product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
LAMP-1 (human)
catalog :
H4A3
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
H4A3
reactivity :
fission yeast, human, mouse, rat, dogs
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, other
citations: 162
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; loading ...; fig 1c
Prabhu A, Kang I, De Pace R, Wassif C, Fujiwara H, Kell P, et al. A human iPSC-derived inducible neuronal model of Niemann-Pick disease, type C1. BMC Biol. 2021;19:218 pubmed publisher
  • immunocytochemistry; human; 1:400; loading ...; fig s3a
Mathieu M, Nevo N, Jouve M, Valenzuela J, Maurin M, Verweij F, et al. Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. Nat Commun. 2021;12:4389 pubmed publisher
  • immunocytochemistry; human; fig 5c
Takahashi K, Kanerva K, Vanharanta L, Almeida Souza L, Lietha D, Olkkonen V, et al. ORP2 couples LDL-cholesterol transport to FAK activation by endosomal cholesterol/PI(4,5)P2 exchange. EMBO J. 2021;40:e106871 pubmed publisher
  • other; human; 1:10; loading ...; fig s1h
Hess M, Krainer I, Filipek P, Witting B, Gutleben K, Vietor I, et al. Advanced Microscopy for Liver and Gut Ultrastructural Pathology in Patients with MVID and PFIC Caused by MYO5B Mutations. J Clin Med. 2021;10: pubmed publisher
  • western blot; human; 1:5000; loading ...; fig s5b
Caballero B, Bourdenx M, Luengo E, Díaz A, Sohn P, Chen X, et al. Acetylated tau inhibits chaperone-mediated autophagy and promotes tau pathology propagation in mice. Nat Commun. 2021;12:2238 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 4g
Ben Sasson A, Watson J, Sheffler W, Johnson M, Bittleston A, Somasundaram L, et al. Design of biologically active binary protein 2D materials. Nature. 2021;589:468-473 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2c
Liao Y, Fernandopulle M, Wang G, Choi H, Hao L, Drerup C, et al. RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, Using Annexin A11 as a Molecular Tether. Cell. 2019;179:147-164.e20 pubmed publisher
  • western blot; human; loading ...; fig 1h
Xiao J, Luo J, Hu A, Xiao T, Li M, Kong Z, et al. Cholesterol transport through the peroxisome-ER membrane contacts tethered by PI(4,5)P2 and extended synaptotagmins. Sci China Life Sci. 2019;: pubmed publisher
  • immunocytochemistry; human; loading ...; fig s1f
Quinney K, Frankel E, Shankar R, Kasberg W, Luong P, Audhya A. Growth factor stimulation promotes multivesicular endosome biogenesis by prolonging recruitment of the late-acting ESCRT machinery. Proc Natl Acad Sci U S A. 2019;116:6858-6867 pubmed publisher
  • immunocytochemistry; human; 2 ug/ml; loading ...; fig s1d
Bae D, Moore K, Mella J, Hayashi S, Hollien J. Degradation of Blos1 mRNA by IRE1 repositions lysosomes and protects cells from stress. J Cell Biol. 2019;218:1118-1127 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig 1e
Skowyra M, Schlesinger P, Naismith T, Hanson P. Triggered recruitment of ESCRT machinery promotes endolysosomal repair. Science. 2018;360: pubmed publisher
  • immunocytochemistry; human; fig s1e
Takada N, Naito T, Inoue T, Nakayama K, Takatsu H, Shin H. Phospholipid-flipping activity of P4-ATPase drives membrane curvature. EMBO J. 2018;37: pubmed publisher
  • western blot; human; loading ...; fig 5d
Marrone L, Bus C, Schöndorf D, Fitzgerald J, Kübler M, Schmid B, et al. Generation of iPSCs carrying a common LRRK2 risk allele for in vitro modeling of idiopathic Parkinson's disease. PLoS ONE. 2018;13:e0192497 pubmed publisher
  • immunocytochemistry; mouse; 1:250; loading ...; fig 9d
