Dystrophin, exons 31/32 | Developmental Studies Hybridoma Bank MANDYS15(6B11) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Dystrophin, exons 31/32

catalog :

MANDYS15(6B11)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MANDYS15(6B11)

reactivity :

human, mouse, dog, chicken

application :

western blot, ELISA, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; chicken; 1:5	Wahlin K, Moreira E, Huang H, Yu N, Adler R. Molecular dynamics of photoreceptor synapse formation in the developing chick retina. J Comp Neurol. 2008;506:822-37 pubmed
	Morris G, Man N, Sewry C. Monitoring duchenne muscular dystrophy gene therapy with epitope-specific monoclonal antibodies. Methods Mol Biol. 2011;709:39-61 pubmed publisher
	Nguyen T, Morris G. Use of epitope libraries to identify exon-specific monoclonal antibodies for characterization of altered dystrophins in muscular dystrophy. Am J Hum Genet. 1993;52:1057-66 pubmed
	Mendell J, Kissel J, Amato A, King W, Signore L, Prior T, et al. Myoblast transfer in the treatment of Duchenne's muscular dystrophy. N Engl J Med. 1995;333:832-8 pubmed
	Sedgwick S, Nguyen T, Ellis J, Crowne H, Morris G. Rapid mapping by transposon mutagenesis of epitopes on the muscular dystrophy protein, dystrophin. Nucleic Acids Res. 1991;19:5889-94 pubmed
	Nguyen thi Man -, Cartwright A, Morris G, Love D, Bloomfield J, Davies K. Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin. FEBS Lett. 1990;262:237-40 pubmed

product information

Internal ID :

8215

Name :

MANDYS15(6B11)

Depositor Name :

Morris, G.E.

Depositor Institution :

Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, UK

Date Deposited :

8/29/12

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Dystrophin, exons 31/32

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Canine,Human,Mouse

Species Tested (legacy) :

human, mouse, dog (not chick, not xenopus)

Initial Publication Pubmed ID :

1692289

Depositor Notes (Special Instructions) :

Doesn't work on Chicken or Xenopus. Fixation for immunostaining: acetone/methanol.

Collections :

Human,Muscular dystrophy,Skeletal muscle

Search Keywords :

Morris, G.E., Dystrophin, exons 31/32, Human, MIgG1, Canine/Human/Mouse, DMD, BMD, CMD3B, MRX85, AB_2618165, monoclonal, Human/Muscular dystrophy/Skeletal muscle, Immunofluorescence/Western blot/ELISA

Antigen Molecular Weight :

Predicted: 427 kDa; Apparent: 427 kDa

Gene :

DMD

Alternate Gene Name(s) :

BMD, CMD3B, MRX85

Uniprot ID :

P11532

Antibody Registry ID :

AB_2618165

Immunogen :

Recombinant LacZ/dystrophin (aa 816-1749, rod domain of isoform 4 (Uniprot ID P11532-1)) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

Sp2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

exons 31/32, aa 1431-1505

Epitope Map PubMed ID :

7684887

Recommended Applications :

ELISA,Immunofluorescence,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

1692289 21194020 7651473

Western Blot Pubmed IDs :

1692289 21194020 1719482

ELISA Pubmed IDs :

1692289

Additional Information :

The collection of anti-DMD mAbs deposited by Morris, G.E., are directed against different DMD exons.

Additional Characterization :

http://www.glennmorris.org.uk/datasheets/MANEX1216B(6B11).doc

DSHB Growth Medium :

Iscove's

References (legacy) :

FEBS Lett. 262, 237-240.; Am. J. Hum. Genet. 52, 1057-1066.

more info or order :

Developmental Studies Hybridoma Bank product webpage