CD18 (integrin beta-2; beta subunit of CD11a, b, c), murine | Developmental Studies Hybridoma Bank M18/2.a.12.7 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

CD18 (integrin beta-2; beta subunit of CD11a, b, c), murine

catalog :

M18/2.a.12.7

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

M18/2.a.12.7

reactivity :

mouse

application :

western blot, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Reference
Núñez Hernández C, Tierrez A, Ortega A, Pucciarelli M, Godoy M, Eisman B, et al. Genome expression analysis of nonproliferating intracellular Salmonella enterica serovar Typhimurium unravels an acid pH-dependent PhoP-PhoQ response essential for dormancy. Infect Immun. 2013;81:154-65 pubmed publisher
Rotshenker S. Microglia and macrophage activation and the regulation of complement-receptor-3 (CR3/MAC-1)-mediated myelin phagocytosis in injury and disease. J Mol Neurosci. 2003;21:65-72 pubmed
Reichert F, Rotshenker S. Complement-receptor-3 and scavenger-receptor-AI/II mediated myelin phagocytosis in microglia and macrophages. Neurobiol Dis. 2003;12:65-72 pubmed
Stokes R, Thorson L, Speert D. Nonopsonic and opsonic association of Mycobacterium tuberculosis with resident alveolar macrophages is inefficient. J Immunol. 1998;160:5514-21 pubmed
Richter Dahlfors A, Buchan A, Finlay B. Murine salmonellosis studied by confocal microscopy: Salmonella typhimurium resides intracellularly inside macrophages and exerts a cytotoxic effect on phagocytes in vivo. J Exp Med. 1997;186:569-80 pubmed
Revilla C, Gonzalez A, Conde C, Lopez Hoyos M, Merino J. Treatment with anti-LFA-1 alpha monoclonal antibody selectively interferes with the maturation of CD4- 8+ thymocytes. Immunology. 1997;90:550-6 pubmed
Driessens M, van Hulten P, Zuurbier A, La Riviere G, Roos E. Inhibition and stimulation of LFA-1 and Mac-1 functions by antibodies against murine CD18. Evidence that the LFA-1 binding sites for ICAM-1, -2, and -3 are distinct. J Leukoc Biol. 1996;60:758-65 pubmed
Torigoe T, Millan J, Chan K, Taichman R, Brian A, Reed J, et al. Protein tyrosine kinase p56-Lck regulates lymphocyte function-associated 1 adhesion molecule expression, granule exocytosis, and cytolytic effector function in a cloned T cell. J Exp Med. 1994;180:1115-27 pubmed
Sanchez Madrid F, Simon P, Thompson S, Springer T. Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med. 1983;158:586-602 pubmed
Pont S, Naquet P, Marchetto S, Regnier Vigouroux A, Blanc D, Pierres A, et al. Identification of 5 topographic domains of the mouse LFA-1 molecule: subunit assignment and functional involvement in lymphoid cell interactions. J Immunol. 1986;136:3750-9 pubmed
Krauss J, Mayo Bond L, Rogers C, Weber K, Todd R, Wilson J. An in vivo animal model of gene therapy for leukocyte adhesion deficiency. J Clin Invest. 1991;88:1412-7 pubmed

product information

Internal ID :

2308

Name :

M18/2.a.12.7

Depositor Name :

Springer, T.A.

Depositor Institution :

Center for Blood Research, Boston

Date Deposited :

4/4/89

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

CD18 (integrin beta-2; beta subunit of CD11a, b, c), murine

Antigen Species :

Mouse

Host Species :

rat

Isotype :

RIgG2a, kappa light chain

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Mouse

Species Tested (legacy) :

does not cross react with human antigen

Initial Publication Pubmed ID :

6193226

Depositor Notes (Special Instructions) :

Does not cross react with human antigen. Binds to all leukocytes. Works for western blotting under non-reducing conditions.

Collections :

CD antigens,Cell adhesion,Cell markers,Cell signaling

Search Keywords :

Springer, T.A., CD18 (integrin beta-2; beta subunit of CD11a, b, c), murine, Mouse, RIgG2a, kappa light chain, Mouse, Itgb2, CD18, MFI7, LAD, LCAMB, LFA-1, MAC-1, AB_2128355, monoclonal, Cell signaling/Cluster Determinant antigens/Cell adhesion/Cell markers, Immunofluorescence/Western Blot/Immunoprecipitation/Function Blocking/FACS

Antigen Molecular Weight :

Predicted: 85 kDa; Apparent: 93 kDa

Gene :

Itgb2

Alternate Gene Name(s) :

CD18, MFI7, LAD, LCAMB, LFA-1, MAC-1

Uniprot ID :

P11835

Antibody Registry ID :

AB_2128355

Synonyms (Alternate Clone Names) :

M18/2

Immunogen :

Membrane glycoproteins of cytotoxic T-lymphocytes

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag8.6.5.3

Epitope Mapped :

Recommended Applications :

FACS,Function Blocking,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes (non-reducing conditions)

Immunofluorescence Pubmed IDs :

9254655 23090959

Western Blot Pubmed IDs :

6193226 2422261

Immunoprecipitation Pubmed IDs :

6193226 8975879 2422261

Function Blocking Pubmed IDs :

6193226 14500997 8975879 12609490 7914906

FACS Pubmed IDs :

9605155 1680882 7914906 9176108

Additional Information :

The effect of M18/2.a.12.7 on function is oftentimes more enhancing than inhibiting. CD18 is a general marker of leukocytes.

DSHB Growth Medium :

RPMI

References (legacy) :

J. Exp. Med. 158, 586-602.; J. Immunol. 160, 5514-5521.

more info or order :

Developmental Studies Hybridoma Bank product webpage