CD18 (integrin beta-2; beta subunit of CD11a, b, c), human | Developmental Studies Hybridoma Bank H52 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

CD18 (integrin beta-2; beta subunit of CD11a, b, c), human

catalog :

H52

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

H52

reactivity :

human, dog

application :

western blot, immunohistochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Reference
Guedon J, Luo K, Zhang H, Markham R. Monoclonal and Single Domain Antibodies Targeting Î²-Integrin Subunits Block Sexual Transmission of HIV-1 in In Vitro and In Vivo Model Systems. J Acquir Immune Defic Syndr. 2015;69:278-85 pubmed publisher
Iyer S, Agrawal R, Thompson C, Thompson S, Barton J, Kusner D. Phospholipase D1 regulates phagocyte adhesion. J Immunol. 2006;176:3686-96 pubmed
Tan S, Robinson M, Drbal K, van Kooyk Y, Shaw J, Law S. The N-terminal region and the mid-region complex of the integrin beta 2 subunit. J Biol Chem. 2001;276:36370-6 pubmed
Hogg N, Stewart M, Scarth S, Newton R, Shaw J, Law S, et al. A novel leukocyte adhesion deficiency caused by expressed but nonfunctional beta2 integrins Mac-1 and LFA-1. J Clin Invest. 1999;103:97-106 pubmed
Douglass W, Hyland R, Buckley C, Al Shamkhani A, Shaw J, Scarth S, et al. The role of the cysteine-rich region of the beta2 integrin subunit in the leukocyte function-associated antigen-1 (LFA-1, alphaLbeta2, CD11a/CD18) heterodimer formation and ligand binding. FEBS Lett. 1998;440:414-8 pubmed
Berman P, Nakamura G, Riddle L, Chiu H, Fisher K, Champe M, et al. Biosynthesis and function of membrane bound and secreted forms of recombinant CD11b/CD18 (Mac-1). J Cell Biochem. 1993;52:183-95 pubmed
Hildreth J, August J. The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): functional effects of subunit-specific monoclonal antibodies. J Immunol. 1985;134:3272-80 pubmed
Hildreth J, Orentas R. Involvement of a leukocyte adhesion receptor (LFA-1) in HIV-induced syncytium formation. Science. 1989;244:1075-8 pubmed
Hildreth J, Holt V, August J, Pescovitz M. Monoclonal antibodies against porcine LFA-1: species cross-reactivity and functional effects of beta-subunit-specific antibodies. Mol Immunol. 1989;26:883-95 pubmed
Kansas G, Wood G, Tedder T. Expression, distribution, and biochemistry of human CD39. Role in activation-associated homotypic adhesion of lymphocytes. J Immunol. 1991;146:2235-44 pubmed
Stocks S, Kerr M. Stimulation of neutrophil adhesion by antibodies recognizing CD15 (Le(X)) and CD15-expressing carcinoembryonic antigen-related glycoprotein NCA-160. Biochem J. 1992;288 ( Pt 1):23-7 pubmed

product information

Internal ID :

4125

Name :

H52

Depositor Name :

Hildreth, J.E.K.

Depositor Institution :

Johns Hopkins University School of Medicine

Date Deposited :

3/28/88

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

CD18 (integrin beta-2; beta subunit of CD11a, b, c), human

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Canine,Human,Pig

Species Tested (legacy) :

human, pig, dog; not reactive with mouse, rat, hamster, rabbit, cow

Initial Publication Pubmed ID :

2580022

Depositor Notes (Special Instructions) :

Does not cross react with mouse, rat, hamster, rabbit, cow. Blocks leukocyte adhesion and inhibits T cell and NK cell activity. Reactive with frozen sections of human tonsil. Binds all leukocytes.

Collections :

CD antigens,Cell adhesion,Cell markers,Cell signaling,Human

Search Keywords :

Hildreth, J.E.K., CD18 (integrin beta-2; beta subunit of CD11a, b, c), human, Human, MIgG1, kappa light chain, Human/Pig/Canine, ITGB2, CD18, MFI7, LAD, LCAMB, LFA-1, MAC-1, AB_528137, monoclonal, Human/Cell signaling/Cluster Determinant antigens/Cell adhesion/Cell markers, Immunohistochemistry/Immunoprecipitation/Western Blot/Function Blocking/FACS

Antigen Molecular Weight :

Predicted: 85 kDa; Apparent: 93 kDa

Gene :

ITGB2

Alternate Gene Name(s) :

CD18, MFI7, LAD, LCAMB, LFA-1, MAC-1

Uniprot ID :

P05107

Antibody Registry ID :

AB_528137

Synonyms (Alternate Clone Names) :

CD18 (beta subunit of CD11a, b, c)

Immunogen :

Human splenic adherent cells .

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3x653-Ag8

Epitope Mapped :

Yes

Epitope Location or Sequence :

Mid-region (aa 362-460)

Epitope Map PubMed ID :

11477072

Recommended Applications :

FACS,Function Blocking,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

2580022

Immunoprecipitation Pubmed IDs :

2580022 2481234 9872413 8366136

Function Blocking Pubmed IDs :

2580022 25828964 16517737 2481234 2543075 1672348 1359882 8366136

FACS Pubmed IDs :

2481234 9872413 9884339

Additional Information :

CD18 is a general marker of leukocytes.

DSHB Growth Medium :

Iscove's

References (legacy) :

Eur. J. Immunol. 13, 202-208.; J. Immunol. 134, 3272-3280.; Eur. J. Immunol. 15, 888-892.; EMBO J. 6, 915-919.; Mol. Immunol. 26, 883-895.; Science 244, 1075-1078.

more info or order :

Developmental Studies Hybridoma Bank product webpage