product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Ganglioside GT1b
catalog :
GT1b-2b
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
GT1b-2b
reactivity :
mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry
citations: 8
Reference
Massaad C, Zhang G, Pillai L, Azhdarinia A, Liu W, Sheikh K. Fluorescently-tagged anti-ganglioside antibody selectively identifies peripheral nerve in living animals. Sci Rep. 2015;5:15766 pubmed publisher
Villanueva Cabello T, Mollicone R, Cruz Muñoz M, López Guerrero D, Martínez Duncker I. Activation of human naïve Th cells increases surface expression of GD3 and induces neoexpression of GD2 that colocalize with TCR clusters. Glycobiology. 2015;25:1454-64 pubmed publisher
Vajn K, Viljetić B, Degmecić I, Schnaar R, Heffer M. Differential distribution of major brain gangliosides in the adult mouse central nervous system. PLoS ONE. 2013;8:e75720 pubmed publisher
Viljetić B, Labak I, Majić S, Stambuk A, Heffer M. Distribution of mono-, di- and trisialo gangliosides in the brain of Actinopterygian fishes. Biochim Biophys Acta. 2012;1820:1437-43 pubmed publisher
Buchwald B, Zhang G, Vogt Eisele A, Zhang W, Ahangari R, Griffin J, et al. Anti-ganglioside antibodies alter presynaptic release and calcium influx. Neurobiol Dis. 2007;28:113-21 pubmed
Zhang G, Lopez P, Li C, Mehta N, Griffin J, Schnaar R, et al. Anti-ganglioside antibody-mediated neuronal cytotoxicity and its protection by intravenous immunoglobulin: implications for immune neuropathies. Brain. 2004;127:1085-100 pubmed
Gong Y, Tagawa Y, Lunn M, Laroy W, Heffer Lauc M, Li C, et al. Localization of major gangliosides in the PNS: implications for immune neuropathies. Brain. 2002;125:2491-506 pubmed
Schnaar R, Fromholt S, Gong Y, Vyas A, Laroy W, Wayman D, et al. Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides. Anal Biochem. 2002;302:276-84 pubmed
product information
Internal ID :
9409
Name :
GT1b-2b
Depositor Name :
Schnaar, R.L.
Depositor Institution :
Johns Hopkins School of Medicine
Date Deposited :
3/25/14
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
Ganglioside GT1b
Antigen Species :
Bovine
Host Species :
mouse
Isotype :
MIgG2b
Isotype for catalog (legacy) :
IgG2b
Positive Tested Species Reactivity :
Human,Mouse,Rat,Vertebrates
Species Tested (legacy) :
all vertebrates
Initial Publication Pubmed ID :
11878808
Depositor Notes (Special Instructions) :
GT1b-2b recognizes all vertebrate species. Paraformaldehyde fixation works well for immnostaining; avoid most detergents and organic solvents.
Collections :
Cell markers,Human,Neurodevelopment
Search Keywords :
Schnaar, R.L., Ganglioside GT1b, Bovine, MIgG2b, vertebrates/Human/Mouse/Rat, , , AB_2619575, monoclonal, Cell markers/Human/neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA
Antigen Molecular Weight :
2.15 kDa
Antibody Registry ID :
AB_2619575
Immunogen :
KLH-conjugated bovine brain GT1b
Clonality :
Monoclonal
Myeloma Strain :
P3x653
Epitope Mapped :
Yes
Epitope Location or Sequence :
GT1b
Epitope Map PubMed ID :
11878808
Recommended Applications :
ELISA,Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
11878808 24098718 22206893
Immunofluorescence Pubmed IDs :
12390975 24098718 17720506 26514366
Western Blot Pubmed IDs :
11878808 12390975
ELISA Pubmed IDs :
11878808
FACS Pubmed IDs :
26263924
Pubmed IDs :
14985267
Additional Information :
Gangliosides are glycosphingolipids with one or more sialic acids. Western blot references are for TLC immunooverlay.
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Neurochem. 75, 404-412.; Brain 125, 2491-2506.; Biochim. Biophys. Acta. 1686, 200-208.; J. Histochem. Cytochem. 55(8), 805-812.; PLoS ONE 8(9): e75720. doi:10.1371/journal.pone.0075720.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US