product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
synuclein, alpha & beta
catalog :
H3C
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
H3C
reactivity :
common canary, fruit fly
application :
western blot, immunocytochemistry, immunoprecipitation
citations: 18
Published Application/Species/Sample/DilutionReference
  • western blot; fruit fly; 1:1000; loading ...; fig s1a
Bangi E, Murgia C, Teague A, Sansom O, Cagan R. Functional exploration of colorectal cancer genomes using Drosophila. Nat Commun. 2016;7:13615 pubmed publisher
Ordonez D, Lee M, Feany M. α-synuclein Induces Mitochondrial Dysfunction through Spectrin and the Actin Cytoskeleton. Neuron. 2018;97:108-124.e6 pubmed publisher
Schwab A, Ebert A. Neurite Aggregation and Calcium Dysfunction in iPSC-Derived Sensory Neurons with Parkinson's Disease-Related LRRK2 G2019S Mutation. Stem Cell Reports. 2015;5:1039-1052 pubmed publisher
Chu W, Zhou D, Gaba V, Liu J, Li S, Peng X, et al. Design, Synthesis, and Characterization of 3-(Benzylidene)indolin-2-one Derivatives as Ligands for α-Synuclein Fibrils. J Med Chem. 2015;58:6002-17 pubmed publisher
Neal K, Shakerdge N, Hou S, Klunk W, Mathis C, Nesterov E, et al. Development and screening of contrast agents for in vivo imaging of Parkinson's disease. Mol Imaging Biol. 2013;15:585-95 pubmed publisher
Boassa D, Berlanga M, Yang M, Terada M, Hu J, Bushong E, et al. Mapping the subcellular distribution of ?-synuclein in neurons using genetically encoded probes for correlated light and electron microscopy: implications for Parkinson's disease pathogenesis. J Neurosci. 2013;33:2605-15 pubmed publisher
Klucken J, Poehler A, Ebrahimi Fakhari D, Schneider J, Nuber S, Rockenstein E, et al. Alpha-synuclein aggregation involves a bafilomycin A 1-sensitive autophagy pathway. Autophagy. 2012;8:754-66 pubmed publisher
Perrin R, Payton J, Barnett D, Wraight C, Woods W, Ye L, et al. Epitope mapping and specificity of the anti-alpha-synuclein monoclonal antibody Syn-1 in mouse brain and cultured cell lines. Neurosci Lett. 2003;349:133-5 pubmed
Payton J, Perrin R, Clayton D, George J. Protein-protein interactions of alpha-synuclein in brain homogenates and transfected cells. Brain Res Mol Brain Res. 2001;95:138-45 pubmed
Perrin R, Woods W, Clayton D, George J. Exposure to long chain polyunsaturated fatty acids triggers rapid multimerization of synucleins. J Biol Chem. 2001;276:41958-62 pubmed
McLean P, Kawamata H, Hyman B. Alpha-synuclein-enhanced green fluorescent protein fusion proteins form proteasome sensitive inclusions in primary neurons. Neuroscience. 2001;104:901-12 pubmed
Sharma N, Hewett J, Ozelius L, Ramesh V, McLean P, Breakefield X, et al. A close association of torsinA and alpha-synuclein in Lewy bodies: a fluorescence resonance energy transfer study. Am J Pathol. 2001;159:339-44 pubmed
Gomez Tortosa E, Newell K, Irizarry M, Sanders J, Hyman B. alpha-Synuclein immunoreactivity in dementia with Lewy bodies: morphological staging and comparison with ubiquitin immunostaining. Acta Neuropathol. 2000;99:352-7 pubmed
Serpell L, Berriman J, Jakes R, Goedert M, Crowther R. Fiber diffraction of synthetic alpha-synuclein filaments shows amyloid-like cross-beta conformation. Proc Natl Acad Sci U S A. 2000;97:4897-902 pubmed
Conway K, Harper J, Lansbury P. Accelerated in vitro fibril formation by a mutant alpha-synuclein linked to early-onset Parkinson disease. Nat Med. 1998;4:1318-20 pubmed
Davidson W, Jonas A, Clayton D, George J. Stabilization of alpha-synuclein secondary structure upon binding to synthetic membranes. J Biol Chem. 1998;273:9443-9 pubmed
Withers G, George J, Banker G, Clayton D. Delayed localization of synelfin (synuclein, NACP) to presynaptic terminals in cultured rat hippocampal neurons. Brain Res Dev Brain Res. 1997;99:87-94 pubmed
George J, Jin H, Woods W, Clayton D. Characterization of a novel protein regulated during the critical period for song learning in the zebra finch. Neuron. 1995;15:361-72 pubmed
product information
Internal ID :
8145
Name :
H3C
Depositor Name :
George, J.
Depositor Institution :
University of Illinois
Date Deposited :
2/14/12
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
synuclein, alpha & beta
Antigen Species :
Canary
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Avian,Human,Mouse,Rat
Species Tested (legacy) :
canary, zebra finch, human, mouse, rat
Initial Publication Pubmed ID :
7646890
Collections :
Human,Neurodevelopment
Search Keywords :
George, J., synuclein, alpha & beta, Canary, MIgG1, avian/human/rat/mouse, SNCA, synelfin, NACP, AB_2618046, monoclonal, Human/Neurodevelopment, Immunofluorescence/Western Blot/Immunoprecipitation/FFPE
Antigen Molecular Weight :
Predicted: 15 kDa; Apparent: 19 kDa
Gene :
SNCA
Alternate Gene Name(s) :
synelfin, NACP
Uniprot ID :
Q91448
Antibody Registry ID :
AB_2618046
Synonyms (Alternate Clone Names) :
H3
Immunogen :
C-terminal of Canary synuclein cojugated to KLH
Immunogen Sequence :
peptide (C-EMPPEEEYQDYEPEA)
Clonality :
Monoclonal
Epitope Mapped :
Yes
Epitope Location or Sequence :
C-terminal (C-EMPPEEEYQDYEPEA)
Epitope Map PubMed ID :
7646890
Recommended Applications :
FFPE,Immunofluorescence,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunofluorescence Pubmed IDs :
7646890 9088569 11438481 26177091 22647715 23624948 11440819 10787032 29249285
Western Blot Pubmed IDs :
7646890 9088569 11687285 11553616 12946570 23392688 22647715 11440819 9545270
Immunoprecipitation Pubmed IDs :
11687285
Pubmed IDs :
10781096 9809558
DSHB Growth Medium :
Iscove's
References (legacy) :
Neuron 15, 361-372.; Brain Res. Dev. Brain Res. 99, 87-94.; Mol. Brain Res. 95, 138-145.; J. Biol. Chem. 276, 41958-41962.; Neurosci. Lett. 349(2), 133-135.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US