P granule abnormality protein 1 | Developmental Studies Hybridoma Bank K76 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

P granule abnormality protein 1

catalog :

K76

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

K76

reactivity :

roundworm

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; roundworm ; fig 7a	Manage K, Rogers A, Wallis D, Uebel C, Anderson D, Nguyen D, et al. A tudor domain protein, SIMR-1, promotes siRNA production at piRNA-targeted mRNAs in C. elegans. elife. 2020;9: pubmed publisher
	Wong M, Belew M, Kwieraga A, Nhan J, Michael W. Programmed DNA Breaks Activate the Germline Genome in Caenorhabditis elegans. Dev Cell. 2018;46:302-315.e5 pubmed publisher
	Lin L, Li Y, Yan L, Zhang G, Zhao Y, Zhang H. LSY-2 is essential for maintaining the germ-soma distinction in C. elegans. Protein Cell. 2015;6:599-609 pubmed publisher
	Strome S, Wood W. Generation of asymmetry and segregation of germ-line granules in early C. elegans embryos. Cell. 1983;35:15-25 pubmed
	Strome S. Asymmetric movements of cytoplasmic components in Caenorhabditis elegans zygotes. J Embryol Exp Morphol. 1986;97 Suppl:15-29 pubmed
	Hill D, Strome S. An analysis of the role of microfilaments in the establishment and maintenance of asymmetry in Caenorhabditis elegans zygotes. Dev Biol. 1988;125:75-84 pubmed
	Hill D, Strome S. Brief cytochalasin-induced disruption of microfilaments during a critical interval in 1-cell C. elegans embryos alters the partitioning of developmental instructions to the 2-cell embryo. Development. 1990;108:159-72 pubmed
	Capowski E, Martin P, Garvin C, Strome S. Identification of grandchildless loci whose products are required for normal germ-line development in the nematode Caenorhabditis elegans. Genetics. 1991;129:1061-72 pubmed

product information

Internal ID :

473

Name :

K76

Depositor Name :

Strome, S. / Wood, W.B.

Depositor Institution :

University of California Santa Cruz/ University of Colorado

Date Deposited :

5/18/94

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

P granule abnormality protein 1

Antigen Species :

C. elegans

Host Species :

mouse

Isotype :

MIgM

Isotype for catalog (legacy) :

IgM

Positive Tested Species Reactivity :

C. elegans

Species Tested (legacy) :

does not immunostain germ granules in other nematodes (Caenorhabditis briggsae, Panagrellus redivivus, Ascaris) or in Drosophila. It may faintly stain germ granules in Xenopus

Initial Publication Pubmed ID :

6684994

Depositor Notes (Special Instructions) :

In adult hermaphrodites, K76 stains both germline granules and spermatheca.

Collections :

C. elegans,Cell markers,Cell signaling

Search Keywords :

Susan Strome & William B. Wood, P granule abnormality protein 1, C. elegans, MIgM, C. elegans, pgl-1 , ZK381.4 , AB_531836, Monoclonal, C. elegans, Immunofluorescence/Western blot/Immunoprecipitation

Antigen Molecular Weight :

40 kDa

Gene :

pgl-1

Alternate Gene Name(s) :

ZK381.4

Uniprot ID :

Q9TZQ3

Antibody Registry ID :

AB_531836

Immunogen :

C. elegans embryo homogenate

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunofluorescence Pubmed IDs :

6684994 3625110 3275427 2190787 1783292 26050091 30086301

Western Blot Pubmed IDs :

6684994

Immunoprecipitation Pubmed IDs :

6684994

Additional Information :

RRID:AB_531836

DSHB Growth Medium :

RPMI

References (legacy) :

Cell 35, 15-25.; "Time, Space, and Pattern in Embryonic Development", Jeffrey,W.R., & Raff, R.A. pp 221-239.; "Molecular Aspects of Early Development", Malacinski,G.M. & Klein, W.H. pp 141-165.; “Molecular Biology of Development”, Davidson, E.H., and Firtel, R.A. pp 37-49.; J. Embryol. Exp. Morph. 97, 15-29.; “Gametogenesis and the Early Embryo”, Gall, J.G. pp. 77-95.; Dev. Biol. 125, 75-84.; Development 108, 159-172.; Genetics 129, 1061-1072.

more info or order :

Developmental Studies Hybridoma Bank product webpage