Krey J, Dumont R, Wilmarth P, David L, Johnson K, Barr Gillespie P. ELMOD1 Stimulates ARF6-GTP Hydrolysis to Stabilize Apical Structures in Developing Vestibular Hair Cells. J Neurosci. 2018;38:843-857 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig s4d
Jimenez Orgaz A, Kvainickas A, Nägele H, Denner J, Eimer S, Dengjel J, et al. Control of RAB7 activity and localization through the retromer-TBC1D5 complex enables RAB7-dependent mitophagy. EMBO J. 2018;37:235-254 pubmed publisher
  • immunocytochemistry; human; 1:5; fig 7c
Shiba Fukushima K, Ishikawa K, Inoshita T, Izawa N, Takanashi M, Sato S, et al. Evidence that phosphorylated ubiquitin signaling is involved in the etiology of Parkinson's disease. Hum Mol Genet. 2017;26:3172-3185 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 7a
Wilhelm L, Wendling C, Vedie B, Kobayashi T, Chenard M, Tomasetto C, et al. STARD3 mediates endoplasmic reticulum-to-endosome cholesterol transport at membrane contact sites. EMBO J. 2017;36:1412-1433 pubmed publisher
  • western blot; human; loading ...; fig 10c
Jung J, Nayak A, Schaeffer V, Starzetz T, Kirsch A, Muller S, et al. Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator. elife. 2017;6: pubmed publisher
  • western blot; human; 1:400; loading ...; fig 5b
Borgia D, Malena A, Spinazzi M, Desbats M, Salviati L, Russell A, et al. Increased mitophagy in the skeletal muscle of spinal and bulbar muscular atrophy patients. Hum Mol Genet. 2017;26:1087-1103 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1c
Cianciola N, Chung S, Manor D, Carlin C. Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum. J Virol. 2017;91: pubmed publisher
  • western blot; human; loading ...; fig 6e
Shi B, Huang Q, Birkett R, Doyle R, Dorfleutner A, Stehlik C, et al. SNAPIN is critical for lysosomal acidification and autophagosome maturation in macrophages. Autophagy. 2017;13:285-301 pubmed publisher
  • flow cytometry; human; loading ...; fig s6d
Galindo Albarrán A, López Portales O, Gutiérrez Reyna D, Rodríguez Jorge O, Sánchez Villanueva J, Ramirez Pliego O, et al. CD8+ T Cells from Human Neonates Are Biased toward an Innate Immune Response. Cell Rep. 2016;17:2151-2160 pubmed publisher
  • immunocytochemistry; mouse; 1:300; fig 3
Jaber N, Mohd Naim N, Wang Z, Deleon J, Kim S, Zhong H, et al. Vps34 regulates Rab7 and late endocytic trafficking through recruitment of the GTPase-activating protein Armus. J Cell Sci. 2016;129:4424-4435 pubmed
  • immunocytochemistry; human; loading ...; fig 2a
Rofe A, Davis L, Whittingham J, Latimer Bowman E, Wilkinson A, Pryor P. The Rhodococcus equi virulence protein VapA disrupts endolysosome function and stimulates lysosome biogenesis. Microbiologyopen. 2017;6: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 6a
Roberts B, Svoboda R, Overmiller A, Lewis J, Kowalczyk A, Mahoney M, et al. Palmitoylation of Desmoglein 2 Is a Regulator of Assembly Dynamics and Protein Turnover. J Biol Chem. 2016;291:24857-24865 pubmed
  • immunocytochemistry; mouse; 1:200; loading ...; fig 2b
Rahman N, Ramos Espiritu L, Milner T, Buck J, Levin L. Soluble adenylyl cyclase is essential for proper lysosomal acidification. J Gen Physiol. 2016;148:325-39 pubmed publisher
  • immunocytochemistry; dogs; fig 4a
Dolat L, Spiliotis E. Septins promote macropinosome maturation and traffic to the lysosome by facilitating membrane fusion. J Cell Biol. 2016;214:517-27 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2d
McMillan K, Gallon M, Jellett A, Clairfeuille T, Tilley F, McGough I, et al. Atypical parkinsonism-associated retromer mutant alters endosomal sorting of specific cargo proteins. J Cell Biol. 2016;214:389-99 pubmed publisher
  • western blot; human; 1:1000; fig 6a
