LET-413 (lap1, Scribble) | Developmental Studies Hybridoma Bank LET413 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

LET-413 (lap1, Scribble)

catalog :

LET413

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

LET413

reactivity :

Caenorhabditis elegans

application :

western blot, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 3

Reference
Budirahardja Y, Doan T, Zaidel Bar R. Glycosyl phosphatidylinositol anchor biosynthesis is essential for maintaining epithelial integrity during Caenorhabditis elegans embryogenesis. PLoS Genet. 2015;11:e1005082 pubmed publisher
Cheng L, Li B, Wang Z. Level-Set Minimization of Potential Controlled Hadwiger Valuations for Molecular Solvation. J Comput Phys. 2010;229:8497-8510 pubmed
Hadwiger G, Dour S, Arur S, Fox P, Nonet M. A monoclonal antibody toolkit for C. elegans. PLoS ONE. 2010;5:e10161 pubmed publisher

product information

Internal ID :

6493

Name :

LET413

Depositor Name :

Nonet, M.L. / Hadwiger, G. / Dour, S.

Depositor Institution :

Washington University Medical School

Date Deposited :

2/16/10

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

LET-413 (lap1, Scribble)

Antigen Species :

C. elegans

Host Species :

mouse

Isotype :

MIgM

Isotype for catalog (legacy) :

IgM

Positive Tested Species Reactivity :

C. elegans

Species Tested (legacy) :

C. elegans

Initial Publication Pubmed ID :

20405020

Depositor Notes (Special Instructions) :

Works well on C. elegans whole mounts. Stains basolateral membranes.

Collections :

C. elegans

Search Keywords :

Nonet, M.L. / Hadwiger, G. / Dour, S., LET-413 (lap1, Scribble), C. elegans, MIgM, C. elegans, let-413, , AB_10571452, monoclonal, C. elegans, Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: 75 kDA and 77 kDa; Apparent: 76 kDa (and two faint bands at 110 and 200 kDa)

Gene :

let-413

Uniprot ID :

O61967

Antibody Registry ID :

AB_10571452

Synonyms (Alternate Clone Names) :

5C12

Immunogen :

Recombinant His6-tagged LET-413 (aa 460-606 of isoform a) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 460-606 of isoform a

Epitope Map PubMed ID :

20405020

Recommended Applications :

Immunofluorescence,Western Blot

Immunoblotting (legacy) :

good

Immunofluorescence Pubmed IDs :

20405020 25807459

Western Blot Pubmed IDs :

20405020

Additional Information :

The protein lap1 encoded by let-413 is a homolog of ERBIN, DENSIN-180 and SCRIB, an epithelial basolateral membrane protein, in the LAP family of proteins.

DSHB Growth Medium :

Iscove's

References (legacy) :

Hadwiger, G., Dour, S., Arur, S., Fox, P., and Nonet, M.L. (2010) A monoclonal antibody toolkit for C. elegans. PLoS One. 5(4):e10161-10115.

more info or order :

Developmental Studies Hybridoma Bank product webpage