product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
CD11a (Integrin alpha-L, LFA-1 alpha subunit), murine
catalog :
M17/4.4.11.9
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
M17/4.4.11.9
reactivity :
mouse
application :
immunohistochemistry, immunoprecipitation, FACS
more info or order :
Developmental Studies Hybridoma Bank product webpage
citations: 11
Reference
Wan M, Schramm R, Klintman D, Welzenbach K, Weitz Schmidt G, Thorlacius H. A statin-based inhibitor of lymphocyte function antigen-1 protects against ischemia/reperfusion-induced leukocyte adhesion in the colon. Br J Pharmacol. 2003;140:395-401 pubmed
Stokes R, Thorson L, Speert D. Nonopsonic and opsonic association of Mycobacterium tuberculosis with resident alveolar macrophages is inefficient. J Immunol. 1998;160:5514-21 pubmed
Driessens M, van Hulten P, Zuurbier A, La Riviere G, Roos E. Inhibition and stimulation of LFA-1 and Mac-1 functions by antibodies against murine CD18. Evidence that the LFA-1 binding sites for ICAM-1, -2, and -3 are distinct. J Leukoc Biol. 1996;60:758-65 pubmed
Torigoe T, Millan J, Chan K, Taichman R, Brian A, Reed J, et al. Protein tyrosine kinase p56-Lck regulates lymphocyte function-associated 1 adhesion molecule expression, granule exocytosis, and cytolytic effector function in a cloned T cell. J Exp Med. 1994;180:1115-27 pubmed
van Kooyk Y, de Vries van der Zwan A, de Waal L, Figdor C. Efficiency of antibodies directed against adhesion molecules to prolong skin graft survival in mice. Transplant Proc. 1994;26:401-3 pubmed
Huang C, Springer T. A binding interface on the I domain of lymphocyte function-associated antigen-1 (LFA-1) required for specific interaction with intercellular adhesion molecule 1 (ICAM-1). J Biol Chem. 1995;270:19008-16 pubmed
Beller D, Springer T, Schreiber R. Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor. J Exp Med. 1982;156:1000-9 pubmed
Sanchez Madrid F, Davignon D, Martz E, Springer T. Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship. Cell Immunol. 1982;73:1-11 pubmed
Kürzinger K, Springer T. Purification and structural characterization of LFA-1, a lymphocyte function-associated antigen, and Mac-1, a related macrophage differentiation antigen associated with the type three complement receptor. J Biol Chem. 1982;257:12412-8 pubmed
Sanchez Madrid F, Simon P, Thompson S, Springer T. Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med. 1983;158:586-602 pubmed
Krauss J, Mayo Bond L, Rogers C, Weber K, Todd R, Wilson J. An in vivo animal model of gene therapy for leukocyte adhesion deficiency. J Clin Invest. 1991;88:1412-7 pubmed
product information
Internal ID :
2302
Name :
M17/4.4.11.9
Depositor Name :
Springer, T.A.
Depositor Institution :
Center for Blood Research, Boston
Date Deposited :
4/4/89
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
CD11a (Integrin alpha-L, LFA-1 alpha subunit), murine
Antigen Species :
Mouse
Host Species :
rat
Isotype :
RIgG2a, kappa light chain
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Mouse
Species Tested (legacy) :
does not cross react with human antigen
Initial Publication Pubmed ID :
6983917
Depositor Notes (Special Instructions) :
Does not cross react with human antigen. Binds to all leukocytes except resident peritoneal macrophages. Doesn't appear to work for western blotting.
Collections :
CD antigens,Cell adhesion,Cell markers,Cell signaling
Search Keywords :
Springer, T.A., CD11a (Integrin alpha-L, LFA-1 alpha subunit), murine, Mouse, RIgG2a, kappa light chain, Mouse, Itgal, LFA-1, Ly-15, AB_2280611, monoclonal, Cell signaling/Cluster Determinant antigens/Cell adhesion/Cell markers, Immunohistochemistry/Immunoprecipitation/Function Blocking/FACS
Antigen Molecular Weight :
Predicted: 128 kDa; Apparent: 180 kDa
Gene :
Itgal
Alternate Gene Name(s) :
LFA-1, Ly-15
Uniprot ID :
P24063
Antibody Registry ID :
AB_2280611
Synonyms (Alternate Clone Names) :
M17/4
Immunogen :
Anti-P815 cytotoxic T-lymphocytes from C57BL/6J mice.
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
NS1 or P3X63Ag8.6.5.3
Epitope Mapped :
Yes
Epitope Location or Sequence :
I domain (aa 248-303)
Epitope Map PubMed ID :
7642561
Recommended Applications :
FACS,Function Blocking,Immunohistochemistry,Immunoprecipitation
Immunoblotting (legacy) :
No
Immunoprecipitation Pubmed IDs :
6983917 6193226 8975879 6749860
Function Blocking Pubmed IDs :
6983917 6193226 7909626 7153706 7914906 12970101
FACS Pubmed IDs :
6983917 9605155 6749860 1680882 7914906 12970101
DSHB Growth Medium :
RPMI
References (legacy) :
J. Exp. Med. 158, 586-602.; J. Immunol. 160, 5514-5521.
more info or order :
Developmental Studies Hybridoma Bank product webpage
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US