product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Collagen type II
catalog :
II-II6B3
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
II-II6B3
reactivity :
human, mouse, rat, dog, chicken, cow, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 104
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; zebrafish ; 1:100; loading ...; fig 2
Schulz A, Brendler J, Blaschuk O, Landgraf K, Krueger M, Ricken A. Non-pathological Chondrogenic Features of Valve Interstitial Cells in Normal Adult Zebrafish. J Histochem Cytochem. 2019;67:361-373 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 4a
Bartolomeo R, Cinque L, De Leonibus C, Forrester A, Salzano A, Monfregola J, et al. mTORC1 hyperactivation arrests bone growth in lysosomal storage disorders by suppressing autophagy. J Clin Invest. 2017;127:3717-3729 pubmed publisher
  • immunohistochemistry - paraffin section; human; loading ...; fig 3b
Diaz Romero J, Kürsener S, Kohl S, Nesic D. S100B?+?A1 CELISA: A Novel Potency Assay and Screening Tool for Redifferentiation Stimuli of Human Articular Chondrocytes. J Cell Physiol. 2017;232:1559-1570 pubmed publisher
  • immunohistochemistry; human; fig 6
Anderson D, Markway B, Bond D, McCarthy H, Johnstone B. Responses to altered oxygen tension are distinct between human stem cells of high and low chondrogenic capacity. Stem Cell Res Ther. 2016;7:154 pubmed
  • immunohistochemistry; zebrafish ; fig 3r
Masselink W, Cole N, Fényes F, Berger S, Sonntag C, Wood A, et al. A somitic contribution to the apical ectodermal ridge is essential for fin formation. Nature. 2016;535:542-6 pubmed
  • immunohistochemistry - paraffin section; mouse; 0.02 ug/ml; fig 2
  • immunohistochemistry - paraffin section; dog; 0.02 ug/ml; fig 7
  • immunohistochemistry - paraffin section; human; 0.4 ug/ml; fig 7
Bach F, Zhang Y, Miranda Bedate A, Verdonschot L, Bergknut N, Creemers L, et al. Increased caveolin-1 in intervertebral disc degeneration facilitates repair. Arthritis Res Ther. 2016;18:59 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 7
Antunes J, Tsaryk R, Gonçalves R, Pereira C, Landes C, Brochhausen C, et al. Poly(γ-Glutamic Acid) as an Exogenous Promoter of Chondrogenic Differentiation of Human Mesenchymal Stem/Stromal Cells. Tissue Eng Part A. 2015;21:1869-85 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 4
Narcisi R, Cleary M, Brama P, Hoogduijn M, Tüysüz N, ten Berge D, et al. Long-term expansion, enhanced chondrogenic potential, and suppression of endochondral ossification of adult human MSCs via WNT signaling modulation. Stem Cell Reports. 2015;4:459-72 pubmed publisher
  • immunocytochemistry; human; 1:200
Schrobback K, Klein T, Woodfield T. The importance of connexin hemichannels during chondroprogenitor cell differentiation in hydrogel versus microtissue culture models. Tissue Eng Part A. 2015;21:1785-94 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:20
Bertolo A, Hafner S, Taddei A, Baur M, Pötzel T, Steffen F, et al. Injectable microcarriers as human mesenchymal stem cell support and their application for cartilage and degenerated intervertebral disc repair. Eur Cell Mater. 2015;29:70-80; discujssion 80-1 pubmed
  • immunohistochemistry; cow; 1:200
Neu C, Novak T, Gilliland K, Marshall P, Calve S. Optical clearing in collagen- and proteoglycan-rich osteochondral tissues. Osteoarthritis Cartilage. 2015;23:405-13 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:200
