collagen(pro-) type I | Developmental Studies Hybridoma Bank M-38 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

collagen(pro-) type I

catalog :

M-38

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

M-38

reactivity :

human, cow

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human	Hjorten R, Hansen U, Underwood R, Telfer H, Fernandes R, Krakow D, et al. Type XXVII collagen at the transition of cartilage to bone during skeletogenesis. Bone. 2007;41:535-42 pubmed
immunocytochemistry; human; 1:5	Pautke C, Schieker M, Tischer T, Kolk A, Neth P, Mutschler W, et al. Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts. Anticancer Res. 2004;24:3743-8 pubmed
	Vazquez M, Evans B, Riccardi D, Evans S, Ralphs J, Dillingham C, et al. A new method to investigate how mechanical loading of osteocytes controls osteoblasts. Front Endocrinol (Lausanne). 2014;5:208 pubmed publisher
	Schminke B, Vom Orde F, Gruber R, Schliephake H, BÃ¼rgers R, Miosge N. The pathology of bone tissue during peri-implantitis. J Dent Res. 2015;94:354-61 pubmed publisher
	Mhanna R, Kashyap A, Palazzolo G, Vallmajo Martin Q, Becher J, Moller S, et al. Chondrocyte culture in three dimensional alginate sulfate hydrogels promotes proliferation while maintaining expression of chondrogenic markers. Tissue Eng Part A. 2014;20:1454-64 pubmed publisher
	Humphrey E, Morris G, Fuller H. Valproate reduces collagen and osteonectin in cultured bone cells. Epilepsy Res. 2013;106:446-50 pubmed publisher
	Cox N, Pilling D, Gomer R. NaCl potentiates human fibrocyte differentiation. PLoS ONE. 2012;7:e45674 pubmed publisher
	Fuller H, Man N, Lam L, Shamanin V, Androphy E, Morris G. Valproate and bone loss: iTRAQ proteomics show that valproate reduces collagens and osteonectin in SMA cells. J Proteome Res. 2010;9:4228-33 pubmed publisher
	Alfieri C, Cheek J, Chakraborty S, Yutzey K. Wnt signaling in heart valve development and osteogenic gene induction. Dev Biol. 2010;338:127-35 pubmed publisher
	Koelling S, Kruegel J, Irmer M, Path J, Sadowski B, Miro X, et al. Migratory chondrogenic progenitor cells from repair tissue during the later stages of human osteoarthritis. Cell Stem Cell. 2009;4:324-35 pubmed publisher
	Gealy C, Hayes A, Buckwell R, Young R, Caterson B, Quantock A, et al. Actin and type I collagen propeptide distribution in the developing chick cornea. Invest Ophthalmol Vis Sci. 2009;50:1653-8 pubmed publisher
	ten Berge D, Brugmann S, Helms J, Nusse R. Wnt and FGF signals interact to coordinate growth with cell fate specification during limb development. Development. 2008;135:3247-57 pubmed publisher
	Brem H, Stojadinovic O, Diegelmann R, Entero H, Lee B, Pastar I, et al. Molecular markers in patients with chronic wounds to guide surgical debridement. Mol Med. 2007;13:30-9 pubmed
	Lucitti J, Visconti R, Novak J, Keller B. Increased arterial load alters aortic structural and functional properties during embryogenesis. Am J Physiol Heart Circ Physiol. 2006;291:H1919-26 pubmed
	Stojadinovic O, Brem H, Vouthounis C, Lee B, Fallon J, Stallcup M, et al. Molecular pathogenesis of chronic wounds: the role of beta-catenin and c-myc in the inhibition of epithelialization and wound healing. Am J Pathol. 2005;167:59-69 pubmed
	Emans P, Surtel D, Frings E, Bulstra S, Kuijer R. In vivo generation of cartilage from periosteum. Tissue Eng. 2005;11:369-77 pubmed
	van de Breevaart Bravenboer J, In der Maur C, Bos P, Feenstra L, Verhaar J, Weinans H, et al. Improved cartilage integration and interfacial strength after enzymatic treatment in a cartilage transplantation model. Arthritis Res Ther. 2004;6:R469-76 pubmed
	Schultheiss D, Lorenz R, Meister R, Westphal M, Gabouev A, Mertsching H, et al. Functional tissue engineering of autologous tunica albuginea: a possible graft for Peyronie's disease surgery. Eur Urol. 2004;45:781-6 pubmed
	Reeves R, Edberg D, Li Y. Architectural transcription factor HMGI(Y) promotes tumor progression and mesenchymal transition of human epithelial cells. Mol Cell Biol. 2001;21:575-94 pubmed
	Roser K, Johansson C, Donath K, Albrektsson T. A new approach to demonstrate cellular activity in bone formation adjacent to implants. J Biomed Mater Res. 2000;51:280-91 pubmed
	Aulthouse A, Hitt D. The teratogenic effects of valproic acid in human chondrogenesis in vitro. Teratology. 1994;49:208-17 pubmed
	McDonald J, Broekelmann T, Matheke M, Crouch E, Koo M, Kuhn C. A monoclonal antibody to the carboxyterminal domain of procollagen type I visualizes collagen-synthesizing fibroblasts. Detection of an altered fibroblast phenotype in lungs of patients with pulmonary fibrosis. J Clin Invest. 1986;78:1237-44 pubmed
	Melkko J, Niemi S, Risteli L, Risteli J. Radioimmunoassay of the carboxyterminal propeptide of human type I procollagen. Clin Chem. 1990;36:1328-32 pubmed
	Mould A, Hulmes D, Holmes D, Cummings C, Sear C, Chapman J. D-periodic assemblies of type I procollagen. J Mol Biol. 1990;211:581-94 pubmed

