klarsicht, exon 18 | Developmental Studies Hybridoma Bank KLAR-C 9C10 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

klarsicht, exon 18

catalog :

KLAR-C 9C10

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

KLAR-C 9C10

reactivity :

fruit fly

application :

western blot, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 5

Reference
Myat M, Rashmi R, Manna D, Xu N, Patel U, Galiano M, et al. Drosophila KASH-domain protein Klarsicht regulates microtubule stability and integrin receptor localization during collective cell migration. Dev Biol. 2015;407:103-14 pubmed publisher
Gaspar I, Yu Y, Cotton S, Kim D, Ephrussi A, Welte M. Klar ensures thermal robustness of oskar localization by restraining RNP motility. J Cell Biol. 2014;206:199-215 pubmed publisher
Kim D, Cotton S, Manna D, WELTE M. Novel isoforms of the transport regulator klar. PLoS ONE. 2013;8:e55070 pubmed publisher
Technau M, Roth S. The Drosophila KASH domain proteins Msp-300 and Klarsicht and the SUN domain protein Klaroid have no essential function during oogenesis. Fly (Austin). 2008;2:82-91 pubmed
Guo Y, Jangi S, Welte M. Organelle-specific control of intracellular transport: distinctly targeted isoforms of the regulator Klar. Mol Biol Cell. 2005;16:1406-16 pubmed

product information

Internal ID :

6011

Name :

KLAR-C 9C10

Depositor Name :

Wieschaus, E.

Depositor Institution :

Princeton University

Date Deposited :

5/6/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

klarsicht, exon 18

Antigen Species :

Drosophila melanogaster

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila melanogaster

Species Tested (legacy) :

Drosophila melanogaster

Initial Publication Pubmed ID :

15647372

Depositor Notes (Special Instructions) :

mutant analysis indicates that it specifically recognizes Klar by western blotting and immunohistochemistry. Works both with heat-fixed and formaldehyde-fixed tissues.

Collections :

Drosophila antigens

Search Keywords :

Wieschaus, E., klarsicht, exon 18, Drosophila melanogaster, MIgG1, Drosophila melanogaster , klar, CG17046, kls, marb, AB_10584797, monoclonal, Drosophila antigens, Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: multiple isoforms from 56 to 246 kDa; Apparent: major band above 250 kDa and multiple additional bands.

Gene :

klar

Alternate Gene Name(s) :

CG17046, kls, marb

Uniprot ID :

Q9W0Q4

Antibody Registry ID :

AB_10584797

Synonyms (Alternate Clone Names) :

KLAR-C

Immunogen :

Recombinant GST/Klarsicht (isoform A, aa 1895-2262) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 2056-2202 of isoform A

Epitope Map PubMed ID :

15647372

Recommended Applications :

Immunofluorescence,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

15647372 25049271 23457459 18820478 31006649

Western Blot Pubmed IDs :

15647372 25049271 23457459

Pubmed IDs :

26247519

Additional Information :

KLAR-C should recognize isoforms A, B, C, E, F, G, and I (not D or H) [isoform nomenclature from FlyBase].

DSHB Growth Medium :

RPMI

References (legacy) :

Mol. Biol. Cell 16, 1406-1416.

more info or order :

Developmental Studies Hybridoma Bank product webpage