product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
dystroglycan, beta-
catalog :
MANDAG2(7D11)
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MANDAG2(7D11)
reactivity :
human, mouse, rat, Xenopus laevis
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - frozen section
citations: 16
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:500; loading ...; fig 9b
Torres A, Rodríguez Escribà M, Marcet Houben M, Santos Vieira H, Camacho N, Catena H, et al. Human tRNAs with inosine 34 are essential to efficiently translate eukarya-specific low-complexity proteins. Nucleic Acids Res. 2021;49:7011-7034 pubmed publisher
  • western blot; human; 1:1500; fig 1b
Ortiz Cordero C, Magli A, Dhoke N, Kuebler T, Selvaraj S, Oliveira N, et al. NAD+ enhances ribitol and ribose rescue of α-dystroglycan functional glycosylation in human FKRP-mutant myotubes. elife. 2021;10: pubmed publisher
  • immunoprecipitation; mouse; loading ...; fig 6a
  • western blot; mouse; loading ...; fig 6a
Yatsenko A, Kucherenko M, Xie Y, Aweida D, Urlaub H, Scheibe R, et al. Profiling of the muscle-specific dystroglycan interactome reveals the role of Hippo signaling in muscular dystrophy and age-dependent muscle atrophy. BMC Med. 2020;18:8 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; fig 6
  • western blot; mouse; 1:100; fig 6
  • western blot; rat; 1:100
Vanhoutte D, Schips T, Kwong J, Davis J, Tjondrokoesoemo A, Brody M, et al. Thrombospondin expression in myofibers stabilizes muscle membranes. elife. 2016;5: pubmed publisher
  • immunohistochemistry; mouse; fig 5
Wang E, Geng A, Maniar A, Mui B, Gong X. Connexin 50 Regulates Surface Ball-and-Socket Structures and Fiber Cell Organization. Invest Ophthalmol Vis Sci. 2016;57:3039-46 pubmed publisher
  • immunohistochemistry - frozen section; human; loading ...; fig 7fs2k
Praissman J, Willer T, Sheikh M, Toi A, Chitayat D, Lin Y, et al. The functional O-mannose glycan on ?-dystroglycan contains a phospho-ribitol primed for matriglycan addition. elife. 2016;5: pubmed publisher
  • immunohistochemistry; Xenopus laevis; 1:100; fig 5
Tandon P, Wilczewski C, Williams C, Conlon F. The Lhx9-integrin pathway is essential for positioning of the proepicardial organ. Development. 2016;143:831-40 pubmed publisher
  • western blot; mouse; 1:250; fig 5
Parvatiyar M, Marshall J, Nguyen R, Jordan M, Richardson V, Roos K, et al. Sarcospan Regulates Cardiac Isoproterenol Response and Prevents Duchenne Muscular Dystrophy-Associated Cardiomyopathy. J Am Heart Assoc. 2015;4: pubmed publisher
  • western blot; mouse; 1:250; fig 4
Marshall J, Oh J, Chou E, Lee J, Holmberg J, Burkin D, et al. Sarcospan integration into laminin-binding adhesion complexes that ameliorate muscular dystrophy requires utrophin and α7 integrin. Hum Mol Genet. 2015;24:2011-22 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500
  • western blot; mouse
Vannoy C, Xu L, Keramaris E, Lu P, Xiao X, Lu Q. Adeno-associated virus-mediated overexpression of LARGE rescues ?-dystroglycan function in dystrophic mice with mutations in the fukutin-related protein. Hum Gene Ther Methods. 2014;25:187-96 pubmed publisher
Trajanovska S, Ban J, Huang J, Gregorevic P, Morsch M, Allen D, et al. Muscle specific kinase protects dystrophic mdx mouse muscles from eccentric contraction-induced loss of force-producing capacity. J Physiol. 2019;597:4831-4850 pubmed publisher
Ma R, Jacobs C, Sharma P, Kocha K, Huang P. Stereotypic generation of axial tenocytes from bipartite sclerotome domains in zebrafish. PLoS Genet. 2018;14:e1007775 pubmed publisher
Zhang Z, Zhang P, Hu H. LARGE expression augments the glycosylation of glycoproteins in addition to ?-dystroglycan conferring laminin binding. PLoS ONE. 2011;6:e19080 pubmed publisher
Morris G. Dystrophin is replaced by utrophin in frog heart; implications for muscular dystrophy. Neuromuscul Disord. 1997;7:493-8 pubmed
James M, Nguyen T, Wise C, Jones G, Morris G. Utrophin-dystroglycan complex in membranes of adherent cultured cells. Cell Motil Cytoskeleton. 1996;33:163-74 pubmed
Helliwell T, Nguyen T, Morris G. Expression of the 43 kDa dystrophin-associated glycoprotein in human neuromuscular disease. Neuromuscul Disord. 1994;4:101-13 pubmed
product information
Internal ID :
3700
Name :
MANDAG2(7D11)
Depositor Name :
Morris, G.E.
Depositor Institution :
Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, UK
Date Deposited :
10/26/05
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
dystroglycan, beta-
Antigen Species :
human
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1, lambda light chain
Positive Tested Species Reactivity :
Human,Xenopus,Zebrafish
Species Tested (legacy) :
mouse, human, Xenopus, zebrafish (probably broad specificity)
Initial Publication Pubmed ID :
8012191
Search Keywords :
Morris, dystroglycan, beta-, mouse
Gene :
DMD
Uniprot ID :
P11532
Antibody Registry ID :
AB_2618140
Clonality :
Monoclonal
Epitope Mapped :
Yes
Epitope Location or Sequence :
aa-SPPPYVP by phage display
Immunofluorescence Pubmed IDs :
30388110 31340406
Western Blot Pubmed IDs :
31340406
Pubmed IDs :
8674136 9447606
Additional Information :
RRID:AB_2618140
Additional Characterization :
http://www.glennmorris.org.uk/datasheets/MANDAG2(7D11).doc
DSHB Growth Medium :
Iscove's
References (legacy) :
Biochim. Biophys. Acta, 1527, 54-60.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US