Otoferlin; Hair cell soma-1 | Developmental Studies Hybridoma Bank HCS-1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Otoferlin; Hair cell soma-1

catalog :

HCS-1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HCS-1

reactivity :

human, chicken, zebrafish

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; zebrafish ; 1:750; loading ...; fig 1a	Manchanda A, Bonventre J, Bugel S, Chatterjee P, Tanguay R, Johnson C. Truncation of the otoferlin transmembrane domain alters the development of hair cells and reduces membrane docking. Mol Biol Cell. 2021;32:1293-1305 pubmed publisher
immunohistochemistry; chicken; 1:250; loading ...; fig 3d	Zhu Y, Scheibinger M, Ellwanger D, Krey J, Choi D, Kelly R, et al. Single-cell proteomics reveals changes in expression during hair-cell development. elife. 2019;8: pubmed publisher
	Fogerty J, Stepanyan R, Cianciolo L, Tooke B, Perkins B. Genomic non-redundancy of the mir-183/96/182 cluster and its requirement for hair cell maintenance. Sci Rep. 2019;9:10302 pubmed publisher
	Munnamalai V, Sienknecht U, Duncan R, Scott M, Thawani A, Fantetti K, et al. Wnt9a Can Influence Cell Fates and Neural Connectivity across the Radial Axis of the Developing Cochlea. J Neurosci. 2017;37:8975-8988 pubmed publisher
	Pei W, Xu L, Varshney G, Carrington B, Bishop K, Jones M, et al. Additive reductions in zebrafish PRPS1 activity result in a spectrum of deficiencies modeling several human PRPS1-associated diseases. Sci Rep. 2016;6:29946 pubmed publisher
	Agarwala S, Duquesne S, Liu K, Boehm A, Grimm L, Link S, et al. Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafish. elife. 2015;4:e08201 pubmed publisher
	Zhang K, Stoller M, Fekete D. Expression and Misexpression of the miR-183 Family in the Developing Hearing Organ of the Chicken. PLoS ONE. 2015;10:e0132796 pubmed publisher
	He Y, Cai C, Tang D, Sun S, Li H. Effect of histone deacetylase inhibitors trichostatin A and valproic acid on hair cell regeneration in zebrafish lateral line neuromasts. Front Cell Neurosci. 2014;8:382 pubmed publisher
	Ogun O, Zallocchi M. Clarin-1 acts as a modulator of mechanotransduction activity and presynaptic ribbon assembly. J Cell Biol. 2014;207:375-91 pubmed publisher
	Goodyear R, Legan P, Christiansen J, Xia B, Korchagina J, Gale J, et al. Identification of the hair cell soma-1 antigen, HCS-1, as otoferlin. J Assoc Res Otolaryngol. 2010;11:573-86 pubmed publisher

product information

Internal ID :

6840

Name :

HCS-1

Depositor Name :

Corwin, J.

Depositor Institution :

University of Virginia

Depositor Website :

https://med.virginia.edu/neuroscience/sensory-neuroscience/corwin-lab/

Date Deposited :

11/16/10

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Otoferlin; Hair cell soma-1

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgG2a

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Amphibian,Avian,Elasmobranch,Fish,Mammal,Reptile

Species Tested (legacy) :

elasmobranchs, bony fishes, amphibians, mammals, birds and reptiles

Initial Publication Pubmed ID :

20809368

Depositor Notes (Special Instructions) :

This antigen is a key calcium ion sensor regulating neurotransmitter release. The antigen is also known as Fer-1-like protein 2.

Collections :

Cell markers,Human,Neurodevelopment,Xenopus

Search Keywords :

Corwin, Jeffrey T. , Otoferlin; Hair cell soma-1, Chicken, MIgG2a, Elasmobranch Fish Amphibian Mammal Avian Reptile, OTOF, DFNB6, DFNB9, FER1L2, AB_10804296, Monoclonal , Human zebrafish xenopus Neurodevelopment, Immunohistochemistry Immunofluorescence Immunoprecipitation

Antigen Molecular Weight :

210 kDa /230 kDa

Gene :

OTOF

Alternate Gene Name(s) :

DFNB6, DFNB9, FER1L2

Uniprot ID :

Q9HC10

Antibody Registry ID :

AB_10804296

Synonyms (Alternate Clone Names) :

otoferlin

Immunogen :

Delaminated chick utricles emulsion

Immunogen Sequence :

Full length sequence

Clonality :

Monoclonal

Myeloma Strain :

SP/0

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation

Immunoblotting (legacy) :

Immunohistochemistry Pubmed IDs :

26335201 28821654

Immunofluorescence Pubmed IDs :

20809368 26176784 31311951

Immunoprecipitation Pubmed IDs :

20809368

Additional Information :

RRID:AB_10804296

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Assoc. Res. Otolaryngol. 11(4), 573-586.

more info or order :

Developmental Studies Hybridoma Bank product webpage