product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Dystrophin, exons 31/32
catalog :
MANDYS1(3B7)
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MANDYS1(3B7)
reactivity :
human, mouse, dog, chicken
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation
more info or order :
citations: 20
Published Application/Species/Sample/Dilution | Reference |
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| Wahlin K, Moreira E, Huang H, Yu N, Adler R. Molecular dynamics of photoreceptor synapse formation in the developing chick retina. J Comp Neurol. 2008;506:822-37 pubmed
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Coovert D, Le T, Morris G, Man N, Kralewski M, Sendtner M, et al. Does the survival motor neuron protein (SMN) interact with Bcl-2?. J Med Genet. 2000;37:536-9 pubmed
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Hori S, Ohtani S, Nguyen T, Morris G. The N-terminal half of dystrophin is protected from proteolysis in situ. Biochem Biophys Res Commun. 1995;209:1062-7 pubmed
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Nguyen T, Morris G. Use of epitope libraries to identify exon-specific monoclonal antibodies for characterization of altered dystrophins in muscular dystrophy. Am J Hum Genet. 1993;52:1057-66 pubmed
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Mendell J, Kissel J, Amato A, King W, Signore L, Prior T, et al. Myoblast transfer in the treatment of Duchenne's muscular dystrophy. N Engl J Med. 1995;333:832-8 pubmed
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Sedgwick S, Nguyen T, Ellis J, Crowne H, Morris G. Rapid mapping by transposon mutagenesis of epitopes on the muscular dystrophy protein, dystrophin. Nucleic Acids Res. 1991;19:5889-94 pubmed
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Nguyen thi Man -, Cartwright A, Morris G, Love D, Bloomfield J, Davies K. Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin. FEBS Lett. 1990;262:237-40 pubmed
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Yarom R, Morris G, Froede R, Schaper J. Myocardial dystrophin immunolocalization at sarcolemma and transverse tubules. Experientia. 1992;48:614-6 pubmed
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Lederfein D, Levy Z, Augier N, Mornet D, Morris G, Fuchs O, et al. A 71-kilodalton protein is a major product of the Duchenne muscular dystrophy gene in brain and other nonmuscle tissues. Proc Natl Acad Sci U S A. 1992;89:5346-50 pubmed
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Dickson G, Azad A, Morris G, Simon H, Noursadeghi M, Walsh F. Co-localization and molecular association of dystrophin with laminin at the surface of mouse and human myotubes. J Cell Sci. 1992;103 ( Pt 4):1223-33 pubmed
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Nguyen T, Ginjaar I, van Ommen G, Morris G. Monoclonal antibodies for dystrophin analysis. Epitope mapping and improved binding to SDS-treated muscle sections. Biochem J. 1992;288 ( Pt 2):663-8 pubmed
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product information
Internal ID :
3724
Name :
MANDYS1(3B7)
Depositor Name :
Morris, G.E.
Depositor Institution :
Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, UK
Date Deposited :
10/26/05
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Dystrophin, exons 31/32
Antigen Species :
human
Host Species :
mouse
Isotype :
MIgG2a
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Canine,Human,Mouse,Pig,Rabbit
Species Tested (legacy) :
human, mouse, dog, pig and rabbit
Initial Publication Pubmed ID :
1692289
Depositor Notes (Special Instructions) :
This antibody does not work with Xenopus
Search Keywords :
Morris, dystrophin, exons 31/32, DMD, BMD, CMD3B, MRX85, monoclonal, AB_528206 AB_2314003 AB_2618153, IgG2a, Canine,Human,Mouse,Pig,Rabbit, ELISA,Immunofluorescence,Immunoprecipitation,Western Blot
Antigen Molecular Weight :
Predicted: 427 kDa; Apparent: 427 kDa
Gene :
DMD
Alternate Gene Name(s) :
BMD, CMD3B, MRX85
Uniprot ID :
P11532
Antibody Registry ID :
AB_528206 AB_2314003 AB_2618153
Immunogen :
Recombinant LacZ/dystrophin (aa 816-1749, rod domain of isoform 4 (Uniprot ID P11532-1)) fusion protein
Immunogen Sequence :
Partial protein
Clonality :
Monoclonal
Myeloma Strain :
NS0 or Sp2/0
Epitope Mapped :
Yes
Epitope Location or Sequence :
exons 31/32, aa 1431-1505 (repeats 10 and 11 of the central rod domain)
Epitope Map PubMed ID :
7684887
Recommended Applications :
ELISA,Immunofluorescence,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
27444300
Immunofluorescence Pubmed IDs :
1692289 23893976 7684887 24361045 24516626 21194020 7651473 1612140 1283164
Western Blot Pubmed IDs :
1692289 24516626 21194020 1719482 1283164 1319059
Immunoprecipitation Pubmed IDs :
21194020 1283164 1319059 10970187
ELISA Pubmed IDs :
1692289
Pubmed IDs :
7684887 1281410 7733959
Additional Characterization :
http://www.glennmorris.org.uk/datasheets/MANDYS1(3B7).doc
DSHB Growth Medium :
Iscove's
References (legacy) :
FEBS Lett. 262, 237-240.; Am. J. Hum. Genet. 52, 1057-1066.; J. Anim. Sci. 91, 4116-4122.; J. Anim. Sci 92(8), 3291-3299.
more info or order :
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