product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
laminin, murine parietal yolk sac
catalog :
LAM-B
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
LAM-B
reactivity :
mouse
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation
citations: 3
Reference
Johkura K, Cui L, Asanuma K, Okouchi Y, Ogiwara N, Sasaki K. Cytochemical and ultrastructural characterization of growing colonies of human embryonic stem cells. J Anat. 2004;205:247-55 pubmed
Albrechtsen R, Wewer U, Thorgeirsson S. De novo deposition of laminin-positive basement membrane in vitro by normal hepatocytes and during hepatocarcinogenesis. Hepatology. 1988;8:538-46 pubmed
Wewer U, Tichy D, Damjanov A, Paulsson M, Damjanov I. Distinct antigenic characteristics of murine parietal yolk sac laminin. Dev Biol. 1987;121:397-407 pubmed
product information
Internal ID :
1885
Name :
LAM-B
Depositor Name :
Damjanov, I.
Depositor Institution :
Kanas University Medical Center
Date Deposited :
11/3/92
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
laminin, murine parietal yolk sac
Antigen Species :
Mouse
Host Species :
rat
Isotype :
RIgG2a
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Mouse
Species Tested (legacy) :
mouse; not rat
Initial Publication Pubmed ID :
2438172
Depositor Notes (Special Instructions) :
Mouse laminin, yolk sac specific. This antibody reacts only with parietal yolk sac laminin, 6-18 day yolk sac of mouse; does not react with adult basement membrane or rat yolk sac.
Collections :
Cell adhesion,Cell signaling,Extracellular matrix proteins
Search Keywords :
Damjanov, I., laminin, murine parietal yolk sac, Mouse, RIgG2a, Mouse, , , AB_528340, monoclonal, Cell signaling/Extracellular matrix proteins/Cell adhesion, Immunofluorescence/Western Blot/Immunoprecipitation/ELISA
Antigen Molecular Weight :
Apparent: 200 kDa (beta and gamma) and 400 kDa (alpha)
Antibody Registry ID :
AB_528340
Immunogen :
Mouse yolk sac carcinoma cells derived from NF-1 teratocarcinoma.
Immunogen Sequence :
full length protein
Clonality :
Monoclonal
Myeloma Strain :
P3-X63-Ag-8.653
Epitope Mapped :
No
Recommended Applications :
ELISA,Immunofluorescence,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
Immunofluorescence Pubmed IDs :
2438172 15447684
Immunoprecipitation Pubmed IDs :
2438172
ELISA Pubmed IDs :
2438172
Pubmed IDs :
2453431
Additional Information :
Immunoblotting: LAM-B doesn't work well on denatured laminin.
DSHB Growth Medium :
Iscove's
References (legacy) :
Dev. Biol. 121, 397-407.; Acta Anatomica 146, 3-13.; Acta Anatomica 146, 223-233.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US