alpha sarcoglycan | Developmental Studies Hybridoma Bank IVD3(1)A9 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

alpha sarcoglycan

catalog :

IVD3(1)A9

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

IVD3(1)A9

reactivity :

mouse, rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 36

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:100; fig 6	Vanhoutte D, Schips T, Kwong J, Davis J, Tjondrokoesoemo A, Brody M, et al. Thrombospondin expression in myofibers stabilizes muscle membranes. elife. 2016;5: pubmed publisher
immunohistochemistry; mouse; 1:100; fig 5 western blot; mouse; 1:500; fig 5, 6	Tjondrokoesoemo A, Schips T, Kanisicak O, Sargent M, Molkentin J. Genetic overexpression of Serpina3n attenuates muscular dystrophy in mice. Hum Mol Genet. 2016;25:1192-202 pubmed publisher
immunocytochemistry; mouse; 1:100; loading ...; fig 7s western blot; mouse; 1:100; loading ...; fig 7a	Van Ry P, Wuebbles R, Key M, Burkin D. Galectin-1 Protein Therapy Prevents Pathology and Improves Muscle Function in the mdx Mouse Model of Duchenne Muscular Dystrophy. Mol Ther. 2015;23:1285-1297 pubmed publisher
immunohistochemistry - frozen section; mouse	Can T, Faas L, Ashford D, Dowle A, Thomas J, O Toole P, et al. Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections. Proteome Sci. 2014;12:25 pubmed publisher
	Sylow L, Nielsen I, Kleinert M, Møller L, Ploug T, Schjerling P, et al. Rac1 governs exercise-stimulated glucose uptake in skeletal muscle through regulation of GLUT4 translocation in mice. J Physiol. 2016;594:4997-5008 pubmed publisher
	Larcher T, Lafoux A, Tesson L, Remy S, Thepenier V, FranÃ§ois V, et al. Characterization of dystrophin deficient rats: a new model for Duchenne muscular dystrophy. PLoS ONE. 2014;9:e110371 pubmed publisher
	Bornø A, Ploug T, Bune L, Rosenmeier J, Thaning P. Purinergic receptors expressed in human skeletal muscle fibres. Purinergic Signal. 2012;8:255-64 pubmed publisher
	Bueno M, Moreira E, Vainzof M, Chamberlain J, Marie S, Pereira L, et al. A common missense mutation in the adhalin gene in three unrelated Brazilian families with a relatively mild form of autosomal recessive limb-girdle muscular dystrophy. Hum Mol Genet. 1995;4:1163-7 pubmed
	Ohlendieck K, Matsumura K, Ionasescu V, Towbin J, Bosch E, Weinstein S, et al. Duchenne muscular dystrophy: deficiency of dystrophin-associated proteins in the sarcolemma. Neurology. 1993;43:795-800 pubmed
	Klietsch R, Ervasti J, Arnold W, Campbell K, Jorgensen A. Dystrophin-glycoprotein complex and laminin colocalize to the sarcolemma and transverse tubules of cardiac muscle. Circ Res. 1993;72:349-60 pubmed
	Matsumura K, Tome F, Ionasescu V, Ervasti J, Anderson R, Romero N, et al. Deficiency of dystrophin-associated proteins in Duchenne muscular dystrophy patients lacking COOH-terminal domains of dystrophin. J Clin Invest. 1993;92:866-71 pubmed
	Matsumura K, Nonaka I, Tome F, Arahata K, Collin H, Leturcq F, et al. Mild deficiency of dystrophin-associated proteins in Becker muscular dystrophy patients having in-frame deletions in the rod domain of dystrophin. Am J Hum Genet. 1993;53:409-16 pubmed
	Matsumura K, Burghes A, Mora M, Tome F, Morandi L, Cornello F, et al. Immunohistochemical analysis of dystrophin-associated proteins in Becker/Duchenne muscular dystrophy with huge in-frame deletions in the NH2-terminal and rod domains of dystrophin. J Clin Invest. 1994;93:99-105 pubmed
	Matsumura K, Yamada H, Shimizu T, Campbell K. Differential expression of dystrophin, utrophin and dystrophin-associated proteins in peripheral nerve. FEBS Lett. 1993;334:281-5 pubmed
	Azibi K, Bachner L, Beckmann J, Matsumura K, Hamouda E, Chaouch M, et al. Severe childhood autosomal recessive muscular dystrophy with the deficiency of the 50 kDa dystrophin-associated glycoprotein maps to chromosome 13q12. Hum Mol Genet. 1993;2:1423-8 pubmed
	Campanelli J, Roberds S, Campbell K, Scheller R. A role for dystrophin-associated glycoproteins and utrophin in agrin-induced AChR clustering. Cell. 1994;77:663-74 pubmed
	Sunada Y, Bernier S, Kozak C, Yamada Y, Campbell K. Deficiency of merosin in dystrophic dy mice and genetic linkage of laminin M chain gene to dy locus. J Biol Chem. 1994;269:13729-32 pubmed
