dystrophin | Developmental Studies Hybridoma Bank MANDRA1(7A10) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

dystrophin

catalog :

MANDRA1(7A10)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MANDRA1(7A10)

reactivity :

human, mouse, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section, immunohistochemistry knockout validation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry knockout validation; mouse; 1:10; fig 3	Gonzalez J, Crassous P, Schneider J, Beuve A, Fraidenraich D. Neuronal nitric oxide synthase localizes to utrophin expressing intercalated discs and stabilizes their structural integrity. Neuromuscul Disord. 2015;25:964-76 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 9	Sinadinos A, Young C, Al Khalidi R, Teti A, Kalinski P, Mohamad S, et al. P2RX7 purinoceptor: a therapeutic target for ameliorating the symptoms of duchenne muscular dystrophy. PLoS Med. 2015;12:e1001888 pubmed publisher
immunohistochemistry; zebrafish ; 1:10	Ruparelia A, Oorschot V, Vaz R, Ramm G, Bryson Richardson R. Zebrafish models of BAG3 myofibrillar myopathy suggest a toxic gain of function leading to BAG3 insufficiency. Acta Neuropathol. 2014;128:821-33 pubmed publisher
	MassouridÃ¨s E, Polentes J, Mangeot P, Mournetas V, Nectoux J, Deburgrave N, et al. Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells. Skelet Muscle. 2015;5:40 pubmed publisher
	Fero K, Bergeron S, Horstick E, Codore H, Li G, Ono F, et al. Impaired embryonic motility in dusp27 mutants reveals a developmental defect in myofibril structure. Dis Model Mech. 2014;7:289-98 pubmed publisher
	Morris G, Sedgwick S, Ellis J, Pereboev A, Chamberlain J, Nguyen thi Man -. An epitope structure for the C-terminal domain of dystrophin and utrophin. Biochemistry. 1998;37:11117-27 pubmed
	Hori S, Ohtani S, Nguyen T, Morris G. The N-terminal half of dystrophin is protected from proteolysis in situ. Biochem Biophys Res Commun. 1995;209:1062-7 pubmed
	Nguyen T, Ginjaar I, van Ommen G, Morris G. Monoclonal antibodies for dystrophin analysis. Epitope mapping and improved binding to SDS-treated muscle sections. Biochem J. 1992;288 ( Pt 2):663-8 pubmed

product information

Internal ID :

3706

Name :

MANDRA1(7A10)

Depositor Name :

Morris, G.E.

Depositor Institution :

Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, UK

Date Deposited :

10/26/05

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

dystrophin

Antigen Species :

human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Bear,Fish,Human,Mouse,Xenopus

Species Tested (legacy) :

human, mouse, teleost fish, Xenopus and zebrafish

Initial Publication Pubmed ID :

1281410

Depositor Notes (Special Instructions) :

This antibody does not work with torpedo

Search Keywords :

Morris, dystrophin, dmd, monoclonal, IgG2b, Fish,Human,Mouse,Xenopus, Immunofluorescence,Western Blot

Gene :

DMD

Uniprot ID :

P11532

Antibody Registry ID :

AB_2618143

Clonality :

Monoclonal

Epitope Mapped :

Yes

Epitope Location or Sequence :

ex77,aa-PSS by phage-display

Recommended Applications :

Immunofluorescence,Western Blot

Immunohistochemistry Pubmed IDs :

30998788

Immunofluorescence Pubmed IDs :

31361156

Western Blot Pubmed IDs :

26568816

Pubmed IDs :

9693008 7733959

Additional Information :

RRID:AB_2618143

Additional Characterization :

http://www.glennmorris.org.uk/datasheets/MANDRA1(7A10).doc

DSHB Growth Medium :

Iscove's

References (legacy) :

The Lancet 336, 881-882.; Biochem. J. 288, 663-668.; Proc. Natl. Acad. Sci. USA 89, 5346-5350.; Hum. Mol. Genet. 4, 837-842.; Biochem. 37, 11117-11127.; BMC Dev. Biol. 12:9.

more info or order :

Developmental Studies Hybridoma Bank product webpage