Zhang X, Cheng X, Yu L, Yang J, Calvo R, Patnaik S, et al. MCOLN1 is a ROS sensor in lysosomes that regulates autophagy. Nat Commun. 2016;7:12109 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 2b
Wu X, Zhao L, Chen Z, Ji X, Qiao X, Jin Y, et al. FLCN Maintains the Leucine Level in Lysosome to Stimulate mTORC1. PLoS ONE. 2016;11:e0157100 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1a
Starling G, Yip Y, Sanger A, Morton P, Eden E, Dodding M. Folliculin directs the formation of a Rab34-RILP complex to control the nutrient-dependent dynamic distribution of lysosomes. EMBO Rep. 2016;17:823-41 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 7g
Li Z, Ji X, Wang W, Liu J, Liang X, Wu H, et al. Ammonia Induces Autophagy through Dopamine Receptor D3 and MTOR. PLoS ONE. 2016;11:e0153526 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1000; loading ...; tbl 1
Ito M, Nakamura K, Mori F, Miki Y, Tanji K, Wakabayashi K. Novel eosinophilic neuronal cytoplasmic inclusions in the external cuneate nucleus of humans. Neuropathology. 2016;36:441-447 pubmed publisher
  • immunocytochemistry; human; fig s2
Hatori Y, Yan Y, Schmidt K, Furukawa E, Hasan N, Yang N, et al. Neuronal differentiation is associated with a redox-regulated increase of copper flow to the secretory pathway. Nat Commun. 2016;7:10640 pubmed publisher
  • western blot; human; loading ...; fig 6a
Pérez L, McLetchie S, Gardiner G, Deffit S, Zhou D, Blum J. LAMP-2C Inhibits MHC Class II Presentation of Cytoplasmic Antigens by Disrupting Chaperone-Mediated Autophagy. J Immunol. 2016;196:2457-65 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 2
Circu M, Dykes S, Carroll J, Kelly K, Galiano F, Greer A, et al. A Novel High Content Imaging-Based Screen Identifies the Anti-Helminthic Niclosamide as an Inhibitor of Lysosome Anterograde Trafficking and Prostate Cancer Cell Invasion. PLoS ONE. 2016;11:e0146931 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1a
Zhang S, Schneider L, Vick B, Grunert M, Jeremias I, Menche D, et al. Anti-leukemic effects of the V-ATPase inhibitor Archazolid A. Oncotarget. 2015;6:43508-28 pubmed publisher
  • immunohistochemistry; human; 1:100
Scifo E, Szwajda A, Soliymani R, Pezzini F, Bianchi M, Dapkunas A, et al. Quantitative analysis of PPT1 interactome in human neuroblastoma cells. Data Brief. 2015;4:207-16 pubmed publisher
  • immunocytochemistry; human; fig s4a
Hobbs R, DePianto D, Jacob J, Han M, Chung B, Batazzi A, et al. Keratin-dependent regulation of Aire and gene expression in skin tumor keratinocytes. Nat Genet. 2015;47:933-8 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 6
Costantini L, Baloban M, Markwardt M, Rizzo M, Guo F, Verkhusha V, et al. A palette of fluorescent proteins optimized for diverse cellular environments. Nat Commun. 2015;6:7670 pubmed publisher
  • immunocytochemistry; human; 1:250; fig 4
Cao Q, Zhong X, Zou Y, Murrell Lagnado R, Zhu M, Dong X. Calcium release through P2X4 activates calmodulin to promote endolysosomal membrane fusion. J Cell Biol. 2015;209:879-94 pubmed publisher
  • immunocytochemistry; human; 1:200; fig s6
  • western blot; human; 1:500; fig s7
Akizu N, Cantagrel V, Zaki M, Al Gazali L, Wang X, Rosti R, et al. Biallelic mutations in SNX14 cause a syndromic form of cerebellar atrophy and lysosome-autophagosome dysfunction. Nat Genet. 2015;47:528-34 pubmed publisher
  • immunocytochemistry; human; fig 5
  • western blot; human; fig 5
Brandstaetter H, Kishi Itakura C, Tumbarello D, Manstein D, Buss F. Loss of functional MYO1C/myosin 1c, a motor protein involved in lipid raft trafficking, disrupts autophagosome-lysosome fusion. Autophagy. 2014;10:2310-23 pubmed publisher
