CD63 (human) LAMP-3 | Developmental Studies Hybridoma Bank H5C6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

CD63 (human) LAMP-3

catalog :

H5C6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

H5C6

reactivity :

human, mouse, rat, dog, pig

application :

ELISA, immunocytochemistry, immunoprecipitation, flow cytometry, FACS, other

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 30

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 7b	Son K, Mukherjee M, McIntyre B, Eguez J, Radford K, LaVigne N, et al. Improved recovery of functionally active eosinophils and neutrophils using novel immunomagnetic technology. J Immunol Methods. 2017;449:44-55 pubmed publisher
immunocytochemistry; mouse; loading ...; fig ev2a	Wilhelm L, Wendling C, Vedie B, Kobayashi T, Chenard M, Tomasetto C, et al. STARD3 mediates endoplasmic reticulum-to-endosome cholesterol transport at membrane contact sites. EMBO J. 2017;36:1412-1433 pubmed publisher
immunocytochemistry; human; loading ...; fig 3a	Stephen J, Yokoyama T, Tolman N, O Brien K, Nicoli E, Brooks B, et al. Cellular and molecular defects in a patient with Hermansky-Pudlak syndrome type 5. PLoS ONE. 2017;12:e0173682 pubmed publisher
other; human; loading ...; fig 3	Su W, Kowalczyk A. The VE-cadherin cytoplasmic domain undergoes proteolytic processing during endocytosis. Mol Biol Cell. 2017;28:76-84 pubmed publisher
immunocytochemistry; rat; fig 1c	López Montero N, Ramos Marquès E, Risco C, Garcia del Portillo F. Intracellular Salmonella induces aggrephagy of host endomembranes in persistent infections. Autophagy. 2016;12:1886-1901 pubmed
flow cytometry; human; fig 1	Ventress J, Partridge L, Read R, Cozens D, MacNeil S, Monk P. Peptides from Tetraspanin CD9 Are Potent Inhibitors of Staphylococcus Aureus Adherence to Keratinocytes. PLoS ONE. 2016;11:e0160387 pubmed publisher
immunocytochemistry; pig; 1:25; loading ...; fig 4a	Hernaez B, Guerra M, Salas M, Andres G. African Swine Fever Virus Undergoes Outer Envelope Disruption, Capsid Disassembly and Inner Envelope Fusion before Core Release from Multivesicular Endosomes. PLoS Pathog. 2016;12:e1005595 pubmed publisher
immunocytochemistry; dog; 1:100; fig 3	Carrasco RamÃrez P, Greening D, AndrÃ©s G, Gopal S, MartÃn Villar E, Renart J, et al. Podoplanin is a component of extracellular vesicles that reprograms cell-derived exosomal proteins and modulates lymphatic vessel formation. Oncotarget. 2016;7:16070-89 pubmed publisher
immunocytochemistry; human; fig s4	Rebsamen M, Pochini L, Stasyk T, de AraÃºjo M, Galluccio M, Kandasamy R, et al. SLC38A9 is a component of the lysosomal amino acid sensing machinery that controls mTORC1. Nature. 2015;519:477-81 pubmed publisher
	Sadovska L, Zandberga E, Sagini K, JÄ“kabsons K, RiekstiÅ†a U, KalniÅ†a Z, et al. A novel 3D heterotypic spheroid model for studying extracellular vesicle-mediated tumour and immune cell communication. Biochem Biophys Res Commun. 2018;495:1930-1935 pubmed publisher
	Royo F, Zuñiga García P, Torrano V, Loizaga A, Sánchez Mosquera P, Ugalde Olano A, et al. Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer. Oncotarget. 2016;7:6835-46 pubmed publisher
	Shtanko O, Nikitina R, Altuntas C, Chepurnov A, Davey R. Crimean-Congo hemorrhagic fever virus entry into host cells occurs through the multivesicular body and requires ESCRT regulators. PLoS Pathog. 2014;10:e1004390 pubmed publisher
	Hessvik N, Phuyal S, Brech A, Sandvig K, Llorente A. Profiling of microRNAs in exosomes released from PC-3 prostate cancer cells. Biochim Biophys Acta. 2012;1819:1154-63 pubmed publisher
	Bastos Amador P, Royo F, Gonzalez E, Conde Vancells J, Palomo Diez L, Borras F, et al. Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability. J Proteomics. 2012;75:3574-84 pubmed publisher
	Lässer C, O Neil S, Ekerljung L, Ekström K, Sjöstrand M, Lotvall J. RNA-containing exosomes in human nasal secretions. Am J Rhinol Allergy. 2011;25:89-93 pubmed publisher
	Flannery A, Czibener C, Andrews N. Palmitoylation-dependent association with CD63 targets the Ca2+ sensor synaptotagmin VII to lysosomes. J Cell Biol. 2010;191:599-613 pubmed publisher
	Krementsov D, Weng J, Lambelé M, Roy N, Thali M. Tetraspanins regulate cell-to-cell transmission of HIV-1. Retrovirology. 2009;6:64 pubmed publisher
	Parthasarathy V, Martin F, Higginbottom A, Murray H, Moseley G, Read R, et al. Distinct roles for tetraspanins CD9, CD63 and CD81 in the formation of multinucleated giant cells. Immunology. 2009;127:237-48 pubmed publisher
	Logozzi M, De Milito A, Lugini L, Borghi M, Calabro L, Spada M, et al. High levels of exosomes expressing CD63 and caveolin-1 in plasma of melanoma patients. PLoS ONE. 2009;4:e5219 pubmed publisher
	Bampton E, Goemans C, Niranjan D, Mizushima N, Tolkovsky A. The dynamics of autophagy visualized in live cells: from autophagosome formation to fusion with endo/lysosomes. Autophagy. 2005;1:23-36 pubmed
	Nydegger S, Khurana S, Krementsov D, Foti M, Thali M. Mapping of tetraspanin-enriched microdomains that can function as gateways for HIV-1. J Cell Biol. 2006;173:795-807 pubmed
	Huber V, Fais S, Iero M, Lugini L, Canese P, Squarcina P, et al. Human colorectal cancer cells induce T-cell death through release of proapoptotic microvesicles: role in immune escape. Gastroenterology. 2005;128:1796-804 pubmed
	Duffield A, Kamsteeg E, Brown A, Pagel P, Caplan M. The tetraspanin CD63 enhances the internalization of the H,K-ATPase beta-subunit. Proc Natl Acad Sci U S A. 2003;100:15560-5 pubmed
	Ackerman A, Kyritsis C, Tampe R, Cresswell P. Early phagosomes in dendritic cells form a cellular compartment sufficient for cross presentation of exogenous antigens. Proc Natl Acad Sci U S A. 2003;100:12889-94 pubmed
	Arunachalam B, Phan U, Geuze H, Cresswell P. Enzymatic reduction of disulfide bonds in lysosomes: characterization of a gamma-interferon-inducible lysosomal thiol reductase (GILT). Proc Natl Acad Sci U S A. 2000;97:745-50 pubmed
	Serru V, Le Naour F, Billard M, Azorsa D, Lanza F, Boucheix C, et al. Selective tetraspan-integrin complexes (CD81/alpha4beta1, CD151/alpha3beta1, CD151/alpha6beta1) under conditions disrupting tetraspan interactions. Biochem J. 1999;340 ( Pt 1):103-11 pubmed
	Scherberich A, Moog S, Haan Archipoff G, Azorsa D, Lanza F, Beretz A. Tetraspanin CD9 is associated with very late-acting integrins in human vascular smooth muscle cells and modulates collagen matrix reorganization. Arterioscler Thromb Vasc Biol. 1998;18:1691-7 pubmed
	Hammond C, Denzin L, Pan M, Griffith J, Geuze H, Cresswell P. The tetraspan protein CD82 is a resident of MHC class II compartments where it associates with HLA-DR, -DM, and -DO molecules. J Immunol. 1998;161:3282-91 pubmed
	Azorsa D, Hyman J, Hildreth J. CD63/Pltgp40: a platelet activation antigen identical to the stage-specific, melanoma-associated antigen ME491. Blood. 1991;78:280-4 pubmed
	Hildreth J, Derr D, Azorsa D. Characterization of a novel self-associating Mr 40,000 platelet glycoprotein. Blood. 1991;77:121-32 pubmed

