tropomyosin, hTPM3 | Developmental Studies Hybridoma Bank LC1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

tropomyosin, hTPM3

catalog :

LC1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

LC1

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 15

Reference
Lees J, Ching Y, Adams D, Bach C, Samuel M, Kee A, et al. Tropomyosin regulates cell migration during skin wound healing. J Invest Dermatol. 2013;133:1330-9 pubmed publisher
Schevzov G, Whittaker S, Fath T, Lin J, Gunning P. Tropomyosin isoforms and reagents. Bioarchitecture. 2011;1:135-164 pubmed
Gallant C, Appel S, Graceffa P, Leavis P, Lin J, Gunning P, et al. Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells. Am J Physiol Cell Physiol. 2011;300:C1356-65 pubmed publisher
Ebert E, Geng X, Bajpai M, Pan Z, Tatar E, Das K. Antibody to tropomyosin isoform 5 and complement induce the lysis of colonocytes in ulcerative colitis. Am J Gastroenterol. 2009;104:2996-3003 pubmed publisher
Martin C, Gunning P. Isoform sorting of tropomyosins. Adv Exp Med Biol. 2008;644:187-200 pubmed
Eppinga R, Li Y, Lin J, Lin J. Tropomyosin and caldesmon regulate cytokinesis speed and membrane stability during cell division. Arch Biochem Biophys. 2006;456:161-74 pubmed
Vera C, Lao J, Hamelberg D, Sung L. Mapping the tropomyosin isoform 5 binding site on human erythrocyte tropomodulin: further insights into E-Tmod/TM5 interaction. Arch Biochem Biophys. 2005;444:130-8 pubmed
Schevzov G, Bryce N, Almonte Baldonado R, Joya J, Lin J, Hardeman E, et al. Specific features of neuronal size and shape are regulated by tropomyosin isoforms. Mol Biol Cell. 2005;16:3425-37 pubmed
Lin J, Geng X, Bhattacharya S, Yu J, Reiter R, Sastri B, et al. Isolation and sequencing of a novel tropomyosin isoform preferentially associated with colon cancer. Gastroenterology. 2002;123:152-62 pubmed
Vera C, Sood A, Gao K, Yee L, Lin J, Sung L. Tropomodulin-binding site mapped to residues 7-14 at the N-terminal heptad repeats of tropomyosin isoform 5. Arch Biochem Biophys. 2000;378:16-24 pubmed
Sung L, Gao K, Yee L, Temm Grove C, Helfman D, Lin J, et al. Tropomyosin isoform 5b is expressed in human erythrocytes: implications of tropomodulin-TM5 or tropomodulin-TM5b complexes in the protofilament and hexagonal organization of membrane skeletons. Blood. 2000;95:1473-80 pubmed
Kesari K, Yoshizaki N, Geng X, Lin J, Das K. Externalization of tropomyosin isoform 5 in colon epithelial cells. Clin Exp Immunol. 1999;118:219-27 pubmed
Geng X, Biancone L, Dai H, Lin J, Yoshizaki N, Dasgupta A, et al. Tropomyosin isoforms in intestinal mucosa: production of autoantibodies to tropomyosin isoforms in ulcerative colitis. Gastroenterology. 1998;114:912-22 pubmed
Lin J, Warren K, Wamboldt D, Wang T, Lin J. Tropomyosin isoforms in nonmuscle cells. Int Rev Cytol. 1997;170:1-38 pubmed
Warren K, Lin J, McDermott J, Lin J. Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis. J Cell Biol. 1995;129:697-708 pubmed

product information

Internal ID :

6137

Name :

LC1

Depositor Name :

Lin, J.J.-C.

Depositor Institution :

University of Iowa

Date Deposited :

7/17/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

tropomyosin, hTPM3

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Hamster,Human

Species Tested (legacy) :

human non-muscle, hamster (CHO) but not mouse

Initial Publication Pubmed ID :

7730405

Depositor Notes (Special Instructions) :

Recognizes Human non-muscle tropomyosin (hTM5), hamster (CHO) but not mouse.

Collections :

Cytoskeleton,Human,Skeletal muscle

Search Keywords :

Lin, J.J.-C., tropomyosin, hTPM3, Human, MIgG1, human/hamster, TPM3, , AB_10572389, monoclonal, Cytoskeletal elements/skeletal muscle/Human, Immunofluorescence/Immunoprecipitation/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Apparent: 32 kDa

Gene :

TPM3

Uniprot ID :

P06753

Antibody Registry ID :

AB_10572389

Immunogen :

Recombinant human tropomyosin isoform 5 and 4 fusion (hTM5/4) protein (N-terminal half of hTM5 and C-terminal half of hTM4).

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Yes

Epitope Location or Sequence :

First ten aa of the human TPM3 exon 1b product

Epitope Map PubMed ID :

22069507 10871039

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

19209823 19690525 16854366

Western Blot Pubmed IDs :

22069507 9558279 10540182 10871039 7730405 10871039 12105844 10666227 23303457 21289288

Immunoprecipitation Pubmed IDs :

16297372

Pubmed IDs :

9002235

Additional Information :

Doesn't cross-react with rat or mouse tropomyosin.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 129, 697-708.; Int. Rev. Cytol. 170, 1-38.; Gastroenterology 114, 912-922.; Clin. Exp. Immunol. 118, 219-227.; Blood 95, 1473-1480.; Arch. Biochem. Biophys. 378, 16-24.; Gastroenterology 123, 152-162.; Mol Biol. Cell 16, 3425-3437.; Arch. Biochem. Biophys. 456, 161-174.

more info or order :

Developmental Studies Hybridoma Bank product webpage