Levett P, Hutmacher D, Malda J, Klein T. Hyaluronic acid enhances the mechanical properties of tissue-engineered cartilage constructs. PLoS ONE. 2014;9:e113216 pubmed publisher
  • immunohistochemistry; chicken
Botelho J, Ossa Fuentes L, Soto Acuña S, Smith Paredes D, Núñez León D, Salinas Saavedra M, et al. New developmental evidence clarifies the evolution of wrist bones in the dinosaur-bird transition. PLoS Biol. 2014;12:e1001957 pubmed publisher
  • immunohistochemistry; human; 1:40
  • western blot; human; 1:500
Capossela S, Schlafli P, Bertolo A, Janner T, Stadler B, Pötzel T, et al. Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins. Eur Cell Mater. 2014;27:251-63; discussion 263 pubmed
  • immunohistochemistry - paraffin section; human
Pei M, Li J, Zhang Y, Liu G, Wei L, Zhang Y. Expansion on a matrix deposited by nonchondrogenic urine stem cells strengthens the chondrogenic capacity of repeated-passage bone marrow stromal cells. Cell Tissue Res. 2014;356:391-403 pubmed publisher
  • immunohistochemistry; human; 1:200; fig 3s
Hesami P, Holzapfel B, Taubenberger A, Roudier M, Fazli L, Sieh S, et al. A humanized tissue-engineered in vivo model to dissect interactions between human prostate cancer cells and human bone. Clin Exp Metastasis. 2014;31:435-46 pubmed publisher
  • immunohistochemistry - paraffin section; human; 2 ug/mL
Hasegawa A, Nakahara H, Kinoshita M, Asahara H, Koziol J, Lotz M. Cellular and extracellular matrix changes in anterior cruciate ligaments during human knee aging and osteoarthritis. Arthritis Res Ther. 2013;15:R29 pubmed publisher
  • immunohistochemistry - paraffin section; human
Cheng N, Estes B, Young T, Guilak F. Genipin-crosslinked cartilage-derived matrix as a scaffold for human adipose-derived stem cell chondrogenesis. Tissue Eng Part A. 2013;19:484-96 pubmed publisher
Hagmeijer M, Vonk L, Fenu M, van Keep Y, Krych A, Saris D. Meniscus regeneration combining meniscus and mesenchymal stromal cells in a degradable meniscus implant: an in vitro study. Eur Cell Mater. 2019;38:51-62 pubmed publisher
Lolli A, Sivasubramaniyan K, Vainieri M, Oieni J, Kops N, Yayon A, et al. Hydrogel-based delivery of antimiR-221 enhances cartilage regeneration by endogenous cells. J Control Release. 2019;309:220-230 pubmed publisher
Schlumberger M, Schuster P, Bülow H, Mayer P, Eichinger M, Richter J. Arthroscopic autologous chondrocyte implantation in the knee with an in situ crosslinking matrix: minimum 4-year clinical results of 15 cases and 1 histological evaluation. Arch Orthop Trauma Surg. 2019;139:1607-1615 pubmed publisher
von Bomhard A, Elsaesser A, Riepl R, Pippich K, Faust J, Schwarz S, et al. Cartilage regeneration using decellularized cartilage matrix: Long-term comparison of subcutaneous and intranasal placement in a rabbit model. J Craniomaxillofac Surg. 2019;47:682-694 pubmed publisher
Kim M, Steinberg D, Burdick J, Mauck R. Extracellular vesicles mediate improved functional outcomes in engineered cartilage produced from MSC/chondrocyte cocultures. Proc Natl Acad Sci U S A. 2019;116:1569-1578 pubmed publisher
Chen S, Xu Z, Shao J, Fu P, Wu H. MicroRNA-218 promotes early chondrogenesis of mesenchymal stem cells and inhibits later chondrocyte maturation. BMC Biotechnol. 2019;19:6 pubmed publisher
Lindberg G, Longoni A, Lim K, Rosenberg A, Hooper G, Gawlitta D, et al. Intact vitreous humor as a potential extracellular matrix hydrogel for cartilage tissue engineering applications. Acta Biomater. 2019;85:117-130 pubmed publisher
