CD11b (Integrin alpha-M, Mac-1 alpha chain), murine | Developmental Studies Hybridoma Bank M1/70.15.11.5.2 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

CD11b (Integrin alpha-M, Mac-1 alpha chain), murine

catalog :

M1/70.15.11.5.2

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

M1/70.15.11.5.2

reactivity :

human, mouse

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 29

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; loading ...; fig s2d	Huang H, Liu Y, Wang L, Li W. Age-related macular degeneration phenotypes are associated with increased tumor necrosis-alpha and subretinal immune cells in aged Cxcr5 knockout mice. PLoS ONE. 2017;12:e0173716 pubmed publisher
immunocytochemistry; mouse; loading ...; fig 2k	Zhu J, Cifuentes H, Reynolds J, Lamba D. Immunosuppression via Loss of IL2rÎ³ Enhances Long-Term Functional Integration of hESC-Derived Photoreceptors in the Mouse Retina. Cell Stem Cell. 2017;20:374-384.e5 pubmed publisher
immunohistochemistry; mouse; 1:10; fig 3	Gu X, Liu X, Chen Y, Zhao Y, Xu M, Han X, et al. Involvement of NADPH oxidases in alkali burn-induced corneal injury. Int J Mol Med. 2016;38:75-82 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:50; fig s7	Dooves S, Bugiani M, Postma N, Polder E, Land N, Horan S, et al. Astrocytes are central in the pathomechanisms of vanishing white matter. J Clin Invest. 2016;126:1512-24 pubmed publisher
immunocytochemistry; mouse; fig 1	Paone C, Rodrigues N, Ittner E, Santos C, Buntru A, Hauck C. The Tyrosine Kinase Pyk2 Contributes to Complement-Mediated Phagocytosis in Murine Macrophages. J Innate Immun. 2016;8:437-51 pubmed publisher
immunohistochemistry; mouse; 1:5	Astafurov K, Elhawy E, Ren L, Dong C, Igboin C, Hyman L, et al. Oral microbiome link to neurodegeneration in glaucoma. PLoS ONE. 2014;9:e104416 pubmed publisher
immunohistochemistry - frozen section; mouse	Labarge S, McDonald M, Smith Powell L, Auwerx J, Huss J. Estrogen-related receptor-? (ERR?) deficiency in skeletal muscle impairs regeneration in response to injury. FASEB J. 2014;28:1082-97 pubmed publisher
	Kulkarni U, Karsten C, Kohler T, Hammerschmidt S, Bommert K, Tiburzy B, et al. IL-10 mediates plasmacytosis-associated immunodeficiency by inhibiting complement-mediated neutrophil migration. J Allergy Clin Immunol. 2016;137:1487-1497.e6 pubmed publisher
	Winsauer C, Prepens S, Schlienz D, Nedospasov S, Kruglov A. Novel mouse model to study T cell-dependent IgA induction in vivo. J Immunol Methods. 2015;421:54-60 pubmed publisher
	Zehentmeier S, Roth K, Cseresnyes Z, Sercan O, Horn K, Niesner R, et al. Static and dynamic components synergize to form a stable survival niche for bone marrow plasma cells. Eur J Immunol. 2014;44:2306-17 pubmed publisher
	Dai S, Rajaram M, Curry H, Leander R, Schlesinger L. Fine tuning inflammation at the front door: macrophage complement receptor 3-mediates phagocytosis and immune suppression for Francisella tularensis. PLoS Pathog. 2013;9:e1003114 pubmed publisher
	Shibasaki K, Suzuki M, Mizuno A, Tominaga M. Effects of body temperature on neural activity in the hippocampus: regulation of resting membrane potentials by transient receptor potential vanilloid 4. J Neurosci. 2007;27:1566-75 pubmed
	Hagihara H, Hara M, Tsunekawa K, Nakagawa Y, Sawada M, Nakano K. Tonic-clonic seizures induce division of neuronal progenitor cells with concomitant changes in expression of neurotrophic factors in the brain of pilocarpine-treated mice. Brain Res Mol Brain Res. 2005;139:258-66 pubmed
	Rotshenker S. Microglia and macrophage activation and the regulation of complement-receptor-3 (CR3/MAC-1)-mediated myelin phagocytosis in injury and disease. J Mol Neurosci. 2003;21:65-72 pubmed
	Brickson S, Ji L, Schell K, Olabisi R, St Pierre Schneider B, Best T. M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury. J Appl Physiol (1985). 2003;95:969-76 pubmed
	Reichert F, Rotshenker S. Complement-receptor-3 and scavenger-receptor-AI/II mediated myelin phagocytosis in microglia and macrophages. Neurobiol Dis. 2003;12:65-72 pubmed
	Reichert F, Slobodov U, Makranz C, Rotshenker S. Modulation (inhibition and augmentation) of complement receptor-3-mediated myelin phagocytosis. Neurobiol Dis. 2001;8:504-12 pubmed
	Welt F, Edelman E, Simon D, Rogers C. Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries. Arterioscler Thromb Vasc Biol. 2000;20:2553-8 pubmed
	Driessens M, van Hulten P, Zuurbier A, La Riviere G, Roos E. Inhibition and stimulation of LFA-1 and Mac-1 functions by antibodies against murine CD18. Evidence that the LFA-1 binding sites for ICAM-1, -2, and -3 are distinct. J Leukoc Biol. 1996;60:758-65 pubmed
	Guy R, Belosevic M. Comparison of receptors required for entry of Leishmania major amastigotes into macrophages. Infect Immun. 1993;61:1553-8 pubmed
	Fan S, Edgington T. Integrin regulation of leukocyte inflammatory functions. CD11b/CD18 enhancement of the tumor necrosis factor-alpha responses of monocytes. J Immunol. 1993;150:2972-80 pubmed
	Ault K, Springer T. Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol. 1981;126:359-64 pubmed
	Beller D, Springer T, Schreiber R. Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor. J Exp Med. 1982;156:1000-9 pubmed
	Holmberg L, Springer T, Ault K. Natural killer activity in the peritoneal exudates of mice infected with Listeria monocytogenes: characterization of the natural killer cells by using a monoclonal rat anti-murine macrophage antibody (M1/70). J Immunol. 1981;127:1792-9 pubmed
	Kürzinger K, Springer T. Purification and structural characterization of LFA-1, a lymphocyte function-associated antigen, and Mac-1, a related macrophage differentiation antigen associated with the type three complement receptor. J Biol Chem. 1982;257:12412-8 pubmed
	Sanchez Madrid F, Simon P, Thompson S, Springer T. Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med. 1983;158:586-602 pubmed
	Anderson D, Miller L, Schmalstieg F, Rothlein R, Springer T. Contributions of the Mac-1 glycoprotein family to adherence-dependent granulocyte functions: structure-function assessments employing subunit-specific monoclonal antibodies. J Immunol. 1986;137:15-27 pubmed
	Krauss J, Mayo Bond L, Rogers C, Weber K, Todd R, Wilson J. An in vivo animal model of gene therapy for leukocyte adhesion deficiency. J Clin Invest. 1991;88:1412-7 pubmed
	Sawada M, Wilkinson J, MacAdoo D, Coggeshall R. The identification of two inhibitory cells in each segmental ganglion of the leech and studies on the ionic mechanism of the inhibitory junctional potentials produced by these cells. J Neurobiol. 1976;7:435-45 pubmed

