tropomyosin, hTPM4 | Developmental Studies Hybridoma Bank LC24 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

tropomyosin, hTPM4

catalog :

LC24

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

LC24

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:150; loading ...; fig 2c western blot; human; 1:500; loading ...; fig 1c	Jiu Y, Peranen J, Schaible N, Cheng F, Eriksson J, Krishnan R, et al. Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA. J Cell Sci. 2017;130:892-902 pubmed publisher
	Schevzov G, Whittaker S, Fath T, Lin J, Gunning P. Tropomyosin isoforms and reagents. Bioarchitecture. 2011;1:135-164 pubmed
	Gallant C, Appel S, Graceffa P, Leavis P, Lin J, Gunning P, et al. Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells. Am J Physiol Cell Physiol. 2011;300:C1356-65 pubmed publisher
	Lin J, Eppinga R, Warren K, McCrae K. Human tropomyosin isoforms in the regulation of cytoskeleton functions. Adv Exp Med Biol. 2008;644:201-22 pubmed
	Nazeer K, Janech M, Lin J, Ryan K, Arthur J, Budisavljevic M. Changes in protein profiles during course of experimental glomerulonephritis. Am J Physiol Renal Physiol. 2009;296:F186-93 pubmed publisher
	Mirza Z, Sastri B, Lin J, Amenta P, Das K. Autoimmunity against human tropomyosin isoforms in ulcerative colitis: localization of specific human tropomyosin isoforms in the intestine and extraintestinal organs. Inflamm Bowel Dis. 2006;12:1036-43 pubmed
	O Hara S, Lin J. Accumulation of tropomyosin isoform 5 at the infection sites of host cells during Cryptosporidium invasion. Parasitol Res. 2006;99:45-54 pubmed
	Pawlak G, McGarvey T, Nguyen T, Tomaszewski J, Puthiyaveettil R, Malkowicz S, et al. Alterations in tropomyosin isoform expression in human transitional cell carcinoma of the urinary bladder. Int J Cancer. 2004;110:368-73 pubmed
	Ashworth S, Wean S, Campos S, Temm Grove C, Southgate E, Vrhovski B, et al. Renal ischemia induces tropomyosin dissociation-destabilizing microvilli microfilaments. Am J Physiol Renal Physiol. 2004;286:F988-96 pubmed
	DesMarais V, Ichetovkin I, Condeelis J, Hitchcock Degregori S. Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge. J Cell Sci. 2002;115:4649-60 pubmed
	Kesari K, Yoshizaki N, Geng X, Lin J, Das K. Externalization of tropomyosin isoform 5 in colon epithelial cells. Clin Exp Immunol. 1999;118:219-27 pubmed
	Geng X, Biancone L, Dai H, Lin J, Yoshizaki N, Dasgupta A, et al. Tropomyosin isoforms in intestinal mucosa: production of autoantibodies to tropomyosin isoforms in ulcerative colitis. Gastroenterology. 1998;114:912-22 pubmed
	Lin J, Warren K, Wamboldt D, Wang T, Lin J. Tropomyosin isoforms in nonmuscle cells. Int Rev Cytol. 1997;170:1-38 pubmed
	Pelham R, Lin J, Wang Y. A high molecular mass non-muscle tropomyosin isoform stimulates retrograde organelle transport. J Cell Sci. 1996;109 ( Pt 5):981-9 pubmed
	Warren K, Lin J, McDermott J, Lin J. Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis. J Cell Biol. 1995;129:697-708 pubmed

product information

Internal ID :

6142

Name :

LC24

Depositor Name :

Lin, J.J.-C.

Depositor Institution :

University of Iowa

Date Deposited :

7/17/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

tropomyosin, hTPM4

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human,Rodent

Species Tested (legacy) :

human and rodent non-muscle cells

Initial Publication Pubmed ID :

7730405

Depositor Notes (Special Instructions) :

Recognizes Human (hTM4) and rodent non-muscle tropomyosin.

Collections :

Cytoskeleton,Human,Skeletal muscle

Search Keywords :

Lin, J.J.-C., tropomyosin, hTPM4, Human, MIgG1, human/rodent, TPM4, , AB_10573119, monoclonal, Cytoskeletal elements/skeletal muscle/Human, Immunohistochemistry/Immunofluorescence/Western Blot/FACS

Antigen Molecular Weight :

Apparent: 36 and 30 kDa

Gene :

TPM4

Uniprot ID :

P67936

Antibody Registry ID :

AB_10573119

Immunogen :

Recombinant human tropomyosin isoform 5 and 4 fusion (hTM5/4) protein (N-terminal half of hTM5 and C-terminal half of hTM4).

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Yes

Epitope Location or Sequence :

C-terminal half of hTm4 (TPM4 exon 9d)

Epitope Map PubMed ID :

22069507

Recommended Applications :

FACS,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

17075344 16479376

Immunofluorescence Pubmed IDs :

22069507 16479376 19209824 12415009 8743945

Western Blot Pubmed IDs :

22069507 9558279 17075344 16479376 7730405 18987115 12415009 15095301 15075195 21289288

FACS Pubmed IDs :

10540182

Pubmed IDs :

9002235

Additional Information :

Cross reacts with Tm1 from TPM2.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 129, 697-708.; Int. Rev. Cytol. 170, 1-38.; Gastroenterology 114, 912-922.; Parasitol. Res. 99, 45-54.; Inflamm. Bowel Dis. 12, 1036-1043.; Adv. Exp. Med. Biol. 644, 201-222.; Am. J. Physiol. Renal Physiol. 296, F186-F193.

more info or order :

Developmental Studies Hybridoma Bank product webpage