Dystrophin, exon 26 | Developmental Studies Hybridoma Bank MANDYS17(3B12) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Dystrophin, exon 26

catalog :

MANDYS17(3B12)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MANDYS17(3B12)

reactivity :

human, mouse, dog, chicken

application :

western blot, ELISA, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot; chicken; 1:5	Wahlin K, Moreira E, Huang H, Yu N, Adler R. Molecular dynamics of photoreceptor synapse formation in the developing chick retina. J Comp Neurol. 2008;506:822-37 pubmed
	Kodippili K, Vince L, Shin J, Yue Y, Morris G, McIntosh M, et al. Characterization of 65 epitope-specific dystrophin monoclonal antibodies in canine and murine models of duchenne muscular dystrophy by immunostaining and western blot. PLoS ONE. 2014;9:e88280 pubmed publisher
	Le T, Nguyen T, Morris G. Monoclonal antibodies for clinical trials of Duchenne muscular dystrophy therapy. Neuromuscul Disord. 2014;24:195-200 pubmed publisher
	Morris G, Man N, Sewry C. Monitoring duchenne muscular dystrophy gene therapy with epitope-specific monoclonal antibodies. Methods Mol Biol. 2011;709:39-61 pubmed publisher
	Nguyen T, Morris G. Use of epitope libraries to identify exon-specific monoclonal antibodies for characterization of altered dystrophins in muscular dystrophy. Am J Hum Genet. 1993;52:1057-66 pubmed
	Mendell J, Kissel J, Amato A, King W, Signore L, Prior T, et al. Myoblast transfer in the treatment of Duchenne's muscular dystrophy. N Engl J Med. 1995;333:832-8 pubmed
	Sedgwick S, Nguyen T, Ellis J, Crowne H, Morris G. Rapid mapping by transposon mutagenesis of epitopes on the muscular dystrophy protein, dystrophin. Nucleic Acids Res. 1991;19:5889-94 pubmed
	Nguyen thi Man -, Cartwright A, Morris G, Love D, Bloomfield J, Davies K. Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin. FEBS Lett. 1990;262:237-40 pubmed

product information

Internal ID :

8262

Name :

MANDYS17(3B12)

Depositor Name :

Morris, G.E.

Depositor Institution :

Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, UK

Date Deposited :

10/23/12

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Dystrophin, exon 26

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Canine,Human,Mouse

Species Tested (legacy) :

human, mouse

Initial Publication Pubmed ID :

1692289

Depositor Notes (Special Instructions) :

Fixation for immunostaining: acetone/methanol.

Collections :

Human,Muscular dystrophy,Skeletal muscle

Search Keywords :

Morris, G.E., Dystrophin, exon 26, Human, MIgG1, Canine/Human/Mouse, DMD, BMD, CMD3B, MRX85, AB_2618167, monoclonal, Human/Muscular dystrophy/Skeletal muscle, Immunofluorescence/Western blot/ELISA

Antigen Molecular Weight :

Predicted: 427 kDa; Apparent: 427 kDa

Gene :

DMD

Alternate Gene Name(s) :

BMD, CMD3B, MRX85

Uniprot ID :

P11532

Antibody Registry ID :

AB_2618167

Immunogen :

Recombinant LacZ/dystrophin (aa 816-1749, rod domain of isoform 4 (Uniprot ID P11532-1)) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

Sp2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

exon 26, aa 1181-1188 (LER-F--K) of isoform 4 (Uniprot ID P11532-1)

Epitope Map PubMed ID :

7684887 24361045

Recommended Applications :

ELISA,Immunofluorescence,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

1692289 24516626 21194020 7651473

Western Blot Pubmed IDs :

1692289 24516626 21194020 1719482

ELISA Pubmed IDs :

1692289

Additional Information :

The collection of anti-DMD mAbs deposited by Morris, G.E., are directed against different DMD exons.

Additional Characterization :

http://www.glennmorris.org.uk/datasheets/MANDYS17(3B12).doc

DSHB Growth Medium :

Iscove's

References (legacy) :

FEBS Lett. 262, 237-240.; Am. J. Hum. Genet. 52, 1057-1066.

more info or order :

Developmental Studies Hybridoma Bank product webpage