  • immunocytochemistry; human; fig 4, 5
Ram S, Kim D, Ober R, Ward E. The level of HER2 expression is a predictor of antibody-HER2 trafficking behavior in cancer cells. MAbs. 2014;6:1211-9 pubmed publisher
  • immunocytochemistry; human; fig s9f
Boucrot E, Ferreira A, Almeida Souza L, Debard S, Vallis Y, Howard G, et al. Endophilin marks and controls a clathrin-independent endocytic pathway. Nature. 2015;517:460-5 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 5
Bohnsack R, Warejcka D, Wang L, Gillespie S, Bernstein A, Twining S, et al. Expression of insulin-like growth factor 2 receptor in corneal keratocytes during differentiation and in response to wound healing. Invest Ophthalmol Vis Sci. 2014;55:7697-708 pubmed publisher
  • immunocytochemistry; human
Ye S, Huang Y, Joshi S, Zhang J, Yang F, Zhang G, et al. Platelet secretion and hemostasis require syntaxin-binding protein STXBP5. J Clin Invest. 2014;124:4517-28 pubmed publisher
  • immunocytochemistry; human; fig 2, 3, 4
Menhofer M, Bartel D, Liebl J, Kubisch R, Busse J, Wagner E, et al. In vitro and in vivo characterization of the actin polymerizing compound chondramide as an angiogenic inhibitor. Cardiovasc Res. 2014;104:303-14 pubmed publisher
  • flow cytometry; human; fig 2
Madhavi V, Ana Sosa Batiz F, Jegaskanda S, Center R, Winnall W, Parsons M, et al. Antibody-dependent effector functions against HIV decline in subjects receiving antiretroviral therapy. J Infect Dis. 2015;211:529-38 pubmed publisher
  • immunocytochemistry; fission yeast; fig s4
Cheng J, Fujita A, Yamamoto H, Tatematsu T, Kakuta S, Obara K, et al. Yeast and mammalian autophagosomes exhibit distinct phosphatidylinositol 3-phosphate asymmetries. Nat Commun. 2014;5:3207 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1000
Kon T, Mori F, Tanji K, Miki Y, Kimura T, Wakabayashi K. Giant cell polymyositis and myocarditis associated with myasthenia gravis and thymoma. Neuropathology. 2013;33:281-7 pubmed publisher
  • immunocytochemistry; human
Wild P, Farhan H, McEwan D, Wagner S, Rogov V, Brady N, et al. Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth. Science. 2011;333:228-33 pubmed publisher
  • immunocytochemistry; human
Yu X, McGourty K, Liu M, Unsworth K, Holden D. pH sensing by intracellular Salmonella induces effector translocation. Science. 2010;328:1040-3 pubmed publisher
  • western blot; human
Howe D, Heinzen R. Fractionation of the Coxiella burnetii parasitophorous vacuole. Methods Mol Biol. 2008;445:389-406 pubmed publisher
Chong A, Starr T, Finn C, Steele Mortimer O. A role for the Salmonella Type III Secretion System 1 in bacterial adaptation to the cytosol of epithelial cells. Mol Microbiol. 2019;112:1270-1283 pubmed publisher
Piccolo P, Sabatino V, Mithbaokar P, Polishchuk E, Hicks J, Polishchuk R, et al. Skin fibroblasts of patients with geleophysic dysplasia due to FBN1 mutations have lysosomal inclusions and losartan improves their microfibril deposition defect. Mol Genet Genomic Med. 2019;7:e844 pubmed publisher
Byndloss M, Tsai A, Walker G, Miller C, Young B, English B, et al. Brucella abortus Infection of Placental Trophoblasts Triggers Endoplasmic Reticulum Stress-Mediated Cell Death and Fetal Loss via Type IV Secretion System-Dependent Activation of CHOP. MBio. 2019;10: pubmed publisher
Stathakos P, Jiménez Moreno N, Crompton L, Nistor P, Caldwell M, Lane J. Imaging Autophagy in hiPSC-Derived Midbrain Dopaminergic Neuronal Cultures for Parkinson's Disease Research. Methods Mol Biol. 2019;1880:257-280 pubmed publisher
Murakami K, Etlinger J. Role of SMURF1 ubiquitin ligase in BMP receptor trafficking and signaling. Cell Signal. 2019;54:139-149 pubmed publisher
