p46 cell surface protein excluded from macropinocytic cup | Developmental Studies Hybridoma Bank H36 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

p46 cell surface protein excluded from macropinocytic cup

catalog :

H36

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

H36

reactivity :

Dictyostelium discoideum AX4

application :

western blot, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 9

Reference
Denoncourt A, Paquet V, Sedighi A, Charette S. Identification of Proteins Associated with Multilamellar Bodies Produced by Dictyostelium discoideum. PLoS ONE. 2016;11:e0158270 pubmed publisher
Paquet V, Charette S. Amoeba-resisting bacteria found in multilamellar bodies secreted by Dictyostelium discoideum: social amoebae can also package bacteria. FEMS Microbiol Ecol. 2016;92: pubmed publisher
Lima W, Vinet A, Pieters J, Cosson P. Role of PKD2 in rheotaxis in Dictyostelium. PLoS ONE. 2014;9:e88682 pubmed publisher
Vernay A, Cosson P. Immunofluorescence labeling of cell surface antigens in Dictyostelium. BMC Res Notes. 2013;6:317 pubmed publisher
Paquet V, Lessire R, Domergue F, Fouillen L, Filion G, Sedighi A, et al. Lipid composition of multilamellar bodies secreted by Dictyostelium discoideum reveals their amoebal origin. Eukaryot Cell. 2013;12:1326-34 pubmed publisher
Smith E, Lima W, Charette S, Cosson P. Effect of starvation on the endocytic pathway in Dictyostelium cells. Eukaryot Cell. 2010;9:387-92 pubmed publisher
Charette S, Cosson P. A LYST/beige homolog is involved in biogenesis of Dictyostelium secretory lysosomes. J Cell Sci. 2007;120:2338-43 pubmed
Charette S, Mercanti V, Letourneur F, Bennett N, Cosson P. A role for adaptor protein-3 complex in the organization of the endocytic pathway in Dictyostelium. Traffic. 2006;7:1528-38 pubmed
Mercanti V, Charette S, Bennett N, Ryckewaert J, Letourneur F, Cosson P. Selective membrane exclusion in phagocytic and macropinocytic cups. J Cell Sci. 2006;119:4079-87 pubmed

product information

Internal ID :

5778

Name :

H36

Depositor Name :

Cosson, P.

Depositor Institution :

Centre Medical Universitaire, Geneva

Date Deposited :

2/5/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

p46 cell surface protein excluded from macropinocytic cup

Antigen Species :

Dictyostelium

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Dictyostelium

Species Tested (legacy) :

Dictyostelium discoideum

Initial Publication Pubmed ID :

16968738

Collections :

Dictyostelium antigens

Search Keywords :

Cosson, P., p46 cell surface protein excluded from macropinocytic cup, Dictyostelium discoideum, MIgG1, Dictyostelium discoideum, cprG, CP7, AB_1553750, monoclonal, Dictyostelium antigens, Immunofluorescence/Western Blot/Immunoprecipitation/FACS

Antigen Molecular Weight :

Predicted: 47 kDa; Apparent: 46 kDa

Gene :

cprG

Alternate Gene Name(s) :

CP7

Uniprot ID :

Q94504

Antibody Registry ID :

AB_1553750

Immunogen :

Crude preparation of Dictyostelium membranes.

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

X63

Epitope Mapped :

Yes

Epitope Location or Sequence :

N-acetylglucosamine-1-phosphate

Epitope Map PubMed ID :

27340834

Recommended Applications :

FACS,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

16968738 17010123 17606989 23937900 23748431 20097741 26862140 27340834 24520414

Western Blot Pubmed IDs :

16968738 17010123 27340834

Immunoprecipitation Pubmed IDs :

16968738 27340834

FACS Pubmed IDs :

27340834

Additional Information :

H36 recognizes multiple proteins. The Cystein proteinase 7 (CP7) appears to be the main epitope of H36. H36 is also a marker of multilamellar bodies (MLB) where a different protein CDD (a protein containing a cytidine/deoxycytidylate deaminase (CDD) zinc-binding region) seems to be the target ( see [PMID: 27340834] for more details).

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Sci. 119, 4079-4087.

more info or order :

Developmental Studies Hybridoma Bank product webpage