Chagraoui H, Kristiansen M, Ruiz J, Serra Barros A, Richter J, Hall Ponsele E, et al. SCL/TAL1 cooperates with Polycomb RYBP-PRC1 to suppress alternative lineages in blood-fated cells. Nat Commun. 2018;9:5375 pubmed publisher
Ferreira C, Xia Z, Clément A, Parry D, Davids M, Taylan F, et al. A Recurrent De Novo Heterozygous COG4 Substitution Leads to Saul-Wilson Syndrome, Disrupted Vesicular Trafficking, and Altered Proteoglycan Glycosylation. Am J Hum Genet. 2018;103:553-567 pubmed publisher
Alvarez Garcia O, Matsuzaki T, Olmer M, Miyata K, Mokuda S, Sakai D, et al. FOXO are required for intervertebral disk homeostasis during aging and their deficiency promotes disk degeneration. Aging Cell. 2018;17:e12800 pubmed publisher
Lalonde R, Akimenko M. Effects of fin fold mesenchyme ablation on fin development in zebrafish. PLoS ONE. 2018;13:e0192500 pubmed publisher
LeBert D, Squirrell J, Freisinger C, Rindy J, Golenberg N, Frecentese G, et al. Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair. elife. 2018;7: pubmed publisher
Palade J, Djordjevic D, Hutchins E, George R, Cornelius J, Rawls A, et al. Identification of satellite cells from anole lizard skeletal muscle and demonstration of expanded musculoskeletal potential. Dev Biol. 2018;433:344-356 pubmed publisher
Li C, Zheng Z, Jiang J, Jiang W, Lee K, Berthiaume E, et al. Neural EGFL-Like 1 Regulates Cartilage Maturation through Runt-Related Transcription Factor 3-Mediated Indian Hedgehog Signaling. Am J Pathol. 2018;188:392-403 pubmed publisher
Brunt L, Begg K, Kague E, Cross S, Hammond C. Wnt signalling controls the response to mechanical loading during zebrafish joint development. Development. 2017;144:2798-2809 pubmed publisher
de Kroon L, Narcisi R, van den Akker G, Vitters E, Blaney Davidson E, van Osch G, et al. SMAD3 and SMAD4 have a more dominant role than SMAD2 in TGFβ-induced chondrogenic differentiation of bone marrow-derived mesenchymal stem cells. Sci Rep. 2017;7:43164 pubmed publisher
Mouser V, Abbadessa A, Levato R, Hennink W, Vermonden T, Gawlitta D, et al. Development of a thermosensitive HAMA-containing bio-ink for the fabrication of composite cartilage repair constructs. Biofabrication. 2017;9:015026 pubmed publisher
Chu J, Dai M, Wang Y, Tian F, Song H, Xiao Y, et al. Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. BMC Musculoskelet Disord. 2017;18:78 pubmed publisher
Moutos F, Glass K, Compton S, Ross A, Gersbach C, Guilak F, et al. Anatomically shaped tissue-engineered cartilage with tunable and inducible anticytokine delivery for biological joint resurfacing. Proc Natl Acad Sci U S A. 2016;113:E4513-22 pubmed publisher
Mouser V, Melchels F, Visser J, Dhert W, Gawlitta D, Malda J. Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting. Biofabrication. 2016;8:035003 pubmed publisher
Naranda J, Sušec M, Maver U, Gradisnik L, Gorenjak M, Vukasović A, et al. Polyester type polyHIPE scaffolds with an interconnected porous structure for cartilage regeneration. Sci Rep. 2016;6:28695 pubmed publisher
Shwartz Y, Viukov S, Krief S, Zelzer E. Joint Development Involves a Continuous Influx of Gdf5-Positive Cells. Cell Rep. 2016;15:2577-87 pubmed publisher
Abbadessa A, Mouser V, Blokzijl M, Gawlitta D, Dhert W, Hennink W, et al. A Synthetic Thermosensitive Hydrogel for Cartilage Bioprinting and Its Biofunctionalization with Polysaccharides. Biomacromolecules. 2016;17:2137-2147 pubmed publisher