product information

Internal ID :

3434

Name :

M-38

Depositor Name :

McDonald, J.A.

Depositor Institution :

Mayo Clinic Scottsdale

Date Deposited :

2/11/87

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

collagen(pro-) type I

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Bovine,Chicken,Guinea Pig,Human

Species Tested (legacy) :

reacts with human, chicken, guinea pig, calf procollagen type I. No reaction with mouse, rat, hamster.

Initial Publication Pubmed ID :

3771795

Depositor Notes (Special Instructions) :

The epitope recognized by this antibody is destroyed by formalin. Frozen or acid alcohol fixed tissue is recommended. This antibody does not recognize mouse, rat or hamster procollagen type I.

Collections :

Cell adhesion,Extracellular matrix proteins,Human

Search Keywords :

McDonald, John A. , collagen(pro-) type I , Human, MIgG1, Human/Chicken/Guinea pig/Bovine, COL1A1, OI4, AB_2245128, Monoclonal, Human/Extracellular Matrix Proteins, Western blot/Immunohistochemistry/Immunofluorescence/FACS/FFPE/ELISA/Immunoprecipitation

Antigen Molecular Weight :

140-150 kDa

Gene :

COL1A1

Alternate Gene Name(s) :

OI4

Uniprot ID :

P02452

Antibody Registry ID :

AB_2245128

Immunogen :

Ascorbate-supplemented human IMR-90 cells (lung fibroblast)

Immunogen Sequence :

Carboxyterminal propeptide type I collagen

Clonality :

Monoclonal

Myeloma Strain :

SP-2/0-Ag 14

Epitope Mapped :

Yes

Epitope Map PubMed ID :

2308167

Recommended Applications :

ELISA,FACS,FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Immunohistochemistry Pubmed IDs :

3771795 23029177 18776145 17693149 17515955 16648183 15869417 15380046 15149752 11134344 10825228 8059428

Immunofluorescence Pubmed IDs :

25538684 24320935 23906561 20568814 19961844 19341622 19074811 18776145

Western Blot Pubmed IDs :

3771795 25406169 2372946

Immunoprecipitation Pubmed IDs :

2308167

ELISA Pubmed IDs :

2372946

FFPE Pubmed IDs :

17515955 15972952 11134344

FACS Pubmed IDs :

25406169

Additional Information :

RRID:AB_2245128

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Clin. Invest. 78, 1237-1244.; J. Mol. Biol. 211, 581-594.; Clinical Chem. 36, 1328-1332.; J. Tiss. Cult. Meth. 14, 31-36.; Teratology 49, 208-217.; Clin. Imp. Dent. Rel. Res. 1(1), 33-40.; J. Biomed. Mater. Res. 51, 280-291.; Tissue Eng. 11(3-4), 369-377.; J. Proteome Res. 9, 4228-4233.; Epilepsy Res. 106(3), 446-450.

more info or order :

Developmental Studies Hybridoma Bank product webpage