	Matsumura K, Nonaka I, Campbell K. Abnormal expression of dystrophin-associated proteins in Fukuyama-type congenital muscular dystrophy. Lancet. 1993;341:521-2 pubmed
	Zatz M, Matsumura K, Vainzof M, Passos Bueno M, Pavanello R, Marie S, et al. Assessment of the 50-kDa dystrophin-associated glycoprotein in Brazilian patients with severe childhood autosomal recessive muscular dystrophy. J Neurol Sci. 1994;123:122-8 pubmed
	Fardeau M, Matsumura K, Tome F, Collin H, Leturcq F, Kaplan J, et al. Deficiency of the 50 kDa dystrophin associated glycoprotein (adhalin) in severe autosomal recessive muscular dystrophies in children native from European countries. C R Acad Sci III. 1993;316:799-804 pubmed
	Higuchi I, Yamada H, Fukunaga H, Iwaki H, Okubo R, Nakagawa M, et al. Abnormal expression of laminin suggests disturbance of sarcolemma-extracellular matrix interaction in Japanese patients with autosomal recessive muscular dystrophy deficient in adhalin. J Clin Invest. 1994;94:601-6 pubmed
	Sewry C, Sansome A, Matsumura K, Campbell K, Dubowitz V. Deficiency of the 50 kDa dystrophin-associated glycoprotein and abnormal expression of utrophin in two south Asian cousins with variable expression of severe childhood autosomal recessive muscular dystrophy. Neuromuscul Disord. 1994;4:121-9 pubmed
	Tome F, Evangelista T, Leclerc A, Sunada Y, Manole E, Estournet B, et al. Congenital muscular dystrophy with merosin deficiency. C R Acad Sci III. 1994;317:351-7 pubmed
	Romero N, Tome F, Leturcq F, el Kerch F, Azibi K, Bachner L, et al. Genetic heterogeneity of severe childhood autosomal recessive muscular dystrophy with adhalin (50 kDa dystrophin-associated glycoprotein) deficiency. C R Acad Sci III. 1994;317:70-6 pubmed
	Sewry C, Matsumura K, Campbell K, Dubowitz V. Expression of dystrophin-associated glycoproteins and utrophin in carriers of Duchenne muscular dystrophy. Neuromuscul Disord. 1994;4:401-9 pubmed
	Yamada H, Tome F, Higuchi I, Kawai H, Azibi K, Chaouch M, et al. Laminin abnormality in severe childhood autosomal recessive muscular dystrophy. Lab Invest. 1995;72:715-22 pubmed
	Kawai H, Akaike M, Endo T, Adachi K, Inui T, Mitsui T, et al. Adhalin gene mutations in patients with autosomal recessive childhood onset muscular dystrophy with adhalin deficiency. J Clin Invest. 1995;96:1202-7 pubmed
	Vater R, Harris J, Anderson V, Roberds S, Campbell K, Cullen M. The expression of dystrophin-associated glycoproteins during skeletal muscle degeneration and regeneration. An immunofluorescence study. J Neuropathol Exp Neurol. 1995;54:557-69 pubmed
	Lim L, Duclos F, Broux O, Bourg N, Sunada Y, Allamand V, et al. Beta-sarcoglycan: characterization and role in limb-girdle muscular dystrophy linked to 4q12. Nat Genet. 1995;11:257-65 pubmed
	Matsumura K, Tome F, Collin H, Leturcq F, Jeanpierre M, Kaplan J, et al. Expression of dystrophin-associated proteins in dystrophin-positive muscle fibers (revertants) in Duchenne muscular dystrophy. Neuromuscul Disord. 1994;4:115-20 pubmed
	Sunada Y, Edgar T, Lotz B, Rust R, Campbell K. Merosin-negative congenital muscular dystrophy associated with extensive brain abnormalities. Neurology. 1995;45:2084-9 pubmed
	Jorgensen A, Arnold W, Shen A, Yuan S, Gaver M, Campbell K. Identification of novel proteins unique to either transverse tubules (TS28) or the sarcolemma (SL50) in rabbit skeletal muscle. J Cell Biol. 1990;110:1173-85 pubmed
	Ohlendieck K, Campbell K. Dystrophin constitutes 5% of membrane cytoskeleton in skeletal muscle. FEBS Lett. 1991;283:230-4 pubmed
	Ohlendieck K, Ervasti J, Snook J, Campbell K. Dystrophin-glycoprotein complex is highly enriched in isolated skeletal muscle sarcolemma. J Cell Biol. 1991;112:135-48 pubmed
	Ohlendieck K, Ervasti J, Matsumura K, Kahl S, Leveille C, Campbell K. Dystrophin-related protein is localized to neuromuscular junctions of adult skeletal muscle. Neuron. 1991;7:499-508 pubmed
	Matsumura K, Tome F, Collin H, Azibi K, Chaouch M, Kaplan J, et al. Deficiency of the 50K dystrophin-associated glycoprotein in severe childhood autosomal recessive muscular dystrophy. Nature. 1992;359:320-2 pubmed

product information

Internal ID :

1675

Name :

IVD3(1)A9

Depositor Name :

Campbell, K.P.