Kean T, Mera H, Whitney G, Mackay D, Awadallah A, Fernandes R, et al. Disparate response of articular- and auricular-derived chondrocytes to oxygen tension. Connect Tissue Res. 2016;57:319-33 pubmed publisher
Paul S, Schindler S, Giovannone D, de Millo Terrazzani A, Mariani F, Crump J. Ihha induces hybrid cartilage-bone cells during zebrafish jawbone regeneration. Development. 2016;143:2066-76 pubmed publisher
Visscher D, Bos E, Peeters M, Kuzmin N, Groot M, Helder M, et al. Cartilage Tissue Engineering: Preventing Tissue Scaffold Contraction Using a 3D-Printed Polymeric Cage. Tissue Eng Part C Methods. 2016;22:573-84 pubmed publisher
Elsaesser A, Schwarz S, Joos H, Koerber L, Brenner R, Rotter N. Characterization of a migrative subpopulation of adult human nasoseptal chondrocytes with progenitor cell features and their potential for in vivo cartilage regeneration strategies. Cell Biosci. 2016;6:11 pubmed publisher
Tao K, Frisch J, Rey Rico A, Venkatesan J, Schmitt G, Madry H, et al. Co-overexpression of TGF-β and SOX9 via rAAV gene transfer modulates the metabolic and chondrogenic activities of human bone marrow-derived mesenchymal stem cells. Stem Cell Res Ther. 2016;7:20 pubmed publisher
Frisch J, Rey Rico A, Venkatesan J, Schmitt G, Madry H, Cucchiarini M. TGF-β gene transfer and overexpression via rAAV vectors stimulates chondrogenic events in human bone marrow aspirates. J Cell Mol Med. 2016;20:430-40 pubmed publisher
Bornes T, Jomha N, Mulet Sierra A, Adesida A. Optimal Seeding Densities for In Vitro Chondrogenesis of Two- and Three-Dimensional-Isolated and -Expanded Bone Marrow-Derived Mesenchymal Stromal Stem Cells Within a Porous Collagen Scaffold. Tissue Eng Part C Methods. 2016;22:208-20 pubmed publisher
Miura S, Takahashi Y, Satoh A, Endo T. Skeletal callus formation is a nerve-independent regenerative response to limb amputation in mice and Xenopus. Regeneration (Oxf). 2015;2:202-16 pubmed publisher
de Kroon L, Narcisi R, Blaney Davidson E, Cleary M, van Beuningen H, Koevoet W, et al. Activin Receptor-Like Kinase Receptors ALK5 and ALK1 Are Both Required for TGFβ-Induced Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells. PLoS ONE. 2015;10:e0146124 pubmed publisher
Enault S, Muñoz D, Silva W, Borday Birraux V, Bonade M, Oulion S, et al. Molecular footprinting of skeletal tissues in the catshark Scyliorhinus canicula and the clawed frog Xenopus tropicalis identifies conserved and derived features of vertebrate calcification. Front Genet. 2015;6:283 pubmed publisher
Johanson Z, Boisvert C, Maksimenko A, Currie P, Trinajstic K. Development of the Synarcual in the Elephant Sharks (Holocephali; Chondrichthyes): Implications for Vertebral Formation and Fusion. PLoS ONE. 2015;10:e0135138 pubmed publisher
Benders K, Boot W, Cokelaere S, van Weeren P, Gawlitta D, Bergman H, et al. Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds. Cartilage. 2014;5:221-30 pubmed publisher
Francisco Botelho J, Smith Paredes D, Soto Acuña S, Mpodozis J, Palma V, Vargas A. Skeletal plasticity in response to embryonic muscular activity underlies the development and evolution of the perching digit of birds. Sci Rep. 2015;5:9840 pubmed publisher
Burns F, Peterson R, Heideman W. Dioxin disrupts cranial cartilage and dermal bone development in zebrafish larvae. Aquat Toxicol. 2015;164:52-60 pubmed publisher
Bornes T, Jomha N, Mulet Sierra A, Adesida A. Hypoxic culture of bone marrow-derived mesenchymal stromal stem cells differentially enhances in vitro chondrogenesis within cell-seeded collagen and hyaluronic acid porous scaffolds. Stem Cell Res Ther. 2015;6:84 pubmed publisher