product information

Internal ID :

537

Name :

H5C6

Depositor Name :

August, J.T. / Hildreth, J.E.K.

Depositor Institution :

The Johns Hopkins University School of Medicine

Date Deposited :

4/12/93

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

CD63 (human) LAMP-3

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human,Porcine

Species Tested (legacy) :

human

Initial Publication Pubmed ID :

1984792

Collections :

CD antigens,Cell signaling,Human,Neurodevelopment

Search Keywords :

August, J.T. / Hildreth, J.E.K., CD63 (human) LAMP, Human, MIgG1, kappa light chain, Human/Porcine, CD63, MLA1, TSPAN30, ME491, LAMP-3, OMA81H, AB_528158, monoclonal, Cluster Determinant antigens/Human/Cell signaling, Immunofluorescence/Immunoprecipitation/ELISA/Function Blocking/FACS

Antigen Molecular Weight :

Predicted: 26 kDa; Apparent: heterodisperse glycoprotein of usually 40 to 55 kDa

Gene :

CD63

Alternate Gene Name(s) :

MLA1, TSPAN30, ME491, LAMP-3, OMA81H

Uniprot ID :

P08962

Antibody Registry ID :

AB_528158

Synonyms (Alternate Clone Names) :

anti-Pltg~4

Immunogen :

Human adherent spleen cells

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3X653-Ag8

Epitope Mapped :

Yes

Epitope Location or Sequence :

Recognizes an extracellular epitope: extracellular domain 1 or 2, corresponding to aa 33-51 and 103-203, respectively.

Recommended Applications :

ELISA,FACS,Function Blocking,Immunofluorescence,Immunoprecipitation

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

16874023 25233119 14561893 10639150 9759843 31314175

Western Blot Pubmed IDs :

10229664 19381331 14660791 29248729 15940614 22516433 22982408 21041449 9759843

Immunoprecipitation Pubmed IDs :

1984792 2070066 10229664 9759843

ELISA Pubmed IDs :

19381331

Function Blocking Pubmed IDs :

19489128

FACS Pubmed IDs :

1984792 10229664 19381331 15940614 9812906 22516433 21172122

Pubmed IDs :

25233119

Additional Information :

RRID:AB_528158

DSHB Growth Medium :

Iscove's

References (legacy) :

Anat. Rec. Part A 276A, 75-102.; Autophagy, 1(1), 23-36.; PLoS Pathog. 10(9): e1004390. doi:10.1371/journal.ppat.1004390.

more info or order :

Developmental Studies Hybridoma Bank product webpage