CD11b (Integrin alpha-M, Mac-1 alpha chain), human | Developmental Studies Hybridoma Bank H5A4 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

CD11b (Integrin alpha-M, Mac-1 alpha chain), human

catalog :

H5A4

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

H5A4

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 10

Reference
Guirado E, Mbawuike U, Keiser T, Arcos J, Azad A, Wang S, et al. Characterization of host and microbial determinants in individuals with latent tuberculosis infection using a human granuloma model. MBio. 2015;6:e02537-14 pubmed publisher
Ueno N, Bratt C, Rodriguez N, Wilson M. Differences in human macrophage receptor usage, lysosomal fusion kinetics and survival between logarithmic and metacyclic Leishmania infantum chagasi promastigotes. Cell Microbiol. 2009;11:1827-41 pubmed publisher
Edwards J, Apicella M. Neisseria gonorrhoeae PLD directly interacts with Akt kinase upon infection of primary, human, cervical epithelial cells. Cell Microbiol. 2006;8:1253-71 pubmed
Young H, Duplaa C, Yost M, Henson N, Floyd J, Detmer K, et al. Clonogenic analysis reveals reserve stem cells in postnatal mammals. II. Pluripotent epiblastic-like stem cells. Anat Rec A Discov Mol Cell Evol Biol. 2004;277:178-203 pubmed
Young H, Black A. Adult stem cells. Anat Rec A Discov Mol Cell Evol Biol. 2004;276:75-102 pubmed
Edwards J, Entz D, Apicella M. Gonococcal phospholipase d modulates the expression and function of complement receptor 3 in primary cervical epithelial cells. Infect Immun. 2003;71:6381-91 pubmed
Edwards J, Apicella M. The role of lipooligosaccharide in Neisseria gonorrhoeae pathogenesis of cervical epithelia: lipid A serves as a C3 acceptor molecule. Cell Microbiol. 2002;4:585-98 pubmed
Edwards J, Brown E, Ault K, Apicella M. The role of complement receptor 3 (CR3) in Neisseria gonorrhoeae infection of human cervical epithelia. Cell Microbiol. 2001;3:611-22 pubmed
Taylor M, Brummet M, Hudson S, Miura K, Bochner B. Expression and function of P-selectin glycoprotein ligand 1 (CD162) on human basophils. J Allergy Clin Immunol. 2000;106:918-24 pubmed
Hildreth J, August J. The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): functional effects of subunit-specific monoclonal antibodies. J Immunol. 1985;134:3272-80 pubmed

product information

Internal ID :

519

Name :

H5A4

Depositor Name :

August, J.T. / Hildreth, J.E.K.

Depositor Institution :

The Johns Hopkins University School of Medicine

Date Deposited :

4/12/93

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

CD11b (Integrin alpha-M, Mac-1 alpha chain), human

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human

Species Tested (legacy) :

human

Initial Publication Pubmed ID :

2580022

Depositor Notes (Special Instructions) :

Does not work on pig. Blocks leukocyte adhesion and inhibits T cell activity; no effect on NK cell activity. Stains frozen sections of human tonsil. Binds to PBMC.

Collections :

CD antigens,Cell adhesion,Cell markers,Cell signaling,Extracellular matrix proteins,Human,Stem cell

Search Keywords :

August, J.T. / Hildreth, J.E.K., CD11b (Integrin alpha-M, Mac-1 alpha chain), human, Human, MIgG1, Human, ITGAM, MAC-1, MO1A, CR3, AB_528134, monoclonal, Human/Stem cell/Cluster Determinant antigens/Cell signaling/Cell adhesion/Cell markers/Extracellular matrix proteins, Immunohistochemistry/Immunofluorescence/Immunoprecipitation/Function Blocking/ELISA/Western Blot/FACS

Antigen Molecular Weight :

Predicted: 127 kDa; Apparent: 170 kDa

Gene :

ITGAM

Alternate Gene Name(s) :

MAC-1, MO1A, CR3

Uniprot ID :

P11215

Antibody Registry ID :

AB_528134

Immunogen :

Adult Human adherent spleen cells

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3X653-Ag8

Epitope Mapped :

Recommended Applications :

ELISA,FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunohistochemistry Pubmed IDs :

2580022 11553013

Immunofluorescence Pubmed IDs :

25691598 19702651 11553013 12390351

Western Blot Pubmed IDs :

11553013

Immunoprecipitation Pubmed IDs :

2580022 11553013 12390351

ELISA Pubmed IDs :

16882030 14573659

Function Blocking Pubmed IDs :

2580022 16882030 11553013 14573659

FACS Pubmed IDs :

11080715

Pubmed IDs :

14699636 14983513

Additional Information :

Mac-1 is a macrophage differenciation antigen and a marker for microglia.

DSHB Growth Medium :

Iscove's

References (legacy) :

Anat. Rec. Part A 276A, 75-102.

more info or order :

Developmental Studies Hybridoma Bank product webpage