Zhang Y, Li J, Davis M, Pei M. Delineation of in vitro chondrogenesis of human synovial stem cells following preconditioning using decellularized matrix. Acta Biomater. 2015;20:39-50 pubmed publisher
Visser J, Gawlitta D, Benders K, Toma S, Pouran B, van Weeren P, et al. Endochondral bone formation in gelatin methacrylamide hydrogel with embedded cartilage-derived matrix particles. Biomaterials. 2015;37:174-82 pubmed publisher
Gawlitta D, Benders K, Visser J, van der Sar A, Kempen D, Theyse L, et al. Decellularized cartilage-derived matrix as substrate for endochondral bone regeneration. Tissue Eng Part A. 2015;21:694-703 pubmed publisher
Frisch J, Venkatesan J, Rey Rico A, Schmitt G, Madry H, Cucchiarini M. Influence of insulin-like growth factor I overexpression via recombinant adeno-associated vector gene transfer upon the biological activities and differentiation potential of human bone marrow-derived mesenchymal stem cells. Stem Cell Res Ther. 2014;5:103 pubmed publisher
Rosa R, Joazeiro P, Bianco J, Kunz M, Weber J, Waldman S. Growth factor stimulation improves the structure and properties of scaffold-free engineered auricular cartilage constructs. PLoS ONE. 2014;9:e105170 pubmed publisher
Jeon J, Vaquette C, Theodoropoulos C, Klein T, Hutmacher D. Multiphasic construct studied in an ectopic osteochondral defect model. J R Soc Interface. 2014;11:20140184 pubmed publisher
Boere K, Visser J, Seyednejad H, Rahimian S, Gawlitta D, van Steenbergen M, et al. Covalent attachment of a three-dimensionally printed thermoplast to a gelatin hydrogel for mechanically enhanced cartilage constructs. Acta Biomater. 2014;10:2602-11 pubmed publisher
Chon B, Lee E, Jing L, Setton L, Chen J. Human umbilical cord mesenchymal stromal cells exhibit immature nucleus pulposus cell phenotype in a laminin-rich pseudo-three-dimensional culture system. Stem Cell Res Ther. 2013;4:120 pubmed publisher
Prins H, Braat A, Gawlitta D, Dhert W, Egan D, Tijssen Slump E, et al. In vitro induction of alkaline phosphatase levels predicts in vivo bone forming capacity of human bone marrow stromal cells. Stem Cell Res. 2014;12:428-40 pubmed publisher
Croutze R, Jomha N, Uludag H, Adesida A. Matrix forming characteristics of inner and outer human meniscus cells on 3D collagen scaffolds under normal and low oxygen tensions. BMC Musculoskelet Disord. 2013;14:353 pubmed publisher
Kock L, Malda J, Dhert W, Ito K, Gawlitta D. Flow-perfusion interferes with chondrogenic and hypertrophic matrix production by mesenchymal stem cells. J Biomech. 2014;47:2122-9 pubmed publisher
Chen J, Lee E, Jing L, Christoforou N, Leong K, Setton L. Differentiation of mouse induced pluripotent stem cells (iPSCs) into nucleus pulposus-like cells in vitro. PLoS ONE. 2013;8:e75548 pubmed publisher
Markway B, Cho H, Johnstone B. Hypoxia promotes redifferentiation and suppresses markers of hypertrophy and degeneration in both healthy and osteoarthritic chondrocytes. Arthritis Res Ther. 2013;15:R92 pubmed publisher
Jurgens W, Kroeze R, Zandieh Doulabi B, van Dijk A, Renders G, Smit T, et al. One-step surgical procedure for the treatment of osteochondral defects with adipose-derived stem cells in a caprine knee defect: a pilot study. Biores Open Access. 2013;2:315-25 pubmed publisher
Chowdhury A, Bezuidenhout L, Mulet Sierra A, Jomha N, Adesida A. Effect of interleukin-1? treatment on co-cultures of human meniscus cells and bone marrow mesenchymal stromal cells. BMC Musculoskelet Disord. 2013;14:216 pubmed publisher
