CD11b (Integrin alpha-M, Mac-1 alpha chain), human | Developmental Studies Hybridoma Bank LM2/1.6.11 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

CD11b (Integrin alpha-M, Mac-1 alpha chain), human

catalog :

LM2/1.6.11

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

LM2/1.6.11

reactivity :

human

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 13

Reference
Pluskota E, Soloviev D, Bdeir K, Cines D, Plow E. Integrin alphaMbeta2 orchestrates and accelerates plasminogen activation and fibrinolysis by neutrophils. J Biol Chem. 2004;279:18063-72 pubmed
Edwards J, Apicella M. The role of lipooligosaccharide in Neisseria gonorrhoeae pathogenesis of cervical epithelia: lipid A serves as a C3 acceptor molecule. Cell Microbiol. 2002;4:585-98 pubmed
Lee Y, Nagai A, Kim S. Cytokines, chemokines, and cytokine receptors in human microglia. J Neurosci Res. 2002;69:94-103 pubmed
Saltzman W, Livingston T, Parkhurst M. Antibodies to CD18 influence neutrophil migration through extracellular matrix. J Leukoc Biol. 1999;65:356-63 pubmed
Boackle S, Morris M, Holers V, Karp D. Complement opsonization is required for presentation of immune complexes by resting peripheral blood B cells. J Immunol. 1998;161:6537-43 pubmed
Cywes C, Godenir N, Hoppe H, Scholle R, Steyn L, Kirsch R, et al. Nonopsonic binding of Mycobacterium tuberculosis to human complement receptor type 3 expressed in Chinese hamster ovary cells. Infect Immun. 1996;64:5373-83 pubmed
Forsyth C, Mathews H. A quantitative radiometric assay to measure mammalian cell binding to hyphae of Candida albicans. J Immunol Methods. 1993;165:113-9 pubmed
Diamond M, Garcia Aguilar J, Bickford J, Corbi A, Springer T. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands. J Cell Biol. 1993;120:1031-43 pubmed
McGuire S, Bajt M. Distinct ligand binding sites in the I domain of integrin alpha M beta 2 that differentially affect a divalent cation-dependent conformation. J Biol Chem. 1995;270:25866-71 pubmed
Anderson D, Miller L, Schmalstieg F, Rothlein R, Springer T. Contributions of the Mac-1 glycoprotein family to adherence-dependent granulocyte functions: structure-function assessments employing subunit-specific monoclonal antibodies. J Immunol. 1986;137:15-27 pubmed
Miller L, Schwarting R, Springer T. Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation. J Immunol. 1986;137:2891-900 pubmed
Buyon J, Slade S, Reibman J, Abramson S, Philips M, Weissmann G, et al. Constitutive and induced phosphorylation of the alpha- and beta-chains of the CD11/CD18 leukocyte integrin family. Relationship to adhesion-dependent functions. J Immunol. 1990;144:191-7 pubmed
Beekhuizen H, Corsèl van Tilburg A, Blokland I, Van Furth R. Characterization of the adherence of human monocytes to cytokine-stimulated human macrovascular endothelial cells. Immunology. 1991;74:661-9 pubmed

product information

Internal ID :

2290

Name :

LM2/1.6.11

Depositor Name :

Springer, T.A.

Depositor Institution :

Center for Blood Research, Boston

Date Deposited :

4/4/89

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

CD11b (Integrin alpha-M, Mac-1 alpha chain), human

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human

Species Tested (legacy) :

does not cross react with mouse

Initial Publication Pubmed ID :

2428876

Depositor Notes (Special Instructions) :

Does not cross react with mouse. Does not block iC3B rosetting. Binds to granulocytes, monocytes, macrophages, CD5+ B cells, hairy cell leukemia cells. Doesn't work well for western blotting.

Collections :

CD antigens,Cell adhesion,Cell markers,Cell signaling,Extracellular matrix proteins,Human,Stem cell

Search Keywords :

Springer, T.A., CD11b (Integrin alpha-M, Mac-1 alpha chain), human, Human, MIgG1, Human, ITGAM, MAC-1, MO1A, CR3, AB_528135, monoclonal, Human/Extracellular matrix proteins/Cluster Determinant antigens/Cell signaling/Cell adhesion/Cell markers/Stem cell, Immunohistochemistry/Immunofluorescence/Immunoprecipitation/Function Blocking/FACS

Antigen Molecular Weight :

Predicted: 127 kDa; Apparent: 170 kDa

Gene :

ITGAM

Alternate Gene Name(s) :

MAC-1, MO1A, CR3

Uniprot ID :

P11215

Antibody Registry ID :

AB_528135

Synonyms (Alternate Clone Names) :

LM2/1

Immunogen :

CD11b immunoprecipitated from Human granulocyte lysates + boost with Human live granulocytes.

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag8.6.5.3

Epitope Mapped :

Yes

Epitope Location or Sequence :

I domain (aa 148-332)

Epitope Map PubMed ID :

7679388

Recommended Applications :

FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation

Immunoblotting (legacy) :

Immunohistochemistry Pubmed IDs :

12111820

Immunofluorescence Pubmed IDs :

12390351

Western Blot Pubmed IDs :

2428876

Immunoprecipitation Pubmed IDs :

2428876 1967263 8945590 7679388 14769799 7592772

Function Blocking Pubmed IDs :

10080540 2428876 1967263 3519773 9862679 1723715 8945590 7679388 8409462

FACS Pubmed IDs :

2428876 7679388

Additional Information :

Mac-1 is a macrophage differentiation antigen and a marker for microglia.

DSHB Growth Medium :

RPMI

References (legacy) :

J. Immunol. 137, 2891-2900.

more info or order :

Developmental Studies Hybridoma Bank product webpage