRNA Splicing in SMN-Depleted Motor Neuron-Like Cells. PLoS ONE. 2016;11:e0163954 pubmed publisher
	Custer S, Todd A, Singh N, Androphy E. Dilysine motifs in exon 2b of SMN protein mediate binding to the COPI vesicle protein ?-COP and neurite outgrowth in a cell culture model of spinal muscular atrophy. Hum Mol Genet. 2013;22:4043-52 pubmed publisher
	Francis J, Figueiredo D, vanderSpek J, Ayala L, Kim Y, Remington M, et al. A survival motor neuron:tetanus toxin fragment C fusion protein for the targeted delivery of SMN protein to neurons. Brain Res. 2004;995:84-96 pubmed
	Wehner K, Ayala L, Kim Y, Young P, Hosler B, Lorson C, et al. Survival motor neuron protein in the nucleolus of mammalian neurons. Brain Res. 2002;945:160-73 pubmed
	Young P, Man N, Lorson C, Le T, Androphy E, Burghes A, et al. The exon 2b region of the spinal muscular atrophy protein, SMN, is involved in self-association and SIP1 binding. Hum Mol Genet. 2000;9:2869-77 pubmed
	Young P, Le T, thi Man N, Burghes A, Morris G. The relationship between SMN, the spinal muscular atrophy protein, and nuclear coiled bodies in differentiated tissues and cultured cells. Exp Cell Res. 2000;256:365-74 pubmed

product information

Internal ID :

7300

Name :

MANSMA1(11F3)

Depositor Name :

Morris, G.E.

Depositor Institution :

Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, UK

Date Deposited :

6/13/11

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

SMN1 protein, exon 2

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human,Mouse,Porcine,Rat

Species Tested (legacy) :

human, mouse (weak), not rabbit

Initial Publication Pubmed ID :

10772809

Depositor Notes (Special Instructions) :

Stains SMN band in HeLa total protein extract (with 2 higher Mr bands). Stains dot-like structures (gems) in the nucleus. The staining of mouse SMN is weak. Doesn'r work on rabbit.

Collections :

Human,Muscular dystrophy,Skeletal muscle

Search Keywords :

Morris, G.E., SMN1 protein, exon 2, Human, MIgG1, Human/Porcine/Mouse/Rat, SMN1, SMN, SMNT, SMN2, SMNC, Gemin1, AB_2618227, monoclonal, Human/Muscular dystrophy/Skeletal muscle, Immunofluorescence/Western Blot/ELISA/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 32 kDa; Apparent: 38 kDa

Gene :

SMN1

Alternate Gene Name(s) :

SMN, SMNT, SMN2, SMNC, Gemin1

Uniprot ID :

Q16637

Antibody Registry ID :

AB_2618227

Synonyms (Alternate Clone Names) :

MANSMA1

Immunogen :

Recombinant SMN protein

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

Sp2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

exon 2, aa 28-91 (conformational epitope)

Epitope Map PubMed ID :

11092763

Recommended Applications :

ELISA,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes, stains a 38 kDa SMN band

Immunofluorescence Pubmed IDs :

10772809 14644474 12126878

Western Blot Pubmed IDs :

10772809 23727837 12126878 27736905

Immunoprecipitation Pubmed IDs :

11092763

ELISA Pubmed IDs :

10772809

Additional Information :

Contrary to what was published in the initial publication, the Depositor stated that this antibody doesn't work on rabbit (see Depositor notes).

Additional Characterization :

http://www.glennmorris.org.uk/datasheets/MANSMA1(11F3).doc

DSHB Growth Medium :

Iscove's

References (legacy) :

Exp. Cell Res. 256, 365-374.

more info or order :

Developmental Studies Hybridoma Bank product webpage