LMP-1 | Developmental Studies Hybridoma Bank LMP1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

LMP-1

catalog :

LMP1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

LMP1

reactivity :

Caenorhabditis elegans

application :

western blot, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 5

Reference
Huynh J, Dang H, Munoz Tucker I, O Ketch M, Liu I, Perno S, et al. ESCRT-Dependent Cell Death in a Caenorhabditis elegans Model of the Lysosomal Storage Disorder Mucolipidosis Type IV. Genetics. 2016;202:619-38 pubmed publisher
Djeddi A, Al Rawi S, Deuve J, Perrois C, Liu Y, Russeau M, et al. Sperm-inherited organelle clearance in C. elegans relies on LC3-dependent autophagosome targeting to the pericentrosomal area. Development. 2015;142:1705-16 pubmed publisher
Tanji T, Nishikori K, Shiraishi H, Maeda M, Ohashi Kobayashi A. Co-operative function and mutual stabilization of the half ATP-binding cassette transporters HAF-4 and HAF-9 in Caenorhabditis elegans. Biochem J. 2013;452:467-75 pubmed publisher
Cheng L, Li B, Wang Z. Level-Set Minimization of Potential Controlled Hadwiger Valuations for Molecular Solvation. J Comput Phys. 2010;229:8497-8510 pubmed
Hadwiger G, Dour S, Arur S, Fox P, Nonet M. A monoclonal antibody toolkit for C. elegans. PLoS ONE. 2010;5:e10161 pubmed publisher

product information

Internal ID :

6498

Name :

LMP1

Depositor Name :

Nonet, M.L. / Hadwiger, G. / Dour, S.

Depositor Institution :

Washington University Medical School

Date Deposited :

2/16/10

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

LMP-1

Antigen Species :

C. elegans

Host Species :

mouse

Isotype :

MIgG2b

Isotype for catalog (legacy) :

IgG2b

Positive Tested Species Reactivity :

C. elegans

Species Tested (legacy) :

C. elegans

Initial Publication Pubmed ID :

20405020

Depositor Notes (Special Instructions) :

Works on C. elegans whole mounts. Stains lysosomes. Specific by western blot: the 37 kDa band observed in wild type is absent from the lmp-1 mutant (nr2045). On whole mount immunostaining, most of the wild type staining is lost in the lmp-1 mutant (nr2045) except that the immunostaining of the nervous system remained in the mutant and became more pronounced, suggesting some cross reaction with another LAMP protein (see initial publication).

Collections :

C. elegans

Search Keywords :

Nonet, M.L. / Hadwiger, G. / Dour, S., LMP-1, C. elegans, MIgG2b, C. elegans, LMP-1, , AB_2161795, monoclonal, C. elegans, Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: 24 kDa; Apparent: 37 kDa

Gene :

LMP-1

Uniprot ID :

Q11117

Antibody Registry ID :

AB_2161795

Synonyms (Alternate Clone Names) :

2E2

Immunogen :

Recombinant His6-tagged LMP-1 (N-terminal aa 1-143 of mature LMP-1) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

N-terminal aa 1-143 of mature LMP-1

Epitope Map PubMed ID :

20405020

Recommended Applications :

Immunofluorescence,Western Blot

Immunoblotting (legacy) :

good

Immunofluorescence Pubmed IDs :

20405020 25922527

Western Blot Pubmed IDs :

20405020 23458156 26596346

Additional Information :

The LAMP family protein lmp-1 is a homolog of the lysosome associated membrane protein family (LAMPs) and is highly expressed and localized to lysosomal-like granules in the C. elegans intestine which can be detected in wild type animals.

DSHB Growth Medium :

Iscove's

References (legacy) :

Hadwiger, G., Dour, S., Arur, S., Fox, P., and Nonet, M.L. (2010) A monoclonal antibody toolkit for C. elegans. PLoS One. 5(4):e10161-10115.

more info or order :

Developmental Studies Hybridoma Bank product webpage