Bensimon Brito A, Cardeira J, Cancela M, Huysseune A, Witten P. Distinct patterns of notochord mineralization in zebrafish coincide with the localization of Osteocalcin isoform 1 during early vertebral centra formation. BMC Dev Biol. 2012;12:28 pubmed publisher
Schurgers L, Joosen I, Laufer E, Chatrou M, Herfs M, Winkens M, et al. Vitamin K-antagonists accelerate atherosclerotic calcification and induce a vulnerable plaque phenotype. PLoS ONE. 2012;7:e43229 pubmed publisher
Bries A, Weiner D, Jacquet R, Adamczyk M, Morscher M, Lowder E, et al. A study in vivo of the effects of a static compressive load on the proximal tibial physis in rabbits. J Bone Joint Surg Am. 2012;94:e1111-10 pubmed publisher
Saliken D, Mulet Sierra A, Jomha N, Adesida A. Decreased hypertrophic differentiation accompanies enhanced matrix formation in co-cultures of outer meniscus cells with bone marrow mesenchymal stromal cells. Arthritis Res Ther. 2012;14:R153 pubmed publisher
Adesida A, Mulet Sierra A, Laouar L, Jomha N. Oxygen tension is a determinant of the matrix-forming phenotype of cultured human meniscal fibrochondrocytes. PLoS ONE. 2012;7:e39339 pubmed publisher
Gawlitta D, Van Rijen M, Schrijver E, Alblas J, Dhert W. Hypoxia impedes hypertrophic chondrogenesis of human multipotent stromal cells. Tissue Eng Part A. 2012;18:1957-66 pubmed publisher
Matthies N, Mulet Sierra A, Jomha N, Adesida A. Matrix formation is enhanced in co-cultures of human meniscus cells with bone marrow stromal cells. J Tissue Eng Regen Med. 2013;7:965-73 pubmed publisher
Caron M, Emans P, Surtel D, Cremers A, Voncken J, Welting T, et al. Activation of NF-?B/p65 facilitates early chondrogenic differentiation during endochondral ossification. PLoS ONE. 2012;7:e33467 pubmed publisher
Adesida A, Mulet Sierra A, Jomha N. Hypoxia mediated isolation and expansion enhances the chondrogenic capacity of bone marrow mesenchymal stromal cells. Stem Cell Res Ther. 2012;3:9 pubmed publisher
Metscher B, Muller G. MicroCT for molecular imaging: quantitative visualization of complete three-dimensional distributions of gene products in embryonic limbs. Dev Dyn. 2011;240:2301-8 pubmed publisher
Knight R, Mebus K, D Angelo A, Yokoya K, Heanue T, Roehl H. Ret signalling integrates a craniofacial muscle module during development. Development. 2011;138:2015-24 pubmed publisher
van Buul G, Kotek G, Wielopolski P, Farrell E, Bos P, Weinans H, et al. Clinically translatable cell tracking and quantification by MRI in cartilage repair using superparamagnetic iron oxides. PLoS ONE. 2011;6:e17001 pubmed publisher
Farrell E, Both S, Odörfer K, Koevoet W, Kops N, O Brien F, et al. In-vivo generation of bone via endochondral ossification by in-vitro chondrogenic priming of adult human and rat mesenchymal stem cells. BMC Musculoskelet Disord. 2011;12:31 pubmed publisher
Kon E, Filardo G, Delcogliano M, Fini M, Salamanna F, Giavaresi G, et al. Platelet autologous growth factors decrease the osteochondral regeneration capability of a collagen-hydroxyapatite scaffold in a sheep model. BMC Musculoskelet Disord. 2010;11:220 pubmed publisher
Ströbel S, Loparic M, Wendt D, Schenk A, Candrian C, Lindberg R, et al. Anabolic and catabolic responses of human articular chondrocytes to varying oxygen percentages. Arthritis Res Ther. 2010;12:R34 pubmed publisher
