klarsicht, exon 9 | Developmental Studies Hybridoma Bank KLAR-M 4B5 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

klarsicht, exon 9

catalog :

KLAR-M 4B5

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

KLAR-M 4B5

reactivity :

fruit fly

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 7

Reference
Gaspar I, Yu Y, Cotton S, Kim D, Ephrussi A, Welte M. Klar ensures thermal robustness of oskar localization by restraining RNP motility. J Cell Biol. 2014;206:199-215 pubmed publisher
Kim D, Cotton S, Manna D, WELTE M. Novel isoforms of the transport regulator klar. PLoS ONE. 2013;8:e55070 pubmed publisher
Elhanany Tamir H, Yu Y, Shnayder M, Jain A, WELTE M, Volk T. Organelle positioning in muscles requires cooperation between two KASH proteins and microtubules. J Cell Biol. 2012;198:833-46 pubmed publisher
Yu Y, Li Z, Rizzo N, Einstein J, Welte M. Targeting the motor regulator Klar to lipid droplets. BMC Cell Biol. 2011;12:9 pubmed publisher
Shubeita G, Tran S, Xu J, Vershinin M, Cermelli S, Cotton S, et al. Consequences of motor copy number on the intracellular transport of kinesin-1-driven lipid droplets. Cell. 2008;135:1098-107 pubmed publisher
Meyer W, Schreiber S, Guo Y, Volkmann T, Welte M, Müller H. Overlapping functions of argonaute proteins in patterning and morphogenesis of Drosophila embryos. PLoS Genet. 2006;2:e134 pubmed
Guo Y, Jangi S, Welte M. Organelle-specific control of intracellular transport: distinctly targeted isoforms of the regulator Klar. Mol Biol Cell. 2005;16:1406-16 pubmed

product information

Internal ID :

6022

Name :

KLAR-M 4B5

Depositor Name :

Wieschaus, E.

Depositor Institution :

Princeton University

Date Deposited :

5/6/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

klarsicht, exon 9

Antigen Species :

Drosophila melanogaster

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila melanogaster

Species Tested (legacy) :

Drosophila melanogaster

Initial Publication Pubmed ID :

15647372

Depositor Notes (Special Instructions) :

mutant analysis indicates that it specifically recognizes Klar by western blotting and immunohistochemistry. Works well with heat-fixed tissues.

Collections :

Drosophila antigens

Search Keywords :

Wieschaus, E., klarsicht, exon 9, Drosophila melanogaster, MIgG1, Drosophila melanogaster , klar, CG17046, kls, marb, AB_10573114, monoclonal, Drosophila antigens, Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: multiple isoforms from 56 to 246 kDa; Apparent: major band above 250 kDa and multiple additional bands.

Gene :

klar

Alternate Gene Name(s) :

CG17046, kls, marb

Uniprot ID :

Q9W0Q4

Antibody Registry ID :

AB_10573114

Synonyms (Alternate Clone Names) :

KLAR-M

Immunogen :

Recombinant GST/Klarsicht (isoform A, aa 875-1169) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 875-1169 of isoform A

Epitope Map PubMed ID :

15647372

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

15647372 25049271 23457459 21349165 19070579 16934003

Western Blot Pubmed IDs :

15647372 25049271 23457459 22927463 21349165

Additional Information :

KLAR-M should recognize isoformsA, B, D, E, F, and H (not C, G, or I) [isoform nomenclature from FlyBase].

DSHB Growth Medium :

RPMI

References (legacy) :

Mol. Biol. Cell 16, 1406-1416.; PLos Genetics 2, 1224-1239.; Cell 135, 1098-1107.

more info or order :

Developmental Studies Hybridoma Bank product webpage