Dystrophin, exon 26 | Developmental Studies Hybridoma Bank MANDYS18(5H9) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Dystrophin, exon 26

catalog :

MANDYS18(5H9)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MANDYS18(5H9)

reactivity :

human, mouse, dog, chicken

application :

western blot, ELISA, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot; chicken; 1:5	Wahlin K, Moreira E, Huang H, Yu N, Adler R. Molecular dynamics of photoreceptor synapse formation in the developing chick retina. J Comp Neurol. 2008;506:822-37 pubmed
	Kodippili K, Vince L, Shin J, Yue Y, Morris G, McIntosh M, et al. Characterization of 65 epitope-specific dystrophin monoclonal antibodies in canine and murine models of duchenne muscular dystrophy by immunostaining and western blot. PLoS ONE. 2014;9:e88280 pubmed publisher
	Morris G, Man N, Sewry C. Monitoring duchenne muscular dystrophy gene therapy with epitope-specific monoclonal antibodies. Methods Mol Biol. 2011;709:39-61 pubmed publisher
	Lu Q, Morris G, Wilton S, Ly T, Artem yeva O, Strong P, et al. Massive idiosyncratic exon skipping corrects the nonsense mutation in dystrophic mouse muscle and produces functional revertant fibers by clonal expansion. J Cell Biol. 2000;148:985-96 pubmed
	Nguyen T, Morris G. Use of epitope libraries to identify exon-specific monoclonal antibodies for characterization of altered dystrophins in muscular dystrophy. Am J Hum Genet. 1993;52:1057-66 pubmed
	Sedgwick S, Nguyen T, Ellis J, Crowne H, Morris G. Rapid mapping by transposon mutagenesis of epitopes on the muscular dystrophy protein, dystrophin. Nucleic Acids Res. 1991;19:5889-94 pubmed
	Nguyen thi Man -, Cartwright A, Morris G, Love D, Bloomfield J, Davies K. Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin. FEBS Lett. 1990;262:237-40 pubmed
	Yarom R, Morris G, Froede R, Schaper J. Myocardial dystrophin immunolocalization at sarcolemma and transverse tubules. Experientia. 1992;48:614-6 pubmed

product information

Internal ID :

8283

Name :

MANDYS18(5H9)

Depositor Name :

Morris, G.E.

Depositor Institution :

Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, UK

Date Deposited :

11/14/12

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Dystrophin, exon 26

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG2a

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Canine,Human,Mouse

Species Tested (legacy) :

human, mouse

Initial Publication Pubmed ID :

1692289

Depositor Notes (Special Instructions) :

Fixation for immunostaining: acetone/methanol.

Collections :

Human,Muscular dystrophy,Skeletal muscle

Search Keywords :

Morris, G.E., Dystrophin, exon 26, Human, MIgG2a, Canine/Human/Mouse, DMD, BMD, CMD3B, MRX85, AB_2618168, monoclonal, Human/Muscular dystrophy/Skeletal muscle, Immunofluorescence/Western blot/ELISA

Antigen Molecular Weight :

Predicted: 427 kDa; Apparent: 427 kDa

Gene :

DMD

Alternate Gene Name(s) :

BMD, CMD3B, MRX85

Uniprot ID :

P11532

Antibody Registry ID :

AB_2618168

Immunogen :

Recombinant LacZ/dystrophin (aa 816-1749, rod domain of isoform 4 (Uniprot ID P11532-1)) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

Sp2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

exon 26, aa 1181-1187

Epitope Map PubMed ID :

7684887

Recommended Applications :

ELISA,Immunofluorescence,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

1692289 24516626 21194020 1612140 10704448

Western Blot Pubmed IDs :

1692289 24516626 21194020 1719482

ELISA Pubmed IDs :

1692289

Additional Information :

The collection of anti-DMD mAbs deposited by Morris, G.E., are directed against different DMD exons.

Additional Characterization :

http://www.glennmorris.org.uk/datasheets/MANDYS18(5H9).doc

DSHB Growth Medium :

Iscove's

References (legacy) :

FEBS Lett. 262, 237-240.; Am. J. Hum. Genet. 52, 1057-1066.

more info or order :

Developmental Studies Hybridoma Bank product webpage