Dystrophin, exon 21 | Developmental Studies Hybridoma Bank MANDYS19(8F6) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Dystrophin, exon 21

catalog :

MANDYS19(8F6)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MANDYS19(8F6)

reactivity :

human, mouse, dog, chicken

application :

western blot, ELISA, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; chicken; 1:5	Wahlin K, Moreira E, Huang H, Yu N, Adler R. Molecular dynamics of photoreceptor synapse formation in the developing chick retina. J Comp Neurol. 2008;506:822-37 pubmed
	MassouridÃ¨s E, Polentes J, Mangeot P, Mournetas V, Nectoux J, Deburgrave N, et al. Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells. Skelet Muscle. 2015;5:40 pubmed publisher
	Kodippili K, Vince L, Shin J, Yue Y, Morris G, McIntosh M, et al. Characterization of 65 epitope-specific dystrophin monoclonal antibodies in canine and murine models of duchenne muscular dystrophy by immunostaining and western blot. PLoS ONE. 2014;9:e88280 pubmed publisher
	Le T, Nguyen T, Morris G. Monoclonal antibodies for clinical trials of Duchenne muscular dystrophy therapy. Neuromuscul Disord. 2014;24:195-200 pubmed publisher
	Morris G, Man N, Sewry C. Monitoring duchenne muscular dystrophy gene therapy with epitope-specific monoclonal antibodies. Methods Mol Biol. 2011;709:39-61 pubmed publisher
	Lu Q, Morris G, Wilton S, Ly T, Artem yeva O, Strong P, et al. Massive idiosyncratic exon skipping corrects the nonsense mutation in dystrophic mouse muscle and produces functional revertant fibers by clonal expansion. J Cell Biol. 2000;148:985-96 pubmed
	Hori S, Ohtani S, Nguyen T, Morris G. The N-terminal half of dystrophin is protected from proteolysis in situ. Biochem Biophys Res Commun. 1995;209:1062-7 pubmed
	Nguyen T, Morris G. Use of epitope libraries to identify exon-specific monoclonal antibodies for characterization of altered dystrophins in muscular dystrophy. Am J Hum Genet. 1993;52:1057-66 pubmed
	Sedgwick S, Nguyen T, Ellis J, Crowne H, Morris G. Rapid mapping by transposon mutagenesis of epitopes on the muscular dystrophy protein, dystrophin. Nucleic Acids Res. 1991;19:5889-94 pubmed
	Nguyen thi Man -, Cartwright A, Morris G, Love D, Bloomfield J, Davies K. Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin. FEBS Lett. 1990;262:237-40 pubmed
	Nguyen T, Ginjaar I, van Ommen G, Morris G. Monoclonal antibodies for dystrophin analysis. Epitope mapping and improved binding to SDS-treated muscle sections. Biochem J. 1992;288 ( Pt 2):663-8 pubmed

product information

Internal ID :

3795

Name :

MANDYS19(8F6)

Depositor Name :

Morris, G.E.

Depositor Institution :

Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, UK

Date Deposited :

7/13/06

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Dystrophin, exon 21

Antigen Species :

human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Canine,Human,Mouse

Species Tested (legacy) :

human and mouse (not chick, torpedo)

Initial Publication Pubmed ID :

1692289

Depositor Notes (Special Instructions) :

This antibody does not work with chicken or torpedo

Collections :

Human,Muscular dystrophy,Skeletal muscle

Search Keywords :

Morris, dystrophin, , exon 21, MANDYS19, DMD, BMD, CMD3B, MRX85, monoclonal, human, AB_2618169, IgG1, Canine,Human,Mouse, ELISA,Immunofluorescence,Western Blot

Antigen Molecular Weight :

Predicted: 427 kDa; Apparent: 427 kDa

Gene :

DMD

Alternate Gene Name(s) :

BMD, CMD3B, MRX85

Uniprot ID :

P11532

Antibody Registry ID :

AB_2618169

Immunogen :

Recombinant LacZ/dystrophin (aa 816-1749, rod domain of isoform 4 (Uniprot ID P11532-1)) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

NS0 or Sp2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

exon 21. aa 901-904 (GQGP) of isoform 4 (Uniprot ID P11532-1)

Epitope Map PubMed ID :

7684887 24361045

Recommended Applications :

ELISA,Immunofluorescence,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

1692289 24516626 21194020 10704448

Western Blot Pubmed IDs :

1692289 24516626 21194020 1719482 26568816

ELISA Pubmed IDs :

1692289

Pubmed IDs :

7684887 1281410 7733959

Additional Characterization :

http://www.glennmorris.org.uk/datasheets/MANDYS19(8F6).doc

DSHB Growth Medium :

Iscove's

References (legacy) :

FEBS Lett. 262, 237-240.; Am. J. Hum. Genet. 52, 1057-1066.

more info or order :

Developmental Studies Hybridoma Bank product webpage