product information

Internal ID :

2296

Name :

M1/70.15.11.5.2

Depositor Name :

Springer, T.A.

Depositor Institution :

Center for Blood Research, Boston

Date Deposited :

4/4/89

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

CD11b (Integrin alpha-M, Mac-1 alpha chain), murine

Antigen Species :

Mouse

Host Species :

rat

Isotype :

RIgG2b

Isotype for catalog (legacy) :

IgG2b

Positive Tested Species Reactivity :

Human,Mouse

Species Tested (legacy) :

cross reacts with human antigen

Initial Publication Pubmed ID :

81133

Depositor Notes (Special Instructions) :

Blocks iC3B rosetting. Binds to granulocytes, monocytes, macrophages, hairy cell leukemia cells, CD5+ human B cells. Doesn't appear to work for western blotting.

Collections :

CD antigens,Cell adhesion,Cell markers,Cell signaling,Extracellular matrix proteins,Human,Stem cell

Search Keywords :

Springer, T.A., CD11b (Integrin alpha-M, Mac-1 alpha chain), murine, Mouse, RIgG2b, Human/Mouse, Itgam, MAC-1, MO1A, CR3, AB_2234066, monoclonal, Human/Extracellular matrix proteins/Cluster Determinant antigens/Cell signaling/Cell adhesion/Cell markers/Stem cell, Immunohistochemistry/Immunofluorescence/Immunoprecipitation/Function Blocking/FACS

Antigen Molecular Weight :

Predicted: 128 kDa; Apparent: 170 kDa

Gene :

Itgam

Alternate Gene Name(s) :

MAC-1, MO1A, CR3

Uniprot ID :

P05555

Antibody Registry ID :

AB_2234066

Synonyms (Alternate Clone Names) :

M1/70

Immunogen :

Spleen cells from B10 mice.

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Recommended Applications :

FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation

Immunoblotting (legacy) :

Immunohistochemistry Pubmed IDs :

185332

Immunofluorescence Pubmed IDs :

16023256 23359218

Immunoprecipitation Pubmed IDs :

81133 89034 6193226 185332 8975879 6749860

Function Blocking Pubmed IDs :

12730143 6193226 12609490 8095957 11442357 8454363 14500997 11116052 3519773 7153706

FACS Pubmed IDs :

89034 6795262 12730143 7451976 25790905 26653800 24777940 185332 6749860 1680882

Pubmed IDs :

17301165

Additional Information :

Mac-1 is a macrophage differenciation antigen and a marker for microglia.

DSHB Growth Medium :

RPMI

References (legacy) :

Eur. J. Immunol. 8, 539-551.; Eur. J. Immunol. 9, 301-306.; J. Neurosci. 27(7), 1566-1575.

more info or order :

Developmental Studies Hybridoma Bank product webpage