troponin T, fast skeletal muscle specific | Developmental Studies Hybridoma Bank JLT12 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

troponin T, fast skeletal muscle specific

catalog :

JLT12

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

JLT12

reactivity :

rabbit

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 10

Reference
Tikunova S, Belevych N, Doan K, Reiser P. Desensitizing mouse cardiac troponin C to calcium converts slow muscle towards a fast muscle phenotype. J Physiol. 2018;596:4651-4663 pubmed publisher
Wang G, Biswas A, Ma W, Kandpal M, Coker C, Grandgenett P, et al. Metastatic cancers promote cachexia through ZIP14 upregulation in skeletal muscle. Nat Med. 2018;24:770-781 pubmed publisher
Zhang C, Pietras K, Sferrazza G, Jia P, Athauda G, Rueda de Leon E, et al. Molecular and immunohistochemical analyses of cardiac troponin T during cardiac development in the Mexican axolotl, Ambystoma mexicanum. J Cell Biochem. 2007;100:1-15 pubmed
Green S, Glover R, Krumm P, Winters T, Atkins D. A soluble neuronal factor alters contractile function of ventricular myocytes without effect on troponin T isoform expression. Cardiovasc Res. 1999;41:200-11 pubmed
Briggs M, Schachat F. Origin of fetal troponin T: developmentally regulated splicing of a new exon in the fast troponin T gene. Dev Biol. 1993;158:503-9 pubmed
Warren K, Lin J. Forced expression and assembly of rat cardiac troponin T isoforms in cultured muscle and nonmuscle cells. J Muscle Res Cell Motil. 1993;14:619-32 pubmed
Moore G, Briggs M, Schachat F. Patterns of troponin T expression in mammalian fast, slow and promiscuous muscle fibres. J Muscle Res Cell Motil. 1987;8:13-22 pubmed
Briggs M, Jacoby J, Davidowitz J, Schachat F. Expression of a novel combination of fast and slow troponin T isoforms in rabbit extraocular muscles. J Muscle Res Cell Motil. 1988;9:241-7 pubmed
Briggs M, Lin J, Schachat F. The extent of amino-terminal heterogeneity in rabbit fast skeletal muscle troponin T. J Muscle Res Cell Motil. 1987;8:1-12 pubmed
Briggs M, McGinnis H, Schachat F. Transitions from fetal to fast troponin T isoforms are coordinated with changes in tropomyosin and alpha-actinin isoforms in developing rabbit skeletal muscle. Dev Biol. 1990;140:253-60 pubmed

product information

Internal ID :

9391

Name :

JLT12

Depositor Name :

Lin, J.J.-C.

Depositor Institution :

University of Iowa

Date Deposited :

3/20/14

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

troponin T, fast skeletal muscle specific

Antigen Species :

Rabbit

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Axolotl,Bovine,Broad species,Chicken,Human,Rabbit,Rat

Species Tested (legacy) :

broad species specificity

Initial Publication Pubmed ID :

2439538

Depositor Notes (Special Instructions) :

Paraformaldehyde fixation for immunostaining is recommended

Collections :

Human,Skeletal muscle

Search Keywords :

Lin, J.J.-C., troponin T, fast skeletal muscle specific, Rabbit, MIgG1, Broad species/rabbit/bovine/chicken/human/rat/Axolotl, TNNT3, TnTf, fTnT, AB_2618103, monoclonal, Human/Skeletal muscle, Western Blot/Immunoprecipitation/Immunofluorescence

Antigen Molecular Weight :

Multiple isoforms; Predicted: 29 to 33 kDa

Gene :

TNNT3

Alternate Gene Name(s) :

TnTf, fTnT

Uniprot ID :

P02641

Antibody Registry ID :

AB_2618103

Immunogen :

Skeletal muscle troponin T (TnT)

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 71-151 of TnT2f

Epitope Map PubMed ID :

2439538

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

8126222

Western Blot Pubmed IDs :

2439538 8126222 3410960 8344466 2373251 3597761 10325967 16888779 31411733

Additional Information :

JLT12 recognizes all Troponin T, fast skeletal muscle isoforms and doesn't recognize slow isoforms. This antibody was initially characterized in Lin et al. [Lin, J.J.-C., Feramisco, J.R., Blose, S.H., and Matsumura, F. (1984). Monoclonal antibodies to cytoskeletal proteins. In Monoclonal Antibodies and Hybridomas: Progress and Applications. (eds. R.H. Kennett, T.J. McKearn, and K.B. Bechtol) pp. 119-151, Plenum Press, New York].

DSHB Growth Medium :

RPMI

References (legacy) :

Lin, J.J.-C., Feramisco, J.R., Blose, S.H., and Matsumura, F. (1984). Monoclonal antibodies to cytoskeletal proteins. In Monoclonal Antibodies and Hybridomas: Progress and Applications. (eds. R.H. Kennett, T.J. McKearn, and K.B. Bechtol) pp. 119-151, Plenum Press, New York.; J. Muscle Res. Cell. Motil. 8, 1-12.; J. Biol. Chem. 263, 7309-7315.

more info or order :

Developmental Studies Hybridoma Bank product webpage