utrophin | Developmental Studies Hybridoma Bank MANCHO3(8A4) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

utrophin

catalog :

MANCHO3(8A4)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MANCHO3(8A4)

reactivity :

human, mouse, dog

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:50; fig 6	Vanhoutte D, Schips T, Kwong J, Davis J, Tjondrokoesoemo A, Brody M, et al. Thrombospondin expression in myofibers stabilizes muscle membranes. elife. 2016;5: pubmed publisher
immunohistochemistry; mouse; 1:50; fig 5 western blot; mouse; 1:500; fig 5, 6	Tjondrokoesoemo A, Schips T, Kanisicak O, Sargent M, Molkentin J. Genetic overexpression of Serpina3n attenuates muscular dystrophy in mice. Hum Mol Genet. 2016;25:1192-202 pubmed publisher
western blot; mouse; 1:50; fig 5	Parvatiyar M, Marshall J, Nguyen R, Jordan M, Richardson V, Roos K, et al. Sarcospan Regulates Cardiac Isoproterenol Response and Prevents Duchenne Muscular Dystrophy-Associated Cardiomyopathy. J Am Heart Assoc. 2015;4: pubmed publisher
immunocytochemistry; mouse; 1:50; loading ...; fig 7m western blot; mouse; 1:50; loading ...; fig 7a	Van Ry P, Wuebbles R, Key M, Burkin D. Galectin-1 Protein Therapy Prevents Pathology and Improves Muscle Function in the mdx Mouse Model of Duchenne Muscular Dystrophy. Mol Ther. 2015;23:1285-1297 pubmed publisher
western blot; mouse; 1:50; fig 4	Marshall J, Oh J, Chou E, Lee J, Holmberg J, Burkin D, et al. Sarcospan integration into laminin-binding adhesion complexes that ameliorate muscular dystrophy requires utrophin and α7 integrin. Hum Mol Genet. 2015;24:2011-22 pubmed publisher
	Morris G, Sedgwick S, Ellis J, Pereboev A, Chamberlain J, Nguyen thi Man -. An epitope structure for the C-terminal domain of dystrophin and utrophin. Biochemistry. 1998;37:11117-27 pubmed
	Morris G. Dystrophin is replaced by utrophin in frog heart; implications for muscular dystrophy. Neuromuscul Disord. 1997;7:493-8 pubmed
	Cohen M, Jacobson C, Yurchenco P, Morris G, Carbonetto S. Laminin-induced clustering of dystroglycan on embryonic muscle cells: comparison with agrin-induced clustering. J Cell Biol. 1997;136:1047-58 pubmed
	James M, Nguyen T, Wise C, Jones G, Morris G. Utrophin-dystroglycan complex in membranes of adherent cultured cells. Cell Motil Cytoskeleton. 1996;33:163-74 pubmed
	Nguyen T, Ellis J, Love D, Davies K, Gatter K, Dickson G, et al. Localization of the DMDL gene-encoded dystrophin-related protein using a panel of nineteen monoclonal antibodies: presence at neuromuscular junctions, in the sarcolemma of dystrophic skeletal muscle, in vascular and other smooth muscles, and in proli. J Cell Biol. 1991;115:1695-700 pubmed

product information

Internal ID :

3694

Name :

MANCHO3(8A4)

Depositor Name :

Morris, G.E.

Depositor Institution :

Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, UK

Date Deposited :

10/26/05

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

utrophin

Antigen Species :

human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Canine,Human,Mouse,Xenopus

Species Tested (legacy) :

human, mouse, dog and Xenopus

Initial Publication Pubmed ID :

1757469

Depositor Notes (Special Instructions) :

This antibody does not work with fish

Search Keywords :

Morris, utrophin, dmd, monoclonal, IgG2a, Canine,Human,Mouse,Xenopus, Immunofluorescence,Immunohistochemistry,Western Blot

Gene :

DMD

Uniprot ID :

P11532

Antibody Registry ID :

AB_2618137

Clonality :

Monoclonal

Epitope Mapped :

Yes

Epitope Location or Sequence :

3232-3273

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunofluorescence Pubmed IDs :

31340406

Western Blot Pubmed IDs :

31340406

Pubmed IDs :

9693008 8674136 9060469 9447606

Additional Information :

Antigen retrieval may be necessary for FFPE RRID:AB_2618137

Additional Characterization :

http://www.glennmorris.org.uk/datasheets/MANCHO3(8A4).doc

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 115, 1695-1700.; Neuromus. Disorders 2, 177-184.; Cell Motil. Cytoskeleton 33, 163-174.; Biochem. 37, 11117-11127.

more info or order :

Developmental Studies Hybridoma Bank product webpage