Augustin H, McGourty K, Allen M, Madem S, Adcott J, Kerr F, et al. Reduced insulin signaling maintains electrical transmission in a neural circuit in aging flies. PLoS Biol. 2017;15:e2001655 pubmed publisher
Bekier M, Wang L, Li J, Huang H, Tang D, Zhang X, et al. Knockout of the Golgi stacking proteins GRASP55 and GRASP65 impairs Golgi structure and function. Mol Biol Cell. 2017;28:2833-2842 pubmed publisher
Totland M, Bergsland C, Fykerud T, Knudsen L, Rasmussen N, Eide P, et al. The E3 ubiquitin ligase NEDD4 induces endocytosis and lysosomal sorting of connexin 43 to promote loss of gap junctions. J Cell Sci. 2017;130:2867-2882 pubmed publisher
Tournu H, Carroll J, Latimer B, Dragoi A, Dykes S, Cardelli J, et al. Identification of small molecules that disrupt vacuolar function in the pathogen Candida albicans. PLoS ONE. 2017;12:e0171145 pubmed publisher
Zhou M, Wiener H, Su W, Zhou Y, Liot C, Ahearn I, et al. VPS35 binds farnesylated N-Ras in the cytosol to regulate N-Ras trafficking. J Cell Biol. 2016;214:445-58 pubmed publisher
Kohler L, Reed S, Sarraf S, Arteaga D, Newton H, Roy C. Effector Protein Cig2 Decreases Host Tolerance of Infection by Directing Constitutive Fusion of Autophagosomes with the Coxiella-Containing Vacuole. MBio. 2016;7: pubmed publisher
Bento C, Ashkenazi A, Jimenez Sanchez M, Rubinsztein D. The Parkinson's disease-associated genes ATP13A2 and SYT11 regulate autophagy via a common pathway. Nat Commun. 2016;7:11803 pubmed publisher
Marin Zapata P, Beese C, Jünger A, Dalmasso G, Brady N, Hamacher Brady A. Time course decomposition of cell heterogeneity in TFEB signaling states reveals homeostatic mechanisms restricting the magnitude and duration of TFEB responses to mTOR activity modulation. BMC Cancer. 2016;16:355 pubmed publisher
Mell J, Viadas C, Moleres J, Sinha S, Fernández Calvet A, Porsch E, et al. Transformed Recombinant Enrichment Profiling Rapidly Identifies HMW1 as an Intracellular Invasion Locus in Haemophilus influenza. PLoS Pathog. 2016;12:e1005576 pubmed publisher
O Brien M, Manches O, WILEN C, Gopal R, Huq R, Wu V, et al. CD4 Receptor is a Key Determinant of Divergent HIV-1 Sensing by Plasmacytoid Dendritic Cells. PLoS Pathog. 2016;12:e1005553 pubmed publisher
Johnson D, Ostrowski P, Jaumouillé V, Grinstein S. The position of lysosomes within the cell determines their luminal pH. J Cell Biol. 2016;212:677-92 pubmed publisher
Rodríguez Escudero M, Cid V, Molina M, Schulze Luehrmann J, Lührmann A, Rodríguez Escudero I. Studying Coxiella burnetii Type IV Substrates in the Yeast Saccharomyces cerevisiae: Focus on Subcellular Localization and Protein Aggregation. PLoS ONE. 2016;11:e0148032 pubmed publisher
Kilpatrick B, Magalhães J, Beavan M, McNeill A, Gegg M, Cleeter M, et al. Endoplasmic reticulum and lysosomal Ca²⁺ stores are remodelled in GBA1-linked Parkinson disease patient fibroblasts. Cell Calcium. 2016;59:12-20 pubmed publisher
Dou Z, Xu C, Donahue G, Shimi T, Pan J, Zhu J, et al. Autophagy mediates degradation of nuclear lamina. Nature. 2015;527:105-9 pubmed publisher
Evans G, Caller L, Foster V, Crump C. Anion homeostasis is important for non-lytic release of BK polyomavirus from infected cells. Open Biol. 2015;5: pubmed publisher
Prado M, Eickel N, De Niz M, Heitmann A, Agop Nersesian C, Wacker R, et al. Long-term live imaging reveals cytosolic immune responses of host hepatocytes against Plasmodium infection and parasite escape mechanisms. Autophagy. 2015;11:1561-79 pubmed publisher
Wan M, Zhang W, Tian Y, Xu C, Xu T, Liu J, et al. Unraveling a molecular determinant for clathrin-independent internalization of the M2 muscarinic acetylcholine receptor. Sci Rep. 2015;5:11408 pubmed publisher