Schuurman W, Gawlitta D, Klein T, ten Hoope W, Van Rijen M, Dhert W, et al. Zonal chondrocyte subpopulations reacquire zone-specific characteristics during in vitro redifferentiation. Am J Sports Med. 2009;37 Suppl 1:97S-104S pubmed publisher
He F, Chen X, Pei M. Reconstruction of an in vitro tissue-specific microenvironment to rejuvenate synovium-derived stem cells for cartilage tissue engineering. Tissue Eng Part A. 2009;15:3809-21 pubmed publisher
Huang C, Wang T, Lin B, Wang Y, Johnson S, Yu J. Collagen IX is required for the integrity of collagen II fibrils and the regulation of vascular plexus formation in zebrafish caudal fins. Dev Biol. 2009;332:360-70 pubmed publisher
Grogan S, Miyaki S, Asahara H, D Lima D, Lotz M. Mesenchymal progenitor cell markers in human articular cartilage: normal distribution and changes in osteoarthritis. Arthritis Res Ther. 2009;11:R85 pubmed publisher
Eshkar Oren I, Viukov S, Salameh S, Krief S, Oh C, Akiyama H, et al. The forming limb skeleton serves as a signaling center for limb vasculature patterning via regulation of Vegf. Development. 2009;136:1263-72 pubmed publisher
Suva M, Riggi N, Stehle J, Baumer K, Tercier S, Joseph J, et al. Identification of cancer stem cells in Ewing's sarcoma. Cancer Res. 2009;69:1776-81 pubmed publisher
Clément A, Wiweger M, von der Hardt S, Rusch M, Selleck S, Chien C, et al. Regulation of zebrafish skeletogenesis by ext2/dackel and papst1/pinscher. PLoS Genet. 2008;4:e1000136 pubmed publisher
Pei M, He F, Kish V, Vunjak Novakovic G. Engineering of functional cartilage tissue using stem cells from synovial lining: a preliminary study. Clin Orthop Relat Res. 2008;466:1880-9 pubmed publisher
Jurgens W, Oedayrajsingh Varma M, Helder M, Zandiehdoulabi B, Schouten T, Kuik D, et al. Effect of tissue-harvesting site on yield of stem cells derived from adipose tissue: implications for cell-based therapies. Cell Tissue Res. 2008;332:415-26 pubmed publisher
Shepard J, Krug H, LaFoon B, Hoffman S, Capehart A. Versican expression during synovial joint morphogenesis. Int J Biol Sci. 2007;3:380-4 pubmed
Stoop R, Albrecht D, Gaissmaier C, Fritz J, Felka T, Rudert M, et al. Comparison of marker gene expression in chondrocytes from patients receiving autologous chondrocyte transplantation versus osteoarthritis patients. Arthritis Res Ther. 2007;9:R60 pubmed
de Mos M, Koevoet W, Jahr H, Verstegen M, Heijboer M, Kops N, et al. Intrinsic differentiation potential of adolescent human tendon tissue: an in-vitro cell differentiation study. BMC Musculoskelet Disord. 2007;8:16 pubmed
Oedayrajsingh Varma M, van Ham S, Knippenberg M, Helder M, Klein Nulend J, Schouten T, et al. Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure. Cytotherapy. 2006;8:166-77 pubmed
Emans P, Hulsbosch M, Wetzels G, Bulstra S, Kuijer R. Repair of osteochondral defects in rabbits with ectopically produced cartilage. Tissue Eng. 2005;11:1789-96 pubmed
Emans P, Surtel D, Frings E, Bulstra S, Kuijer R. In vivo generation of cartilage from periosteum. Tissue Eng. 2005;11:369-77 pubmed
Bonadio J, Smiley E, Patil P, Goldstein S. Localized, direct plasmid gene delivery in vivo: prolonged therapy results in reproducible tissue regeneration. Nat Med. 1999;5:753-9 pubmed
Thalmann I. Collagen of accessory structures of organ of Corti. Connect Tissue Res. 1993;29:191-201 pubmed
Linsenmayer T, Hendrix M. Monoclonal antibodies to connective tissue macromolecules: type II collagen. Biochem Biophys Res Commun. 1980;92:440-6 pubmed
product information
Internal ID :
3015
Name :
II-II6B3
Depositor Name :
Linsenmayer, T.F.
