Dystrophin, exons 31/32 | Developmental Studies Hybridoma Bank MANDYS8(8H11) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Dystrophin, exons 31/32

catalog :

MANDYS8(8H11)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MANDYS8(8H11)

reactivity :

human, mouse, dog

application :

western blot, ELISA, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 9

Reference
Kodippili K, Vince L, Shin J, Yue Y, Morris G, McIntosh M, et al. Characterization of 65 epitope-specific dystrophin monoclonal antibodies in canine and murine models of duchenne muscular dystrophy by immunostaining and western blot. PLoS ONE. 2014;9:e88280 pubmed publisher
Morris G, Man N, Sewry C. Monitoring duchenne muscular dystrophy gene therapy with epitope-specific monoclonal antibodies. Methods Mol Biol. 2011;709:39-61 pubmed publisher
Romero N, Braun S, Benveniste O, Leturcq F, Hogrel J, Morris G, et al. Phase I study of dystrophin plasmid-based gene therapy in Duchenne/Becker muscular dystrophy. Hum Gene Ther. 2004;15:1065-76 pubmed
Bertoni C, Morris G, Rando T. Strand bias in oligonucleotide-mediated dystrophin gene editing. Hum Mol Genet. 2005;14:221-33 pubmed
Lu Q, Morris G, Wilton S, Ly T, Artem yeva O, Strong P, et al. Massive idiosyncratic exon skipping corrects the nonsense mutation in dystrophic mouse muscle and produces functional revertant fibers by clonal expansion. J Cell Biol. 2000;148:985-96 pubmed
Nguyen T, Morris G. Use of epitope libraries to identify exon-specific monoclonal antibodies for characterization of altered dystrophins in muscular dystrophy. Am J Hum Genet. 1993;52:1057-66 pubmed
Mendell J, Kissel J, Amato A, King W, Signore L, Prior T, et al. Myoblast transfer in the treatment of Duchenne's muscular dystrophy. N Engl J Med. 1995;333:832-8 pubmed
Sedgwick S, Nguyen T, Ellis J, Crowne H, Morris G. Rapid mapping by transposon mutagenesis of epitopes on the muscular dystrophy protein, dystrophin. Nucleic Acids Res. 1991;19:5889-94 pubmed
Nguyen thi Man -, Cartwright A, Morris G, Love D, Bloomfield J, Davies K. Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin. FEBS Lett. 1990;262:237-40 pubmed

product information

Internal ID :

8219

Name :

MANDYS8(8H11)

Depositor Name :

Morris, G.E.

Depositor Institution :

Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, UK

Date Deposited :

8/29/12

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Dystrophin, exons 31/32

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG2b

Isotype for catalog (legacy) :

IgG2b

Positive Tested Species Reactivity :

Canine,Human,Mouse

Species Tested (legacy) :

human, mouse (not xenopus)

Initial Publication Pubmed ID :

1692289

Depositor Notes (Special Instructions) :

Doesn't work on Xenopus. Fixation for immunostaining: acetone/methanol.

Collections :

Human,Muscular dystrophy,Skeletal muscle

Search Keywords :

Morris, G.E., Dystrophin, exons 31/32, Human, MIgG2b, Human/Mouse/Canine, DMD, BMD, CMD3B, MRX85, AB_2618170, monoclonal, Human/Muscular dystrophy/Skeletal muscle, Immunofluorescence/Western blot/ELISA

Antigen Molecular Weight :

Predicted: 427 kDa; Apparent: 427 kDa

Gene :

DMD

Alternate Gene Name(s) :

BMD, CMD3B, MRX85

Uniprot ID :

P11532

Antibody Registry ID :

AB_2618170

Immunogen :

Recombinant LacZ/dystrophin (aa 816-1749, rod domain of isoform 4 (Uniprot ID P11532-1)) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

Sp2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

exons 31/32, aa 1431-1505

Epitope Map PubMed ID :

7684887

Recommended Applications :

ELISA,Immunofluorescence,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

1692289 15610607 15563511 24516626 21194020 7651473 10704448

Western Blot Pubmed IDs :

1692289 15563511 24516626 21194020 1719482

ELISA Pubmed IDs :

1692289

Additional Information :

The collection of anti-DMD mAbs deposited by Morris, G.E., are directed against different DMD exons.

Additional Characterization :

http://www.glennmorris.org.uk/datasheets/MANDYS8(8H11).doc

DSHB Growth Medium :

Iscove's

References (legacy) :

FEBS Lett. 262, 237-240.; Am. J. Hum. Genet. 52, 1057-1066.

more info or order :

Developmental Studies Hybridoma Bank product webpage