product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
sucrase-isomaltase, sucrase subunit
catalog :
HBB2/614/88
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
HBB2/614/88
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 12
Reference
Gericke B, Schecker N, Amiri M, Naim H. Structure-function analysis of human sucrase-isomaltase identifies key residues required for catalytic activity. J Biol Chem. 2017;292:11070-11078 pubmed publisher
Gericke B, Amiri M, Scott C, Naim H. Molecular pathogenicity of novel sucrase-isomaltase mutations found in congenital sucrase-isomaltase deficiency patients. Biochim Biophys Acta Mol Basis Dis. 2017;1863:817-826 pubmed publisher
Amiri M, Kuech E, Shammas H, Wetzel G, Naim H. The Pathobiochemistry of Gastrointestinal Symptoms in a Patient with Niemann-Pick Type C Disease. JIMD Rep. 2016;25:25-29 pubmed
Vogel G, Klee K, Janecke A, Müller T, Hess M, Huber L. Cargo-selective apical exocytosis in epithelial cells is conducted by Myo5B, Slp4a, Vamp7, and Syntaxin 3. J Cell Biol. 2015;211:587-604 pubmed publisher
Thompson Fawcett M, McC Mortensen N, Jewel D, Warren B. Use of a monoclonal antibody to sucrase-isomaltase for evaluation of the columnar cuff after stapled restorative proctocolectomy. Dis Colon Rectum. 2007;50:1428-35 pubmed
Hauri H, Roth J, Sterchi E, Lentze M. Transport to cell surface of intestinal sucrase-isomaltase is blocked in the Golgi apparatus in a patient with congenital sucrase-isomaltase deficiency. Proc Natl Acad Sci U S A. 1985;82:4423-7 pubmed
Hauri H, Sterchi E, Bienz D, Fransen J, Marxer A. Expression and intracellular transport of microvillus membrane hydrolases in human intestinal epithelial cells. J Cell Biol. 1985;101:838-51 pubmed
Stieger B, Matter K, Baur B, Bucher K, Höchli M, Hauri H. Dissection of the asynchronous transport of intestinal microvillar hydrolases to the cell surface. J Cell Biol. 1988;106:1853-61 pubmed
Czernichow B, Simon Assmann P, Kedinger M, Arnold C, Parache M, Marescaux J, et al. Sucrase-isomaltase expression and enterocytic ultrastructure of human colorectal tumors. Int J Cancer. 1989;44:238-44 pubmed
Hauri H, Bucher K. Immunoblotting with monoclonal antibodies: importance of the blocking solution. Anal Biochem. 1986;159:386-9 pubmed
Bagli D, D Emilia J, Summerhayes I, Steele G, Barlozzari T. c-Ha-ras-I oncogene-induced differentiation and natural killer cell resistance in a human colorectal carcinoma cell line. Cancer Res. 1990;50:2518-23 pubmed
de Silva H, Millard P, Kettlewell M, Mortensen N, Prince C, Jewell D. Mucosal characteristics of pelvic ileal pouches. Gut. 1991;32:61-5 pubmed
product information
Internal ID :
6376
Name :
HBB2/614/88
Depositor Name :
Hauri, H.-P.
Depositor Institution :
Biozentrum, University of Basel, Switzerland
Date Deposited :
2/8/10
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
sucrase-isomaltase, sucrase subunit
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Human
Species Tested (legacy) :
human
Initial Publication Pubmed ID :
3897250
Collections :
Enzymes,Human
Search Keywords :
Hauri, H.-P., sucrase-isomaltase, sucrase subunit, Human, MIgG1, Human, SI, , AB_10571463, monoclonal, Enzymes/Human, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/ELISA/FFPE
Antigen Molecular Weight :
Predicted: 209 kDa (pro-SI), 95 kDa (Sucrase), 114 kDa (Isomaltase); Apparent: 231 kDa (pro-SI), 145 kDa (S), 151 kDa (I)
Gene :
SI
Uniprot ID :
P14410
Antibody Registry ID :
AB_10571463
Immunogen :
purified intestinal brush border membrane from normal adult human jejunal mucosa
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
F0
Epitope Mapped :
Yes
Epitope Location or Sequence :
Sucrase subunit (aa 1006-1825)
Epitope Map PubMed ID :
3897250
Recommended Applications :
ELISA,FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
1846839 17665257
Immunofluorescence Pubmed IDs :
3897250 2898478 2759730 26553929 2180571
Western Blot Pubmed IDs :
3897250 3925457 28062276 26122626 2435189
Immunoprecipitation Pubmed IDs :
3897250 2898478 28062276
ELISA Pubmed IDs :
3897250
FFPE Pubmed IDs :
17665257
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Cell Biol. 101, 838-851.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US