Rasmussen J, Fletcher J, Long M, Allen L, Jones B. Characterization of Francisella tularensis Schu S4 mutants identified from a transposon library screened for O-antigen and capsule deficiencies. Front Microbiol. 2015;6:338 pubmed publisher
Venè R, Tosetti F, Minghelli S, Poggi A, Ferrari N, Benelli R. Celecoxib increases EGF signaling in colon tumor associated fibroblasts, modulating EGFR expression and degradation. Oncotarget. 2015;6:12310-25 pubmed
Santiago Tirado F, Peng T, Yang M, Hang H, Doering T. A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome. PLoS Pathog. 2015;11:e1004908 pubmed publisher
Dirk B, Jacob R, Johnson A, Pawlak E, Cavanagh P, Van Nynatten L, et al. Viral bimolecular fluorescence complementation: a novel tool to study intracellular vesicular trafficking pathways. PLoS ONE. 2015;10:e0125619 pubmed publisher
Nakatsu F, Messa M, Nández R, Czapla H, Zou Y, Strittmatter S, et al. Sac2/INPP5F is an inositol 4-phosphatase that functions in the endocytic pathway. J Cell Biol. 2015;209:85-95 pubmed publisher
Roboti P, Sato K, Lowe M. The golgin GMAP-210 is required for efficient membrane trafficking in the early secretory pathway. J Cell Sci. 2015;128:1595-606 pubmed publisher
Cortes Canteli M, Mattei L, Richards A, Norris E, Strickland S. Fibrin deposited in the Alzheimer's disease brain promotes neuronal degeneration. Neurobiol Aging. 2015;36:608-17 pubmed publisher
Hockey L, Kilpatrick B, Eden E, Lin Moshier Y, Brailoiu G, Brailoiu E, et al. Dysregulation of lysosomal morphology by pathogenic LRRK2 is corrected by TPC2 inhibition. J Cell Sci. 2015;128:232-8 pubmed publisher
Canton J, Khezri R, Glogauer M, Grinstein S. Contrasting phagosome pH regulation and maturation in human M1 and M2 macrophages. Mol Biol Cell. 2014;25:3330-41 pubmed publisher
Newton H, Kohler L, McDonough J, Temoche Diaz M, Crabill E, Hartland E, et al. A screen of Coxiella burnetii mutants reveals important roles for Dot/Icm effectors and host autophagy in vacuole biogenesis. PLoS Pathog. 2014;10:e1004286 pubmed publisher
Mangieri L, Mader B, Thomas C, Taylor C, Luker A, Tse T, et al. ATP6V0C knockdown in neuroblastoma cells alters autophagy-lysosome pathway function and metabolism of proteins that accumulate in neurodegenerative disease. PLoS ONE. 2014;9:e93257 pubmed publisher
Palibrk V, Lång E, Lang A, Schink K, Rowe A, Bøe S. Promyelocytic leukemia bodies tether to early endosomes during mitosis. Cell Cycle. 2014;13:1749-55 pubmed publisher
Steffan J, Dykes S, Coleman D, Adams L, Rogers D, Carroll J, et al. Supporting a role for the GTPase Rab7 in prostate cancer progression. PLoS ONE. 2014;9:e87882 pubmed publisher
Valapala M, Wilson C, Hose S, Bhutto I, Grebe R, Dong A, et al. Lysosomal-mediated waste clearance in retinal pigment epithelial cells is regulated by CRYBA1/?A3/A1-crystallin via V-ATPase-MTORC1 signaling. Autophagy. 2014;10:480-96 pubmed publisher
Hannemann S, Gao B, Galan J. Salmonella modulation of host cell gene expression promotes its intracellular growth. PLoS Pathog. 2013;9:e1003668 pubmed publisher
Schowalter R, Buck C. The Merkel cell polyomavirus minor capsid protein. PLoS Pathog. 2013;9:e1003558 pubmed publisher
Jensen S, Aaberg Jessen C, Christensen K, Kristensen B. Expression of the lysosomal-associated membrane protein-1 (LAMP-1) in astrocytomas. Int J Clin Exp Pathol. 2013;6:1294-305 pubmed
Renna M, Bento C, Fleming A, Menzies F, Siddiqi F, Ravikumar B, et al. IGF-1 receptor antagonism inhibits autophagy. Hum Mol Genet. 2013;22:4528-44 pubmed publisher