Depositor Institution :

University of Iowa

Date Deposited :

4/26/04

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

alpha sarcoglycan

Antigen Species :

Rabbit

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Canine,Human,Mouse,Rabbit,Rat,Sheep

Species Tested (legacy) :

rabbit, mouse, human

Initial Publication Pubmed ID :

2157716

Collections :

Cell signaling,Cytoskeleton,Extracellular matrix proteins,Human,Muscular dystrophy,Skeletal muscle

Search Keywords :

Campbell, K.P., alpha sarcoglycan, Rabbit, MIgG1, kappa light chain, Rabbit/Mouse/Human/Sheep/Canine/Rat, SGCA, ADL, DAG2, 50DAG, DMDA2, LGMD2D, SCARMD1, adhalin, AB_2185657, monoclonal, Cell signaling/Cytoskeletal elements/Extracellular matrix proteins/Human/Muscular dystrophy/Skeletal muscle, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 40 kDa; Apparent: 50 kDa

Gene :

SGCA

Alternate Gene Name(s) :

ADL, DAG2, 50DAG, DMDA2, LGMD2D, SCARMD1, adhalin

Uniprot ID :

Q28686

Antibody Registry ID :

AB_2185657

Synonyms (Alternate Clone Names) :

IVD31

Immunogen :

Isolated membranes from skeletal muscle.

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS-1

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

25310701

Immunofluorescence Pubmed IDs :

2157716 2157716 1986002 1654951 1406935 8418988 8094772 8469343 8328458 8349821 8242065 8243633 8282827 7516752 7987694 8064304 8040315 8205616 7881285 7783429 8528203 7602329 7657792 7581448 7501163 27061726 22052557 25071420 31379197

Western Blot Pubmed IDs :

2157716 2157716 1986002 2044761 8418988 8243633 26050991

Immunoprecipitation Pubmed IDs :

2157716 8188645

Pubmed IDs :

8044705 8000914 8012192

Additional Information :

The alpha sarcoglican is a component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.This antibody doesn't work on canine western blots. IVD3(1)A9 works under nonreducing conditions (and not under reducing conditions) for western blotting. One reference states that the epitope is located in the C-terminal region [PMID: 8040315]. RRID:AB_2185657

DSHB Growth Medium :

RPMI

References (legacy) :

J. Cell. Biol. 110, 1173-1185.; Nature 345, 315-319.; In: Frontiers of Muscle Research, eds. E. Ozawa, T. Masaki, and Y. Nabeshima. pp. 321-340.; J. Cell Biol. 112, 135-148.; FEBS Lett. 283, 230-234.; Neuron 7, 499-508.; Nature 359, 320-322.; Circ. Res. 72, 349-360.; Lancet 341, 521-522.; Neurology 43, 795-800.; Am. J. Hum. Genet. 53, 409-416.; J. Clin. Invest. 92, 866-871.; Hum. Mol. Genet. 2, 1423-1428.; C.R. Acad. Sci. Paris 316, 799-804.; FEBS Lett. 334, 281-285.; J. Clin. Invest. 93, 99-105.; Neuromuscular Disord. 4, 115-120.; C.R. Acad. Sci. Paris, Sciences de la Vie/Life Sciences 317, 70-76.; C.R. Acad. Sci. Paris, Sciences de la Vie/Life Sciences 317, 351-357.; Neuromuscular Disord. 4, 121-129.; J. Biol. Chem. 269, 13729-13732.; J. Neurol. Sci. 123, 122-128.; J. Clin. Invest. 94, 601-606.; Cell 77, 663-674.; FEBS Lett. 350, 173-176.; Neuromuscular Disord. 4, 401-409.; Nat. Genet. 8, 340-344.; Lab. Invest. 72, 715-722.; Hum. Mol. Genet. 4, 1163-1168.; J. Neuropathol. Exp. Neurol. 54, 557-569.; J. Clin. Invest. 96, 1202-1207.; Nat. Genet. 11, 257-265.; Neurology 45, 2084-2089.

more info or order :

Developmental Studies Hybridoma Bank product webpage