Dystrophin, exons 27/28 | Developmental Studies Hybridoma Bank MANDYS16(1B12) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Dystrophin, exons 27/28

catalog :

MANDYS16(1B12)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MANDYS16(1B12)

reactivity :

human, mouse, chicken

application :

western blot, ELISA, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; fig 1	Foltz S, Luan J, Call J, Patel A, Peissig K, Fortunato M, et al. Four-week rapamycin treatment improves muscular dystrophy in a fukutin-deficient mouse model of dystroglycanopathy. Skelet Muscle. 2016;6:20 pubmed publisher
western blot; chicken; 1:5	Wahlin K, Moreira E, Huang H, Yu N, Adler R. Molecular dynamics of photoreceptor synapse formation in the developing chick retina. J Comp Neurol. 2008;506:822-37 pubmed
	Kodippili K, Vince L, Shin J, Yue Y, Morris G, McIntosh M, et al. Characterization of 65 epitope-specific dystrophin monoclonal antibodies in canine and murine models of duchenne muscular dystrophy by immunostaining and western blot. PLoS ONE. 2014;9:e88280 pubmed publisher
	Morris G, Man N, Sewry C. Monitoring duchenne muscular dystrophy gene therapy with epitope-specific monoclonal antibodies. Methods Mol Biol. 2011;709:39-61 pubmed publisher
	Lu Q, Morris G, Wilton S, Ly T, Artem yeva O, Strong P, et al. Massive idiosyncratic exon skipping corrects the nonsense mutation in dystrophic mouse muscle and produces functional revertant fibers by clonal expansion. J Cell Biol. 2000;148:985-96 pubmed
	Hori S, Ohtani S, Nguyen T, Morris G. The N-terminal half of dystrophin is protected from proteolysis in situ. Biochem Biophys Res Commun. 1995;209:1062-7 pubmed
	Nguyen T, Morris G. Use of epitope libraries to identify exon-specific monoclonal antibodies for characterization of altered dystrophins in muscular dystrophy. Am J Hum Genet. 1993;52:1057-66 pubmed
	Sedgwick S, Nguyen T, Ellis J, Crowne H, Morris G. Rapid mapping by transposon mutagenesis of epitopes on the muscular dystrophy protein, dystrophin. Nucleic Acids Res. 1991;19:5889-94 pubmed
	Nguyen thi Man -, Cartwright A, Morris G, Love D, Bloomfield J, Davies K. Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin. FEBS Lett. 1990;262:237-40 pubmed
	Nguyen T, Ginjaar I, van Ommen G, Morris G. Monoclonal antibodies for dystrophin analysis. Epitope mapping and improved binding to SDS-treated muscle sections. Biochem J. 1992;288 ( Pt 2):663-8 pubmed

product information

Internal ID :

3777

Name :

MANDYS16(1B12)

Depositor Name :

Morris, G.E.

Depositor Institution :

Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, UK

Date Deposited :

7/13/06

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Dystrophin, exons 27/28

Antigen Species :

human

Host Species :

mouse

Isotype :

MIgG2b

Isotype for catalog (legacy) :

IgG2b

Positive Tested Species Reactivity :

Human,Mouse

Species Tested (legacy) :

human and mouse

Initial Publication Pubmed ID :

1692289

Search Keywords :

Morris, dystrophin, exons 27/28, DMD, BMD, CMD3B, MRX85, monoclonal, human, AB_2618166, IgG2b, mouse, ELISA,Immunofluorescence,Western Blot

Antigen Molecular Weight :

Predicted: 427 kDa; Apparent: 427 kDa

Gene :

DMD

Alternate Gene Name(s) :

BMD, CMD3B, MRX85

Uniprot ID :

P11532

Antibody Registry ID :

AB_2618166

Immunogen :

Recombinant LacZ/dystrophin (aa 816-1749, rod domain of isoform 4 (Uniprot ID P11532-1)) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

NS0 or Sp2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

exons 27/28, aa 1226-1245

Epitope Map PubMed ID :

7684887

Recommended Applications :

ELISA,Immunofluorescence,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

1692289 21194020 10704448

Western Blot Pubmed IDs :

1692289 24516626 21194020 1719482

ELISA Pubmed IDs :

1692289

Pubmed IDs :

7684887 1281410 7733959

Additional Characterization :

http://www.glennmorris.org.uk/datasheets/MANDYS16(1B12).doc

DSHB Growth Medium :

Iscove's

References (legacy) :

FEBS Lett. 262, 237-240.; Am. J. Hum. Genet. 52, 1057-1066.

more info or order :

Developmental Studies Hybridoma Bank product webpage