Sudhan D, Siemann D. Cathepsin L inhibition by the small molecule KGP94 suppresses tumor microenvironment enhanced metastasis associated cell functions of prostate and breast cancer cells. Clin Exp Metastasis. 2013;30:891-902 pubmed publisher
Weflen A, Baier N, Tang Q, Van den Hof M, Blumberg R, Lencer W, et al. Multivalent immune complexes divert FcRn to lysosomes by exclusion from recycling sorting tubules. Mol Biol Cell. 2013;24:2398-405 pubmed publisher
Faron M, Fletcher J, Rasmussen J, Long M, Allen L, Jones B. The Francisella tularensis migR, trmE, and cphA genes contribute to F. tularensis pathogenicity island gene regulation and intracellular growth by modulation of the stress alarmone ppGpp. Infect Immun. 2013;81:2800-11 pubmed publisher
Notte A, Ninane N, Arnould T, Michiels C. Hypoxia counteracts taxol-induced apoptosis in MDA-MB-231 breast cancer cells: role of autophagy and JNK activation. Cell Death Dis. 2013;4:e638 pubmed publisher
Agrawal T, Schu P, Medigeshi G. Adaptor protein complexes-1 and 3 are involved at distinct stages of flavivirus life-cycle. Sci Rep. 2013;3:1813 pubmed publisher
Figueira R, Watson K, Holden D, Helaine S. Identification of salmonella pathogenicity island-2 type III secretion system effectors involved in intramacrophage replication of S. enterica serovar typhimurium: implications for rational vaccine design. MBio. 2013;4:e00065 pubmed publisher
Xiang Y, Zhang X, Nix D, Katoh T, Aoki K, Tiemeyer M, et al. Regulation of protein glycosylation and sorting by the Golgi matrix proteins GRASP55/65. Nat Commun. 2013;4:1659 pubmed publisher
Chao J, Ram S, Ward E, Ober R. Ultrahigh accuracy imaging modality for super-localization microscopy. Nat Methods. 2013;10:335-8 pubmed publisher
Chapel A, Kieffer Jaquinod S, Sagné C, Verdon Q, Ivaldi C, Mellal M, et al. An extended proteome map of the lysosomal membrane reveals novel potential transporters. Mol Cell Proteomics. 2013;12:1572-88 pubmed publisher
Martina J, Puertollano R. Rag GTPases mediate amino acid-dependent recruitment of TFEB and MITF to lysosomes. J Cell Biol. 2013;200:475-91 pubmed publisher
McDonough J, Newton H, Klum S, Swiss R, Agaisse H, Roy C. Host pathways important for Coxiella burnetii infection revealed by genome-wide RNA interference screening. MBio. 2013;4:e00606-12 pubmed publisher
Del Giudice M, Ugalde J, Czibener C. A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication. Infect Immun. 2013;81:956-64 pubmed publisher
Fernandes M, Flannery A, Andrews N, Mortara R. Extracellular amastigotes of Trypanosoma cruzi are potent inducers of phagocytosis in mammalian cells. Cell Microbiol. 2013;15:977-91 pubmed publisher
Roset M, García Fernández L, DelVecchio V, Briones G. Intracellularly induced cyclophilins play an important role in stress adaptation and virulence of Brucella abortus. Infect Immun. 2013;81:521-30 pubmed publisher
Bello Morales R, Crespillo A, Fraile Ramos A, Tabarés E, Alcina A, Lopez Guerrero J. Role of the small GTPase Rab27a during herpes simplex virus infection of oligodendrocytic cells. BMC Microbiol. 2012;12:265 pubmed publisher
Verschooten L, Barrette K, Van Kelst S, Rubio Romero N, Proby C, De Vos R, et al. Autophagy inhibitor chloroquine enhanced the cell death inducing effect of the flavonoid luteolin in metastatic squamous cell carcinoma cells. PLoS ONE. 2012;7:e48264 pubmed publisher
Yeh T, Quintyne N, Scipioni B, Eckley D, Schroer T. Dynactin's pointed-end complex is a cargo-targeting module. Mol Biol Cell. 2012;23:3827-37 pubmed publisher
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product information
Internal ID :
503
Name :
H4A3
Depositor Name :
August, J.T. / Hildreth, J.E.K.