Depositor Institution :
Tufts Medical School
Depositor Website :
http://sackler.tufts.edu/Faculty-and-Research/Faculty-Research-Pages/Thomas-Linsenmayer
Date Deposited :
3/3/93
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Collagen type II
Antigen Species :
Chicken
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Avian,Bovine,Broad species,Chicken,Fish,Goat,Human,Mouse,Ovine,Rabbit,Rat,Shark,Xenopus,Zebrafish
Species Tested (legacy) :
zebrafish, fish
Initial Publication Pubmed ID :
7356475
Collections :
Cell markers,Extracellular matrix proteins,Human,Stem cell,Xenopus
Search Keywords :
Linsenmayer, T.F., Collagen type II , Chicken, MIgG1, kappa light chain, Broad/Fish/Zebrafish/Ovine/Xenopus/Human/Chicken/Avian/Shark/Mouse/Goat, COL2A1, , RRID: AB_528165, Monoclonal, Human/Xenopus/Extracellular matrix proteins/Cell markers, Immunohistochemistry/Immunofluorescence/Western blot/FFPE
Antigen Molecular Weight :
141 kDa (predicted)
Gene :
COL2A1
Uniprot ID :
P02460
Antibody Registry ID :
AB_528165
Immunogen :
Pepsin-digest adult chick sternal cartilage
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
MPC 11
Epitope Mapped :
Yes
Epitope Location or Sequence :
Epitope lies within the triple-helical domain of chick collagen type II.
Epitope Map PubMed ID :
7356475
Recommended Applications :
FFPE,Immunofluorescence,Immunohistochemistry,Western Blot
Immunohistochemistry Pubmed IDs :
23043290 21490065 19501583 18654627 25900045 22473741 24330551 22726892 22720095 22385573 23043290 21490065 18654627 7356475 27432980 27340110 26651081 26720610 31363834 22855000 27128439 27431733 26339918 27499875 23914338 25316202 25453948 24384458 24290176 26069701 24590160 22563686 28229956 27171342 19846691 27089896 30572166 30733134 31369767 31402442
Immunofluorescence Pubmed IDs :
27292641 19501583 7356475 26442101 26339918 25974685
Western Blot Pubmed IDs :
19545204 8222646 18512111
FFPE Pubmed IDs :
21281488 16698690 10395319 19208848 31369767
Pubmed IDs :
23043290 21490065 19501583 18654627
DSHB Growth Medium :
Iscove's
References (legacy) :
Biochem. Biophys. Res. Comm. 92, 440-446.; Dev. Dyn. 200, 294-304.; Exp. Cell Res. 248, 294-305.; Clin. Imp. Dent. Rel. Res. 1(1), 33-40.; J. Biomed. Mater. Res. 51, 280-291.; Biochem. Biophys. Res. Commun. 273, 609-613.; Tissue Eng. 9(5), 939-948.; Osteoarthritis Cartilage 12, 306-313.; Tissue Eng. 11(3-4), 369-377.; Tissue Eng. 11(11-12), 1789-1796.; Bone 40, 409-418.; Development 136, 1263-1272.; Dev. Dyn. 240, 2301-2308.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US