Collagen, type IV, alpha 1/ collagen, type IV alpha 2 | Developmental Studies Hybridoma Bank M3F7 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Collagen, type IV, alpha 1/ collagen, type IV alpha 2

catalog :

M3F7

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

M3F7

reactivity :

human, rat, zebrafish

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; zebrafish ; 1:50; loading ...; fig s2c, s3	McGowan L, Kague E, Vorster A, Newham E, Cross S, Hammond C. Wnt16 Elicits a Protective Effect Against Fractures and Supports Bone Repair in Zebrafish. JBMR Plus. 2021;5:e10461 pubmed publisher
western blot; human; loading ...; fig 4a	Sun Y, Yang Y, Keller K. Myosin-X Silencing in the Trabecular Meshwork Suggests a Role for Tunneling Nanotubes in Outflow Regulation. Invest Ophthalmol Vis Sci. 2019;60:843-851 pubmed publisher
immunocytochemistry; human; 1:5	Pautke C, Schieker M, Tischer T, Kolk A, Neth P, Mutschler W, et al. Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts. Anticancer Res. 2004;24:3743-8 pubmed
	Vogelaar C, KÃ¶nig B, Krafft S, Estrada V, Brazda N, Ziegler B, et al. Pharmacological Suppression of CNS Scarring by Deferoxamine Reduces Lesion Volume and Increases Regeneration in an In Vitro Model for Astroglial-Fibrotic Scarring and in Rat Spinal Cord Injury In Vivo. PLoS ONE. 2015;10:e0134371 pubmed publisher
	Saghizadeh M, Winkler M, Kramerov A, Hemmati D, Ghiam C, Dimitrijevich S, et al. A simple alkaline method for decellularizing human amniotic membrane for cell culture. PLoS ONE. 2013;8:e79632 pubmed publisher
	Sillat T, Saat R, Pöllänen R, Hukkanen M, Takagi M, Konttinen Y. Basement membrane collagen type IV expression by human mesenchymal stem cells during adipogenic differentiation. J Cell Mol Med. 2012;16:1485-95 pubmed publisher
	Chuykin I, Lapidus I, Popova E, Vilianovich L, Mosienko V, Alenina N, et al. Characterization of trophoblast and extraembryonic endoderm cell lineages derived from rat preimplantation embryos. PLoS ONE. 2010;5:e9794 pubmed publisher
	Kairuz E, Upton Z, Dawson R, Malda J. Hyperbaric oxygen stimulates epidermal reconstruction in human skin equivalents. Wound Repair Regen. 2007;15:266-74 pubmed
	McMillan J, Akiyama M, Nakamura H, Shimizu H. Colocalization of multiple laminin isoforms predominantly beneath hemidesmosomes in the upper lamina densa of the epidermal basement membrane. J Histochem Cytochem. 2006;54:109-18 pubmed
	Bürgi Saville M, Gerber H, Peter H, Paulsson M, Aeschlimann D, Glaser C, et al. Expression patterns of extracellular matrix components in native and cultured normal human thyroid tissue and in human toxic adenoma tissue. Thyroid. 1997;7:347-56 pubmed
	Ljubimov A, Burgeson R, Butkowski R, Michael A, Sun T, Kenney M. Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms. Lab Invest. 1995;72:461-73 pubmed
	Foellmer H, Madri J, Furthmayr H. Methods in laboratory investigation. Monoclonal antibodies to type IV collagen: probes for the study of structure and function of basement membranes. Lab Invest. 1983;48:639-49 pubmed

product information

Internal ID :

3214

Name :

M3F7

Depositor Name :

Furthmayr, H.

Depositor Institution :

Stanford University

Date Deposited :

8/25/88

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Collagen, type IV, alpha 1/ collagen, type IV alpha 2

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Human,Rat

Species Tested (legacy) :

human, rat

Initial Publication Pubmed ID :

6682465

Depositor Notes (Special Instructions) :

M3F7 recognizes type IV collagen in basement membranes in kidney, lung, placenta, cornea and skin. This antibody does not recognize denatured type IV collagen.

Collections :

Cell adhesion,Cell signaling,Extracellular matrix proteins,Human,Stem cell

Search Keywords :

Heinz Furthmayr, Collagen, type IV, alpha 1/ collagen, type IV alpha 2, Human, MIgG1, kappa light chain, Human/Rat, COL4A1/COL4A2, , AB_528167, Monoclonal, Human/Extracellular Matrix Proteins, Immunohistochemistry/Immunofluorescence/Western blot/ELISA/Immunoprecipitation

Antigen Molecular Weight :

100 kDa/ 50 kDa

Gene :

COL4A1/COL4A2

Uniprot ID :

P02462

Antibody Registry ID :

AB_528167

Immunogen :

Human placenta

Immunogen Sequence :

alpha1 (IV)-alpha2(IV) triple helix

Clonality :

Monoclonal

Myeloma Strain :

SP2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

Triple helical domain about 900 Å from the N-terminus

Epitope Map PubMed ID :

6682465

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

9226202 17352760

Immunofluorescence Pubmed IDs :

6682465 7723285 16174790

Immunoprecipitation Pubmed IDs :

6682465

ELISA Pubmed IDs :

6682465

Additional Information :

RRID:AB_528167

DSHB Growth Medium :

RPMI

References (legacy) :

Lab. Invest. 48, 639-649.; "Protides Biol. Fluids", Peeters, H. vol. 29, pp 773-776.; J. Tiss. Cult. Meth. 14, 31-36.; Lab. Invest. 72, 461-473.; Thyroid 7(3), 347-356.; J. Histochem. Cytochem. 54(1), 109-118.; Wound Rep. Reg. 15, 266-274.

more info or order :

Developmental Studies Hybridoma Bank product webpage