Depositor Institution :
The Johns Hopkins University School of Medicine
Date Deposited :
4/12/93
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
LAMP-1 (human)
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Hamster,Human,Primate,Rat
Species Tested (legacy) :
human, hamster, monkey, rat
Initial Publication Pubmed ID :
2409098
Depositor Notes (Special Instructions) :
This antibody recognizes a membrane glycoprotein which interacts with selectins.
Collections :
CD antigens,Cell signaling,Human
Search Keywords :
August, J.T. / Hildreth, J.E.K., Lysosomal-associated membrane protein 1; Lamp-1 ; CD107a, MIgG1, Hamster/Human/Primate/Rat, LAMP1, , AB_2296838, Monoclonal, LAMP1, LGP120, LAMPA, Immunofluorescence,Immunohistochemistry,Western Blot
Antigen Molecular Weight :
Predicted: 42 kDa; Apparent: 120 kDa
Gene :
LAMP1
Alternate Gene Name(s) :
CD107a, LGP120, LAMPA
Uniprot ID :
P11279
Antibody Registry ID :
AB_2296838
Synonyms (Alternate Clone Names) :
CD107a
Immunogen :
Adherent spleen cells
Clonality :
Monoclonal
Myeloma Strain :
P3X653-Ag8
Epitope Mapped :
Yes
Epitope Location or Sequence :
Luminal region
Epitope Map PubMed ID :
11511344
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
23826410 15944902 31350823
Immunofluorescence Pubmed IDs :
25080348 23748470 23716606 23241026 23436907 23319555 23230297 23681233 23362322 22505584 27435465 30395943 30610703 31337727 31370104
Western Blot Pubmed IDs :
28814501 22070883 20173215 19910472 19286132 18817525 16565504 12021262 11342594 11441007
Pubmed IDs :
25475538
Additional Information :
For experiments involving live cells and tissue, H4A3 supernatant is available without the antimicrobial ProClin. Contact us at 319-335-3826 or dshb@uiowa.edu for special ordering instructions.
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Cell Biol. 101, 85-95.; Biochem. Soc. Symp. 51, 97-112.; J. Biol. Chem. 263, 8754-8758.; Arch. Biochem. Biophys. 268, 360-378.; J. Biol. Chem. 265, 5008-5013.; J. Biol. Chem. 265, 7419-7423.; J. Biol. Chem. 275(12), 8672-8679.; EMBO J. 23, 4560-4570.; Acta. Neuropathol. 108, 287-294.; Mol. Biol. Cell 15, 5420-5430.; Autophagy, 1(1), 23-36.; J. Cell. Sci. 119, 2003-2014.; Am. J. Pathol. 168 (4), 1309-1320.; Am. J. Hum. Genet. 81, 136-146.; BMC Cell Biology 10:84 doi:10.1186/1471-2121-10-84.; J. Biol. Chem. 286(33), 29166-29174.; J. Neurochem. 123, 298-309.; J. Cell Sci. 126(1), 60-66.; Neurobiol. Aging, In press. http://dx.doi.org/10.1016/j.neurobiolaging.2014.10.030.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US