product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
sucrase-isomaltase
catalog :
HBB2/219/20
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
HBB2/219/20
reactivity :
human
application :
ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 8
Reference
Gericke B, Amiri M, Scott C, Naim H. Molecular pathogenicity of novel sucrase-isomaltase mutations found in congenital sucrase-isomaltase deficiency patients. Biochim Biophys Acta Mol Basis Dis. 2017;1863:817-826 pubmed publisher
Faller G, Dimmler A, Rau T, Spaderna S, Hlubek F, Jung A, et al. Evidence for acid-induced loss of Cdx2 expression in duodenal gastric metaplasia. J Pathol. 2004;203:904-8 pubmed
Van De Bovenkamp J, Korteland Van Male A, Büller H, Einerhand A, Dekker J. Metaplasia of the duodenum shows a Helicobacter pylori-correlated differentiation into gastric-type protein expression. Hum Pathol. 2003;34:156-65 pubmed
van Beers E, Al R, Rings E, Einerhand A, Dekker J, Buller H. Lactase and sucrase-isomaltase gene expression during Caco-2 cell differentiation. Biochem J. 1995;308 ( Pt 3):769-75 pubmed
Naim H. Human small intestinal angiotensin-converting enzyme: intracellular transport, secretion and glycosylation. Biochem J. 1993;296 ( Pt 3):607-15 pubmed
Hauri H, Sterchi E, Bienz D, Fransen J, Marxer A. Expression and intracellular transport of microvillus membrane hydrolases in human intestinal epithelial cells. J Cell Biol. 1985;101:838-51 pubmed
Stieger B, Matter K, Baur B, Bucher K, Höchli M, Hauri H. Dissection of the asynchronous transport of intestinal microvillar hydrolases to the cell surface. J Cell Biol. 1988;106:1853-61 pubmed
Hauri H, Bucher K. Immunoblotting with monoclonal antibodies: importance of the blocking solution. Anal Biochem. 1986;159:386-9 pubmed
product information
Internal ID :
6381
Name :
HBB2/219/20
Depositor Name :
Hauri, H.-P.
Depositor Institution :
Biozentrum, University of Basel, Switzerland
Date Deposited :
2/8/10
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
sucrase-isomaltase
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG2b
Isotype for catalog (legacy) :
IgG2b
Positive Tested Species Reactivity :
Human
Species Tested (legacy) :
human
Initial Publication Pubmed ID :
3897250
Collections :
Enzymes,Human
Search Keywords :
Hauri, H.-P., sucrase-isomaltase, Human, MIgG2b, Human, SI, , AB_2188726, monoclonal, Enzymes/Human, Immunohistochemistry/Immunofluorescence/Immunoprecipitation/ELISA
Antigen Molecular Weight :
Predicted: 209 kDa (pro-SI), 95 kDa (Sucrase), 114 kDa (Isomaltase); Apparent: 231 kDa (pro-SI), 145 kDa (S), 151 kDa (I)
Gene :
SI
Uniprot ID :
P14410
Antibody Registry ID :
AB_2188726
Immunogen :
purified intestinal brush border membrane from normal adult human jejunal mucosa
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
F0
Epitope Mapped :
No
Recommended Applications :
ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation
Immunoblotting (legacy) :
no
Immunohistochemistry Pubmed IDs :
8948431 15258992 12612884
Immunofluorescence Pubmed IDs :
3897250
Immunoprecipitation Pubmed IDs :
3897250 2898478 28062276 8948431 2435189 8280058
ELISA Pubmed IDs :
3897250
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Cell Biol. 101, 838-851.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US