product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
HSP-60
catalog :
HSP60
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
HSP60
reactivity :
Caenorhabditis elegans
application :
western blot, immunohistochemistry, immunocytochemistry
citations: 14
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; Caenorhabditis elegans; 1:10; tbl 2
Smurova K, Podbilewicz B. RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion. Cell Rep. 2016;14:1517-1527 pubmed publisher
Rolland S, Schneid S, Schwarz M, Rackles E, Fischer C, Haeussler S, et al. Compromised Mitochondrial Protein Import Acts as a Signal for UPRmt. Cell Rep. 2019;28:1659-1669.e5 pubmed publisher
Tulli S, Del Bondio A, Baderna V, Mazza D, Codazzi F, Pierson T, et al. Pathogenic variants in the AFG3L2 proteolytic domain cause SCA28 through haploinsufficiency and proteostatic stress-driven OMA1 activation. J Med Genet. 2019;56:499-511 pubmed publisher
Kamath A, Deehan M, Frydman H. Polar cell fate stimulates Wolbachia intracellular growth. Development. 2018;145: pubmed publisher
Elenbaas J, Bragazzi Cunha J, Azuero Dajud R, Nelson B, Oral E, Williams J, et al. Lamin A/C Maintains Exocrine Pancreas Homeostasis by Regulating Stability of RB and Activity of E2F. Gastroenterology. 2018;154:1625-1629.e8 pubmed publisher
Chen X, Wang Y, Chen Y, Harry B, Nakagawa A, Lee E, et al. Regulation of CED-3 caspase localization and activation by C. elegans nuclear-membrane protein NPP-14. Nat Struct Mol Biol. 2016;23:958-964 pubmed publisher
Kim H, Grant A, Simic M, Kohnz R, Nomura D, Durieux J, et al. Lipid Biosynthesis Coordinates a Mitochondrial-to-Cytosolic Stress Response. Cell. 2016;166:1539-1552.e16 pubmed publisher
Trapannone R, Mariappa D, Ferenbach A, van Aalten D. Nucleocytoplasmic human O-GlcNAc transferase is sufficient for O-GlcNAcylation of mitochondrial proteins. Biochem J. 2016;473:1693-702 pubmed publisher
Barone R, Macaluso F, Sangiorgi C, Campanella C, Marino Gammazza A, Moresi V, et al. Skeletal muscle Heat shock protein 60 increases after endurance training and induces peroxisome proliferator-activated receptor gamma coactivator 1 α1 expression. Sci Rep. 2016;6:19781 pubmed publisher
Gamerdinger M, Hanebuth M, Frickey T, Deuerling E. The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum. Science. 2015;348:201-7 pubmed publisher
Grün D, Kirchner M, Thierfelder N, Stoeckius M, Selbach M, Rajewsky N. Conservation of mRNA and protein expression during development of C. elegans. Cell Rep. 2014;6:565-77 pubmed publisher
Mitra K, Rikhy R, Lilly M, Lippincott Schwartz J. DRP1-dependent mitochondrial fission initiates follicle cell differentiation during Drosophila oogenesis. J Cell Biol. 2012;197:487-97 pubmed publisher
Cheng L, Li B, Wang Z. Level-Set Minimization of Potential Controlled Hadwiger Valuations for Molecular Solvation. J Comput Phys. 2010;229:8497-8510 pubmed
Hadwiger G, Dour S, Arur S, Fox P, Nonet M. A monoclonal antibody toolkit for C. elegans. PLoS ONE. 2010;5:e10161 pubmed publisher
product information
Internal ID :
6488
Name :
HSP60
Depositor Name :
Nonet, M.L. / Hadwiger, G. / Dour, S.
Depositor Institution :
Washington University Medical School
Date Deposited :
2/16/10
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
HSP-60
Antigen Species :
C. elegans
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
C. briggsae,C. elegans
Species Tested (legacy) :
C. elegans
Initial Publication Pubmed ID :
20405020
Depositor Notes (Special Instructions) :
Works on C. elegans whole mounts
Collections :
C. elegans
Search Keywords :
Nonet, M.L. / Hadwiger, G. / Dour, S., HSP-60, C. elegans, MIgG1, C. elegans/C. briggsae, hsp-60, , AB_10572252, monoclonal, C. elegans, Immunofluorescence/Western Blot
Antigen Molecular Weight :
Predicted: 60 kDa; Apparent: 65 kDa
Gene :
hsp-60
Uniprot ID :
P50140
Antibody Registry ID :
AB_10572252
Synonyms (Alternate Clone Names) :
1H12
Immunogen :
Recombinant His6-tagged HSP-60 (aa 1-547) fusion protein
Immunogen Sequence :
Partial protein
Clonality :
Monoclonal
Myeloma Strain :
P3X63Ag8.653
Epitope Mapped :
No
Epitope Map PubMed ID :
20405020
Recommended Applications :
Immunofluorescence,Western Blot
Immunoblotting (legacy) :
good
Immunofluorescence Pubmed IDs :
20405020 26854231
Western Blot Pubmed IDs :
20405020 27723735 25859040 24462290 27610574
Additional Information :
The Chaperonin homolog Hsp-60, mitochondrial, is a mitochondrial targeted heat shock protein useful as a marker of mitochondria
DSHB Growth Medium :
Iscove's
References (legacy) :
Hadwiger, G., Dour, S., Arur, S., Fox, P., and Nonet, M.L. (2010) A monoclonal antibody toolkit for C. elegans. PLoS One. 5(4):e10161-10115.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US